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Dataset of 16S rRNA and alkB genes in hydrocarbon polluted soils of Kuwait as revealed by Pyrosequencing

The data in this article was generated by high throughput sequencing of moderately hydrocarbon polluted sites (S1 and S2) and a heavily polluted site (S3) in Kuwait. Deoxyribonucleic acid (DNA) extracted from each site was subjected to polymerase chain reaction (PCR) amplification employing conserve...

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Autores principales: Al-Momin, Sabah, Habibi, Nazima, Rahmeh, Rita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289844/
https://www.ncbi.nlm.nih.gov/pubmed/35859785
http://dx.doi.org/10.1016/j.dib.2022.108434
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author Al-Momin, Sabah
Habibi, Nazima
Rahmeh, Rita
author_facet Al-Momin, Sabah
Habibi, Nazima
Rahmeh, Rita
author_sort Al-Momin, Sabah
collection PubMed
description The data in this article was generated by high throughput sequencing of moderately hydrocarbon polluted sites (S1 and S2) and a heavily polluted site (S3) in Kuwait. Deoxyribonucleic acid (DNA) extracted from each site was subjected to polymerase chain reaction (PCR) amplification employing conserved primers of 16S rRNA and alkB genes. Unique Molecular Identifiers (MID) tags were added to individual samples prior to pooling and sequencing on a Roche GS FLX platform using Pyrosequencing Titanium Chemistry. Raw sff files were deposited to the public repository of National Centre for Biotechnology Information (NCBI) under accession no PRJNA816075. The sff files were clipped according to the MID tags and converted to fasta format. 16S rRNA gene sequences were aligned against the SILVA database. The predominant genera at S1 and S2 was Alkanindiges whereas Alcanivorax, was highly abundant at S3. Alkanindiges have been found to play a key role in hydrocarbon degradation and Alcanivorax genus is known for its hydrocarbon degrading capability. The alk B gene sequences were subjected to blastx. The diversity of alkB gene was higher in S3 as compared to S1 and S2. These findings may open the way to the use of the genera Alkanindiges and Alcanivorax in the rehabilitation of hydrocarbon-contaminated sites in hot, arid climates. The isolation of these microorganisms and the design of bioaugmentation procedures specific to the dry climate could be a key step towards the restoration of hydrocarbon contaminated soils.
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spelling pubmed-92898442022-07-19 Dataset of 16S rRNA and alkB genes in hydrocarbon polluted soils of Kuwait as revealed by Pyrosequencing Al-Momin, Sabah Habibi, Nazima Rahmeh, Rita Data Brief Data Article The data in this article was generated by high throughput sequencing of moderately hydrocarbon polluted sites (S1 and S2) and a heavily polluted site (S3) in Kuwait. Deoxyribonucleic acid (DNA) extracted from each site was subjected to polymerase chain reaction (PCR) amplification employing conserved primers of 16S rRNA and alkB genes. Unique Molecular Identifiers (MID) tags were added to individual samples prior to pooling and sequencing on a Roche GS FLX platform using Pyrosequencing Titanium Chemistry. Raw sff files were deposited to the public repository of National Centre for Biotechnology Information (NCBI) under accession no PRJNA816075. The sff files were clipped according to the MID tags and converted to fasta format. 16S rRNA gene sequences were aligned against the SILVA database. The predominant genera at S1 and S2 was Alkanindiges whereas Alcanivorax, was highly abundant at S3. Alkanindiges have been found to play a key role in hydrocarbon degradation and Alcanivorax genus is known for its hydrocarbon degrading capability. The alk B gene sequences were subjected to blastx. The diversity of alkB gene was higher in S3 as compared to S1 and S2. These findings may open the way to the use of the genera Alkanindiges and Alcanivorax in the rehabilitation of hydrocarbon-contaminated sites in hot, arid climates. The isolation of these microorganisms and the design of bioaugmentation procedures specific to the dry climate could be a key step towards the restoration of hydrocarbon contaminated soils. Elsevier 2022-07-03 /pmc/articles/PMC9289844/ /pubmed/35859785 http://dx.doi.org/10.1016/j.dib.2022.108434 Text en © 2022 The Author(s). Published by Elsevier Inc. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Al-Momin, Sabah
Habibi, Nazima
Rahmeh, Rita
Dataset of 16S rRNA and alkB genes in hydrocarbon polluted soils of Kuwait as revealed by Pyrosequencing
title Dataset of 16S rRNA and alkB genes in hydrocarbon polluted soils of Kuwait as revealed by Pyrosequencing
title_full Dataset of 16S rRNA and alkB genes in hydrocarbon polluted soils of Kuwait as revealed by Pyrosequencing
title_fullStr Dataset of 16S rRNA and alkB genes in hydrocarbon polluted soils of Kuwait as revealed by Pyrosequencing
title_full_unstemmed Dataset of 16S rRNA and alkB genes in hydrocarbon polluted soils of Kuwait as revealed by Pyrosequencing
title_short Dataset of 16S rRNA and alkB genes in hydrocarbon polluted soils of Kuwait as revealed by Pyrosequencing
title_sort dataset of 16s rrna and alkb genes in hydrocarbon polluted soils of kuwait as revealed by pyrosequencing
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289844/
https://www.ncbi.nlm.nih.gov/pubmed/35859785
http://dx.doi.org/10.1016/j.dib.2022.108434
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