Cargando…

A Novel Technique Identifies Valve-Like Pathways Entering and Exiting Schlemm’s Canal in Macaca nemestrina Primates With Similarities to Human Pathways

Purpose: The aim of the study was 1) to describe a novel combination of techniques that permit immunohistochemistry imaging of Schlemm’s canal inlet (SIV) and outlet (SOV) valve-like structures, 2) to identify tissue-level SIV adhesive relationships linking the trabecular meshwork (TM) to hinged col...

Descripción completa

Detalles Bibliográficos
Autores principales: Martin, Elizabeth A., Johnstone, Murray A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289287/
https://www.ncbi.nlm.nih.gov/pubmed/35859902
http://dx.doi.org/10.3389/fcell.2022.868029
_version_ 1784748632098996224
author Martin, Elizabeth A.
Johnstone, Murray A.
author_facet Martin, Elizabeth A.
Johnstone, Murray A.
author_sort Martin, Elizabeth A.
collection PubMed
description Purpose: The aim of the study was 1) to describe a novel combination of techniques that permit immunohistochemistry imaging of Schlemm’s canal inlet (SIV) and outlet (SOV) valve-like structures, 2) to identify tissue-level SIV adhesive relationships linking the trabecular meshwork (TM) to hinged collagen leaflets at the Schlemm’s canal (SC) external wall, and 3) to determine whether the SIV lumen wall’s adhesive vascular markers are similar to those of the SC inner wall endothelium. Materials and Methods: Anterior segments of 16 M. nemestrina primates underwent immunohistochemistry (IHC) labeling. We perfused fluorescent microspheres into 12 of the eyes. Limbal tissues were divided into quadrants, viscoelastic introduced into SC, tissues fixed, immunohistochemistry performed, radial segments cut, tissues clarified, and confocal microscopy performed. Finally, we generated ImageJ 3D projections encompassing the TM, SC, and distal pathways. Results: IHC imaging identified 3D relationships between SIV, collector channel ostia, collector channels (CC), SOV, and intrascleral channels. Imaging depth increased 176.9%, following clarification (p < 0.0001). Imaging demonstrated CD31, collagen type 1 and 4 in the walls of the SIV lumen and more distal pathways. In eight eyes, 384 segments were examined, 447 SIV identified, and 15.4% contained microspheres. Conclusion: Our technique’s imaging depth permitted the identification of SIV linkage between the TM and SOV. We found comparable cell–cell adhesion molecules (CD31) and basement membrane components in the SC inner wall and SIV lumen walls. Recent OCT studies have suggested that SIV tensional relationships may control CC entrance dimensions that regulate distal resistance. Cellular adhesive properties sustain SIV tensional relationships. These SIV cell–cell and cell-basement membrane properties warrant further study because abnormalities could be a factor in the IOP elevation of glaucoma.
format Online
Article
Text
id pubmed-9289287
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92892872022-07-19 A Novel Technique Identifies Valve-Like Pathways Entering and Exiting Schlemm’s Canal in Macaca nemestrina Primates With Similarities to Human Pathways Martin, Elizabeth A. Johnstone, Murray A. Front Cell Dev Biol Cell and Developmental Biology Purpose: The aim of the study was 1) to describe a novel combination of techniques that permit immunohistochemistry imaging of Schlemm’s canal inlet (SIV) and outlet (SOV) valve-like structures, 2) to identify tissue-level SIV adhesive relationships linking the trabecular meshwork (TM) to hinged collagen leaflets at the Schlemm’s canal (SC) external wall, and 3) to determine whether the SIV lumen wall’s adhesive vascular markers are similar to those of the SC inner wall endothelium. Materials and Methods: Anterior segments of 16 M. nemestrina primates underwent immunohistochemistry (IHC) labeling. We perfused fluorescent microspheres into 12 of the eyes. Limbal tissues were divided into quadrants, viscoelastic introduced into SC, tissues fixed, immunohistochemistry performed, radial segments cut, tissues clarified, and confocal microscopy performed. Finally, we generated ImageJ 3D projections encompassing the TM, SC, and distal pathways. Results: IHC imaging identified 3D relationships between SIV, collector channel ostia, collector channels (CC), SOV, and intrascleral channels. Imaging depth increased 176.9%, following clarification (p < 0.0001). Imaging demonstrated CD31, collagen type 1 and 4 in the walls of the SIV lumen and more distal pathways. In eight eyes, 384 segments were examined, 447 SIV identified, and 15.4% contained microspheres. Conclusion: Our technique’s imaging depth permitted the identification of SIV linkage between the TM and SOV. We found comparable cell–cell adhesion molecules (CD31) and basement membrane components in the SC inner wall and SIV lumen walls. Recent OCT studies have suggested that SIV tensional relationships may control CC entrance dimensions that regulate distal resistance. Cellular adhesive properties sustain SIV tensional relationships. These SIV cell–cell and cell-basement membrane properties warrant further study because abnormalities could be a factor in the IOP elevation of glaucoma. Frontiers Media S.A. 2022-07-04 /pmc/articles/PMC9289287/ /pubmed/35859902 http://dx.doi.org/10.3389/fcell.2022.868029 Text en Copyright © 2022 Martin and Johnstone. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Martin, Elizabeth A.
Johnstone, Murray A.
A Novel Technique Identifies Valve-Like Pathways Entering and Exiting Schlemm’s Canal in Macaca nemestrina Primates With Similarities to Human Pathways
title A Novel Technique Identifies Valve-Like Pathways Entering and Exiting Schlemm’s Canal in Macaca nemestrina Primates With Similarities to Human Pathways
title_full A Novel Technique Identifies Valve-Like Pathways Entering and Exiting Schlemm’s Canal in Macaca nemestrina Primates With Similarities to Human Pathways
title_fullStr A Novel Technique Identifies Valve-Like Pathways Entering and Exiting Schlemm’s Canal in Macaca nemestrina Primates With Similarities to Human Pathways
title_full_unstemmed A Novel Technique Identifies Valve-Like Pathways Entering and Exiting Schlemm’s Canal in Macaca nemestrina Primates With Similarities to Human Pathways
title_short A Novel Technique Identifies Valve-Like Pathways Entering and Exiting Schlemm’s Canal in Macaca nemestrina Primates With Similarities to Human Pathways
title_sort novel technique identifies valve-like pathways entering and exiting schlemm’s canal in macaca nemestrina primates with similarities to human pathways
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289287/
https://www.ncbi.nlm.nih.gov/pubmed/35859902
http://dx.doi.org/10.3389/fcell.2022.868029
work_keys_str_mv AT martinelizabetha anoveltechniqueidentifiesvalvelikepathwaysenteringandexitingschlemmscanalinmacacanemestrinaprimateswithsimilaritiestohumanpathways
AT johnstonemurraya anoveltechniqueidentifiesvalvelikepathwaysenteringandexitingschlemmscanalinmacacanemestrinaprimateswithsimilaritiestohumanpathways
AT martinelizabetha noveltechniqueidentifiesvalvelikepathwaysenteringandexitingschlemmscanalinmacacanemestrinaprimateswithsimilaritiestohumanpathways
AT johnstonemurraya noveltechniqueidentifiesvalvelikepathwaysenteringandexitingschlemmscanalinmacacanemestrinaprimateswithsimilaritiestohumanpathways