Cargando…

A New and Highly Sensitive Serum Mannoprotein Lateral Flow Assay for Point-of-Care Diagnosis of Invasive Aspergillosis (Tripathy Method)

Background and objectives The mannoprotein lateral flow assay (MP-LFA) or Aspergillus-specific lateral flow device (AspLFD) is a novel rapid test for point-of-care diagnosis (PoC) of invasive aspergillosis (IA), but its routine clinical application is hampered due to low sensitivity (S(n)) of the as...

Descripción completa

Detalles Bibliográficos
Autores principales: Chaturvedi, Chandra P, Hashim, Zia, Tripathy, Naresh K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cureus 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288649/
https://www.ncbi.nlm.nih.gov/pubmed/35859976
http://dx.doi.org/10.7759/cureus.26025
_version_ 1784748495815573504
author Chaturvedi, Chandra P
Hashim, Zia
Tripathy, Naresh K
author_facet Chaturvedi, Chandra P
Hashim, Zia
Tripathy, Naresh K
author_sort Chaturvedi, Chandra P
collection PubMed
description Background and objectives The mannoprotein lateral flow assay (MP-LFA) or Aspergillus-specific lateral flow device (AspLFD) is a novel rapid test for point-of-care diagnosis (PoC) of invasive aspergillosis (IA), but its routine clinical application is hampered due to low sensitivity (S(n)) of the assay in serum. Therefore, this study aimed to develop a new method to enhance the S(n )of the serum MP-LFA. Methodology In the new method (Tripathy method), we used direct heating of the serum without any dilution at 120(°)C for 15 minutes to purify the mannoprotein (MP) antigen of the Aspergillus. The MP-enriched serum supernatant obtained after centrifugation was loaded in an LFD cassette, and the results were read after 20 minutes using a digital cube reader. In parallel to our new method, AspLFD was performed according to the manufacturer’s instructions. The diagnostic performance of the two methods was evaluated using paired sera of true IA patients (IA, n=18) and healthy subjects (controls, n=20). The positivity of the two methods was also evaluated in the sera of leukemia patients with possible/probable IA (possible/probable IA; n=23). Results The Tripathy method had a significantly higher sensitivity (88.9% versus 55.5%; p<0.05) and diagnostic odds ratio (72.0 versus 23.7) than the standard AspLFD method. In receiver operating characteristic curve analysis for differentiation between IA patients and controls, although the Tripathy method (area under curve; AUC: 0.894, p<0.001) and AspLFD method (AUC: 0.753, p<0.001) were significantly associated with IA, the AUC of the Tripathy method was significantly higher than that of the AspLFD method (0.894 versus 0.753; p<0.05). In the sera of possible/probable IA, MP-LFA by the Tripathy method had a significantly higher rate of positivity than the AspLFD method (39.0% versus 21.7%; p<0.05). Conclusion Our data show that the Tripathy method is a highly sensitive method of MP-LFA for the PoC diagnosis of IA in clinical settings.
format Online
Article
Text
id pubmed-9288649
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Cureus
record_format MEDLINE/PubMed
spelling pubmed-92886492022-07-19 A New and Highly Sensitive Serum Mannoprotein Lateral Flow Assay for Point-of-Care Diagnosis of Invasive Aspergillosis (Tripathy Method) Chaturvedi, Chandra P Hashim, Zia Tripathy, Naresh K Cureus Emergency Medicine Background and objectives The mannoprotein lateral flow assay (MP-LFA) or Aspergillus-specific lateral flow device (AspLFD) is a novel rapid test for point-of-care diagnosis (PoC) of invasive aspergillosis (IA), but its routine clinical application is hampered due to low sensitivity (S(n)) of the assay in serum. Therefore, this study aimed to develop a new method to enhance the S(n )of the serum MP-LFA. Methodology In the new method (Tripathy