Isolation of mouse ovarian follicles for single-cell RNA-seq and in vitro culture

Here, we provide a protocol combing single-cell genomics and functional experiments to study stepwise folliculogenesis. This protocol isolates different developmental stages of mouse ovarian follicles to perform single-cell RNA-seq and in vitro culture, allowing to dissect and validate key molecular...

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Detalles Bibliográficos
Autores principales: Qian, Jingjing, Zhu, Ran, Yan, Rui, Long, Xin, Guo, Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287829/
https://www.ncbi.nlm.nih.gov/pubmed/35830306
http://dx.doi.org/10.1016/j.xpro.2022.101537

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