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PLA-lignin nanofibers as antioxidant biomaterials for cartilage regeneration and osteoarthritis treatment

BACKGROUND: Osteoarthritis (OA) is common musculoskeletal disorders associated with overgeneration of free radicals, and it causes joint pain, inflammation, and cartilage degradation. Lignin as a natural antioxidant biopolymer has shown its great potential for biomedical applications. In this work,...

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Autores principales: Liang, Ruiming, Yang, Xingchen, Yew, Pek Yin Michelle, Sugiarto, Sigit, Zhu, Qiang, Zhao, Jinmin, Loh, Xian Jun, Zheng, Li, Kai, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287996/
https://www.ncbi.nlm.nih.gov/pubmed/35842720
http://dx.doi.org/10.1186/s12951-022-01534-2
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author Liang, Ruiming
Yang, Xingchen
Yew, Pek Yin Michelle
Sugiarto, Sigit
Zhu, Qiang
Zhao, Jinmin
Loh, Xian Jun
Zheng, Li
Kai, Dan
author_facet Liang, Ruiming
Yang, Xingchen
Yew, Pek Yin Michelle
Sugiarto, Sigit
Zhu, Qiang
Zhao, Jinmin
Loh, Xian Jun
Zheng, Li
Kai, Dan
author_sort Liang, Ruiming
collection PubMed
description BACKGROUND: Osteoarthritis (OA) is common musculoskeletal disorders associated with overgeneration of free radicals, and it causes joint pain, inflammation, and cartilage degradation. Lignin as a natural antioxidant biopolymer has shown its great potential for biomedical applications. In this work, we developed a series of lignin-based nanofibers as antioxidative scaffolds for cartilage tissue engineering. RESULTS: The nanofibers were engineered by grafting poly(lactic acid) (PLA) into lignin via ring-opening polymerization and followed by electrospinning. Varying the lignin content in the system was able to adjust the physiochemical properties of the resulting nanofibers, including fiber diameters, mechanical and viscoelastic properties, and antioxidant activity. In vitro study demonstrated that the PLA-lignin nanofibers could protect bone marrow-derived mesenchymal stem/stromal cells (BMSCs) from oxidative stress and promote the chondrogenic differentiation. Moreover, the animal study showed that the lignin nanofibers could promote cartilage regeneration and repair cartilage defects within 6 weeks of implantation. CONCLUSION: Our study indicated that lignin-based nanofibers could serve as an antioxidant tissue engineering scaffold and facilitate the cartilage regrowth for OA treatment. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-022-01534-2.
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spelling pubmed-92879962022-07-17 PLA-lignin nanofibers as antioxidant biomaterials for cartilage regeneration and osteoarthritis treatment Liang, Ruiming Yang, Xingchen Yew, Pek Yin Michelle Sugiarto, Sigit Zhu, Qiang Zhao, Jinmin Loh, Xian Jun Zheng, Li Kai, Dan J Nanobiotechnology Research BACKGROUND: Osteoarthritis (OA) is common musculoskeletal disorders associated with overgeneration of free radicals, and it causes joint pain, inflammation, and cartilage degradation. Lignin as a natural antioxidant biopolymer has shown its great potential for biomedical applications. In this work, we developed a series of lignin-based nanofibers as antioxidative scaffolds for cartilage tissue engineering. RESULTS: The nanofibers were engineered by grafting poly(lactic acid) (PLA) into lignin via ring-opening polymerization and followed by electrospinning. Varying the lignin content in the system was able to adjust the physiochemical properties of the resulting nanofibers, including fiber diameters, mechanical and viscoelastic properties, and antioxidant activity. In vitro study demonstrated that the PLA-lignin nanofibers could protect bone marrow-derived mesenchymal stem/stromal cells (BMSCs) from oxidative stress and promote the chondrogenic differentiation. Moreover, the animal study showed that the lignin nanofibers could promote cartilage regeneration and repair cartilage defects within 6 weeks of implantation. CONCLUSION: Our study indicated that lignin-based nanofibers could serve as an antioxidant tissue engineering scaffold and facilitate the cartilage regrowth for OA treatment. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-022-01534-2. BioMed Central 2022-07-16 /pmc/articles/PMC9287996/ /pubmed/35842720 http://dx.doi.org/10.1186/s12951-022-01534-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Liang, Ruiming
Yang, Xingchen
Yew, Pek Yin Michelle
Sugiarto, Sigit
Zhu, Qiang
Zhao, Jinmin
Loh, Xian Jun
Zheng, Li
Kai, Dan
PLA-lignin nanofibers as antioxidant biomaterials for cartilage regeneration and osteoarthritis treatment
title PLA-lignin nanofibers as antioxidant biomaterials for cartilage regeneration and osteoarthritis treatment
title_full PLA-lignin nanofibers as antioxidant biomaterials for cartilage regeneration and osteoarthritis treatment
title_fullStr PLA-lignin nanofibers as antioxidant biomaterials for cartilage regeneration and osteoarthritis treatment
title_full_unstemmed PLA-lignin nanofibers as antioxidant biomaterials for cartilage regeneration and osteoarthritis treatment
title_short PLA-lignin nanofibers as antioxidant biomaterials for cartilage regeneration and osteoarthritis treatment
title_sort pla-lignin nanofibers as antioxidant biomaterials for cartilage regeneration and osteoarthritis treatment
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287996/
https://www.ncbi.nlm.nih.gov/pubmed/35842720
http://dx.doi.org/10.1186/s12951-022-01534-2
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