method), we used direct heating of the serum without any dilution at 120(°)C for 15 minutes to purify the mannoprotein (MP) antigen of the Aspergillus. The MP-enriched serum supernatant obtained after centrifugation was loaded in an LFD cassette, and the results were read after 20 minutes using a digital cube reader. In parallel to our new method, AspLFD was performed according to the manufacturer’s instructions. The diagnostic performance of the two methods was evaluated using paired sera of true IA patients (IA, n=18) and healthy subjects (controls, n=20). The positivity of the two methods was also evaluated in the sera of leukemia patients with possible/probable IA (possible/probable IA; n=23). Results The Tripathy method had a significantly higher sensitivity (88.9% versus 55.5%; p<0.05) and diagnostic odds ratio (72.0 versus 23.7) than the standard AspLFD method. In receiver operating characteristic curve analysis for differentiation between IA patients and controls, although the Tripathy method (area under curve; AUC: 0.894, p<0.001) and AspLFD method (AUC: 0.753, p<0.001) were significantly associated with IA, the AUC of the Tripathy method was significantly higher than that of the AspLFD method (0.894 versus 0.753; p<0.05). In the sera of possible/probable IA, MP-LFA by the Tripathy method had a significantly higher rate of positivity than the AspLFD method (39.0% versus 21.7%; p<0.05). Conclusion Our data show that the Tripathy method is a highly sensitive method of MP-LFA for the PoC diagnosis of IA in clinical settings. Cureus 2022-06-17 /pmc/articles/PMC9288649/ /pubmed/35859976 http://dx.doi.org/10.7759/cureus.26025 Text en Copyright © 2022, Chaturvedi et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Emergency Medicine
Chaturvedi, Chandra P
Hashim, Zia
Tripathy, Naresh K
A New and Highly Sensitive Serum Mannoprotein Lateral Flow Assay for Point-of-Care Diagnosis of Invasive Aspergillosis (Tripathy Method)
title A New and Highly Sensitive Serum Mannoprotein Lateral Flow Assay for Point-of-Care Diagnosis of Invasive Aspergillosis (Tripathy Method)
title_full A New and Highly Sensitive Serum Mannoprotein Lateral Flow Assay for Point-of-Care Diagnosis of Invasive Aspergillosis (Tripathy Method)
title_fullStr A New and Highly Sensitive Serum Mannoprotein Lateral Flow Assay for Point-of-Care Diagnosis of Invasive Aspergillosis (Tripathy Method)
title_full_unstemmed A New and Highly Sensitive Serum Mannoprotein Lateral Flow Assay for Point-of-Care Diagnosis of Invasive Aspergillosis (Tripathy Method)
title_short A New and Highly Sensitive Serum Mannoprotein Lateral Flow Assay for Point-of-Care Diagnosis of Invasive Aspergillosis (Tripathy Method)
title_sort new and highly sensitive serum mannoprotein lateral flow assay for point-of-care diagnosis of invasive aspergillosis (tripathy method)
topic Emergency Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288649/
https://www.ncbi.nlm.nih.gov/pubmed/35859976
http://dx.doi.org/10.7759/cureus.26025
work_keys_str_mv AT chaturvedichandrap anewandhighlysensitiveserummannoproteinlateralflowassayforpointofcarediagnosisofinvasiveaspergillosistripathymethod
AT hashimzia anewandhighlysensitiveserummannoproteinlateralflowassayforpointofcarediagnosisofinvasiveaspergillosistripathymethod
AT tripathynareshk anewandhighlysensitiveserummannoproteinlateralflowassayforpointofcarediagnosisofinvasiveaspergillosistripathymethod
AT chaturvedichandrap newandhighlysensitiveserummannoproteinlateralflowassayforpointofcarediagnosisofinvasiveaspergillosistripathymethod
AT hashimzia newandhighlysensitiveserummannoproteinlateralflowassayforpointofcarediagnosisofinvasiveaspergillosistripathymethod
AT tripathynareshk newandhighlysensitiveserummannoproteinlateralflowassayforpointofcarediagnosisofinvasiveaspergillosistripathymethod