A protocol for the generation of patient-specific iPSC lines from peripheral blood mononuclear cells

The current procedure thoroughly explains how to reprogram induced pluripotent stem cells (iPSCs) from the patient’s peripheral blood mononuclear cells (PBMCs), a less invasive source; e.g., somatic cells. We describe how to isolate PBMCs and reprogram them into iPSCs by electroporation. Furthermore...

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Detalles Bibliográficos
Autores principales: Gao, Langping, Wang, Fangqin, Wang, Yan, Hu, Lidan, Mao, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287807/
https://www.ncbi.nlm.nih.gov/pubmed/35834385
http://dx.doi.org/10.1016/j.xpro.2022.101530
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author Gao, Langping
Wang, Fangqin
Wang, Yan
Hu, Lidan
Mao, Jianhua
author_facet Gao, Langping
Wang, Fangqin
Wang, Yan
Hu, Lidan
Mao, Jianhua
author_sort Gao, Langping
collection PubMed
description The current procedure thoroughly explains how to reprogram induced pluripotent stem cells (iPSCs) from the patient’s peripheral blood mononuclear cells (PBMCs), a less invasive source; e.g., somatic cells. We describe how to isolate PBMCs and reprogram them into iPSCs by electroporation. Furthermore, we provide an alternative approach to generating iPSC using Geltrex or Matrigel matrix to replace MEF-feeder. The challenge of this process is the relatively lower cell survival rates of PBMCs due to the damage of electroporation. For complete details on the use and execution of this protocol, please refer to Hu et al. (2021).
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spelling pubmed-92878072022-07-17 A protocol for the generation of patient-specific iPSC lines from peripheral blood mononuclear cells Gao, Langping Wang, Fangqin Wang, Yan Hu, Lidan Mao, Jianhua STAR Protoc Protocol The current procedure thoroughly explains how to reprogram induced pluripotent stem cells (iPSCs) from the patient’s peripheral blood mononuclear cells (PBMCs), a less invasive source; e.g., somatic cells. We describe how to isolate PBMCs and reprogram them into iPSCs by electroporation. Furthermore, we provide an alternative approach to generating iPSC using Geltrex or Matrigel matrix to replace MEF-feeder. The challenge of this process is the relatively lower cell survival rates of PBMCs due to the damage of electroporation. For complete details on the use and execution of this protocol, please refer to Hu et al. (2021). Elsevier 2022-07-13 /pmc/articles/PMC9287807/ /pubmed/35834385 http://dx.doi.org/10.1016/j.xpro.2022.101530 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Gao, Langping
Wang, Fangqin
Wang, Yan
Hu, Lidan
Mao, Jianhua
A protocol for the generation of patient-specific iPSC lines from peripheral blood mononuclear cells
title A protocol for the generation of patient-specific iPSC lines from peripheral blood mononuclear cells
title_full A protocol for the generation of patient-specific iPSC lines from peripheral blood mononuclear cells
title_fullStr A protocol for the generation of patient-specific iPSC lines from peripheral blood mononuclear cells
title_full_unstemmed A protocol for the generation of patient-specific iPSC lines from peripheral blood mononuclear cells
title_short A protocol for the generation of patient-specific iPSC lines from peripheral blood mononuclear cells
title_sort protocol for the generation of patient-specific ipsc lines from peripheral blood mononuclear cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287807/
https://www.ncbi.nlm.nih.gov/pubmed/35834385
http://dx.doi.org/10.1016/j.xpro.2022.101530
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