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Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers ( Solanum tuberosum )

MicroRNAs (miRNAs) are types of endogenous non‐coding small RNAs found in eukaryotes that are 18–25 nucleotides long. miRNAs are considered to be key regulatory factors of the expression of target mRNA. The roles of miRNAs involved in the regulation of anthocyanin accumulation in pigmented potatoes...

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Autores principales: Wu, Xiaojuan, Ma, Yanhong, Wu, Juan, Wang, Peijie, Zhang, Zhicheng, Xie, Rui, Liu, Jie, Fan, Bobo, Wei, Wei, Nie, Li Zhen, Liu, Xuting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289217/
https://www.ncbi.nlm.nih.gov/pubmed/35865074
http://dx.doi.org/10.1002/pld3.418
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author Wu, Xiaojuan
Ma, Yanhong
Wu, Juan
Wang, Peijie
Zhang, Zhicheng
Xie, Rui
Liu, Jie
Fan, Bobo
Wei, Wei
Nie, Li Zhen
Liu, Xuting
author_facet Wu, Xiaojuan
Ma, Yanhong
Wu, Juan
Wang, Peijie
Zhang, Zhicheng
Xie, Rui
Liu, Jie
Fan, Bobo
Wei, Wei
Nie, Li Zhen
Liu, Xuting
author_sort Wu, Xiaojuan
collection PubMed
description MicroRNAs (miRNAs) are types of endogenous non‐coding small RNAs found in eukaryotes that are 18–25 nucleotides long. miRNAs are considered to be key regulatory factors of the expression of target mRNA. The roles of miRNAs involved in the regulation of anthocyanin accumulation in pigmented potatoes have not been systematically reported. In this study, the differentially expressed miRNAs and their target genes involved in the accumulation of anthocyanin during different developmental stages in purple potato ( Solanum tuberosum L.) were identified using small RNA (sRNA) and degradome sequencing. A total of 275 differentially expressed miRNAs were identified in the sRNA libraries. A total of 69,387,200 raw reads were obtained from three degradome libraries. The anthocyanin responsive miRNA–mRNA modules were analyzed, and 37 miRNAs and 23 target genes were obtained. Different miRNAs regulate the key enzymes of anthocyanin synthesis in purple potato. The structural genes included phenylalanine ammonia lyase, chalcone isomerase, flavanone 3‐hydroxylase, and anthocyanidin 3‐O‐glucosyltransferase. The regulatory genes included WD40, MYB, and SPL9. stu‐miR172e‐5p_L‐1R‐1, stu‐miR828a, stu‐miR29b‐4‐5p, stu‐miR8019‐5p_L‐4R‐3, stu‐miR396b‐5p, stu‐miR5303f_L‐7R + 2, stu‐miR7997a_L‐3, stu‐miR7997b_L‐3, stu‐miR7997c_L + 3R‐5_2ss2TA3AG, stu‐miR156f‐5p_L + 1, stu‐miR156a, stu‐miR156a_R‐1, stu‐miR156e, stu‐miR858, stu‐miR5021, stu‐miR828 and their target genes were validated by qRT‐PCR. They play important roles in the coloration and accumulation of purple potatoes. These results provide new insights into the biosynthesis of anthocyanins in pigmented potatoes.
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spelling pubmed-92892172022-07-20 Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers ( Solanum tuberosum ) Wu, Xiaojuan Ma, Yanhong Wu, Juan Wang, Peijie Zhang, Zhicheng Xie, Rui Liu, Jie Fan, Bobo Wei, Wei Nie, Li Zhen Liu, Xuting Plant Direct Original Research MicroRNAs (miRNAs) are types of endogenous non‐coding small RNAs found in eukaryotes that are 18–25 nucleotides long. miRNAs are considered to be key regulatory factors of the expression of target mRNA. The roles of miRNAs involved in the regulation of anthocyanin accumulation in pigmented potatoes have not been systematically reported. In this study, the differentially expressed miRNAs and their target genes involved in the accumulation of anthocyanin during different developmental stages in purple potato ( Solanum tuberosum L.) were identified using small RNA (sRNA) and degradome sequencing. A total of 275 differentially expressed miRNAs were identified in the sRNA libraries. A total of 69,387,200 raw reads were obtained from three degradome libraries. The anthocyanin responsive miRNA–mRNA modules were analyzed, and 37 miRNAs and 23 target genes were obtained. Different miRNAs regulate the key enzymes of anthocyanin synthesis in purple potato. The structural genes included phenylalanine ammonia lyase, chalcone isomerase, flavanone 3‐hydroxylase, and anthocyanidin 3‐O‐glucosyltransferase. The regulatory genes included WD40, MYB, and SPL9. stu‐miR172e‐5p_L‐1R‐1, stu‐miR828a, stu‐miR29b‐4‐5p, stu‐miR8019‐5p_L‐4R‐3, stu‐miR396b‐5p, stu‐miR5303f_L‐7R + 2, stu‐miR7997a_L‐3, stu‐miR7997b_L‐3, stu‐miR7997c_L + 3R‐5_2ss2TA3AG, stu‐miR156f‐5p_L + 1, stu‐miR156a, stu‐miR156a_R‐1, stu‐miR156e, stu‐miR858, stu‐miR5021, stu‐miR828 and their target genes were validated by qRT‐PCR. They play important roles in the coloration and accumulation of purple potatoes. These results provide new insights into the biosynthesis of anthocyanins in pigmented potatoes. John Wiley and Sons Inc. 2022-07-17 /pmc/articles/PMC9289217/ /pubmed/35865074 http://dx.doi.org/10.1002/pld3.418 Text en © 2022 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Research
Wu, Xiaojuan
Ma, Yanhong
Wu, Juan
Wang, Peijie
Zhang, Zhicheng
Xie, Rui
Liu, Jie
Fan, Bobo
Wei, Wei
Nie, Li Zhen
Liu, Xuting
Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers ( Solanum tuberosum )
title Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers ( Solanum tuberosum )
title_full Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers ( Solanum tuberosum )
title_fullStr Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers ( Solanum tuberosum )
title_full_unstemmed Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers ( Solanum tuberosum )
title_short Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers ( Solanum tuberosum )
title_sort identification of micrornas and their target genes related to the accumulation of anthocyanin in purple potato tubers ( solanum tuberosum )
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289217/
https://www.ncbi.nlm.nih.gov/pubmed/35865074
http://dx.doi.org/10.1002/pld3.418
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