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Early Transcriptional Response to Monensin in Sensitive and Resistant Strains of Eimeria tenella

Eimeria parasites are the causative agents of coccidiosis, a common parasitic disease in poultry and livestock that causes significant economic losses to the animal husbandry industry. Ionophore coccidiostats, such as monensin and salinomycin, are widely used for prophylaxis of coccidiosis in poultr...

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Autores principales: Zhang, Hongtao, Zhang, Lei, Si, Hongbin, Liu, Xianyong, Suo, Xun, Hu, Dandan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289555/
https://www.ncbi.nlm.nih.gov/pubmed/35859739
http://dx.doi.org/10.3389/fmicb.2022.934153
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author Zhang, Hongtao
Zhang, Lei
Si, Hongbin
Liu, Xianyong
Suo, Xun
Hu, Dandan
author_facet Zhang, Hongtao
Zhang, Lei
Si, Hongbin
Liu, Xianyong
Suo, Xun
Hu, Dandan
author_sort Zhang, Hongtao
collection PubMed
description Eimeria parasites are the causative agents of coccidiosis, a common parasitic disease in poultry and livestock that causes significant economic losses to the animal husbandry industry. Ionophore coccidiostats, such as monensin and salinomycin, are widely used for prophylaxis of coccidiosis in poultry. Unfortunately, widespread drug resistance has compromised their efficacy. As a result, there is an increasing need to understand the targets and resistance mechanisms to anticoccidials. However, how Eimeria parasite genes respond to ionophores remains unclear. In this study, resistance to monensin was induced in E. tenella through serial generations of selection. Both sensitive and resistant E. tenella sporozoites were treated with 5 μg/ml monensin for 0, 2, and 4 h, respectively. Gene transcription profiles were then compared by high-throughput sequencing. The results showed that protein translation-related genes were significantly downregulated after drug induction. A total of 1,848 DEGs were detected in the sensitive strain after 2 h of exposure, whereas only 31 were detected in the resistant strain. Among these DEGs in the sensitive strain, genes associated with protein degradation were significantly upregulated, supporting the autophagy-like parasite killing theory. Then, 4 h of exposure resulted in additional 626 and 621 DEGs for sensitive and resistant strains, respectively. This result implies that the gene transcription in sensitive strain is more susceptible to monensin treatment. Our results provide gene expression landscapes of E. tenella following monensin treatment. These data will contribute to a better understanding of the mechanism of drug resistance to polyether ionophores in coccidia.
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spelling pubmed-92895552022-07-19 Early Transcriptional Response to Monensin in Sensitive and Resistant Strains of Eimeria tenella Zhang, Hongtao Zhang, Lei Si, Hongbin Liu, Xianyong Suo, Xun Hu, Dandan Front Microbiol Microbiology Eimeria parasites are the causative agents of coccidiosis, a common parasitic disease in poultry and livestock that causes significant economic losses to the animal husbandry industry. Ionophore coccidiostats, such as monensin and salinomycin, are widely used for prophylaxis of coccidiosis in poultry. Unfortunately, widespread drug resistance has compromised their efficacy. As a result, there is an increasing need to understand the targets and resistance mechanisms to anticoccidials. However, how Eimeria parasite genes respond to ionophores remains unclear. In this study, resistance to monensin was induced in E. tenella through serial generations of selection. Both sensitive and resistant E. tenella sporozoites were treated with 5 μg/ml monensin for 0, 2, and 4 h, respectively. Gene transcription profiles were then compared by high-throughput sequencing. The results showed that protein translation-related genes were significantly downregulated after drug induction. A total of 1,848 DEGs were detected in the sensitive strain after 2 h of exposure, whereas only 31 were detected in the resistant strain. Among these DEGs in the sensitive strain, genes associated with protein degradation were significantly upregulated, supporting the autophagy-like parasite killing theory. Then, 4 h of exposure resulted in additional 626 and 621 DEGs for sensitive and resistant strains, respectively. This result implies that the gene transcription in sensitive strain is more susceptible to monensin treatment. Our results provide gene expression landscapes of E. tenella following monensin treatment. These data will contribute to a better understanding of the mechanism of drug resistance to polyether ionophores in coccidia. Frontiers Media S.A. 2022-07-04 /pmc/articles/PMC9289555/ /pubmed/35859739 http://dx.doi.org/10.3389/fmicb.2022.934153 Text en Copyright © 2022 Zhang, Zhang, Si, Liu, Suo and Hu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Hongtao
Zhang, Lei
Si, Hongbin
Liu, Xianyong
Suo, Xun
Hu, Dandan
Early Transcriptional Response to Monensin in Sensitive and Resistant Strains of Eimeria tenella
title Early Transcriptional Response to Monensin in Sensitive and Resistant Strains of Eimeria tenella
title_full Early Transcriptional Response to Monensin in Sensitive and Resistant Strains of Eimeria tenella
title_fullStr Early Transcriptional Response to Monensin in Sensitive and Resistant Strains of Eimeria tenella
title_full_unstemmed Early Transcriptional Response to Monensin in Sensitive and Resistant Strains of Eimeria tenella
title_short Early Transcriptional Response to Monensin in Sensitive and Resistant Strains of Eimeria tenella
title_sort early transcriptional response to monensin in sensitive and resistant strains of eimeria tenella
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289555/
https://www.ncbi.nlm.nih.gov/pubmed/35859739
http://dx.doi.org/10.3389/fmicb.2022.934153
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