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The effects of valproic acid on skin healing: experimental study in rats
PURPOSE: To recognize the effects of valproic acid (VPA), an epigenetic drug, on the skin healing process. METHODS: Sixty male Wistar rats were divided into two groups: the experiment treated with VPA (100 mg/kg/day); and the control, with 0.9% sodium chloride by gavage. Skin healing was studied in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9290763/ https://www.ncbi.nlm.nih.gov/pubmed/35857935 http://dx.doi.org/10.1590/acb370403 |
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author | Biondo-Simões, Rachel Biondo-Simões, Maria de Lourdes Pessole Ioshii, Sérgio Ossamu Robes, Rogério Ribeiro Dall’Antonia, Moacir de Oliveira |
author_facet | Biondo-Simões, Rachel Biondo-Simões, Maria de Lourdes Pessole Ioshii, Sérgio Ossamu Robes, Rogério Ribeiro Dall’Antonia, Moacir de Oliveira |
author_sort | Biondo-Simões, Rachel |
collection | PubMed |
description | PURPOSE: To recognize the effects of valproic acid (VPA), an epigenetic drug, on the skin healing process. METHODS: Sixty male Wistar rats were divided into two groups: the experiment treated with VPA (100 mg/kg/day); and the control, with 0.9% sodium chloride by gavage. Skin healing was studied in three moments (the third, the seventh, and the 14th day), evaluating the parameters: inflammatory reaction and its intensity (anti-LCA), angiogenesis (anti-CD34), collagen I and III (anti-collagen I, anti-collagen III and Picrosirius-red F3BA) and myofibroblasts (anti-alpha-AMS). RESULTS: The inflammatory reaction was acute or sub-acute in both groups on the third day. On the seventh and the 14th day, chronic predominated in the control (p=0.006), and sub-acute in the experiment (p=0.020). There was a greater number of leukocytes in the group treated only on the third day (p=0.036). The number of vessels was lower in the treated group at the three times (p3=0.002, p7<0.001, and p14=0.027). Myofibroblasts were rare in the third day and moderate quantity in the remaining periods. Collagen I density was higher in the control at the three times (p<0.001) and collagen III in the treated group (p<0.001). CONCLUSIONS: VPA led to a more intense inflammatory reaction, decreased angiogenesis and collagen deposition, especially type I collagen. |
format | Online Article Text |
id | pubmed-9290763 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
record_format | MEDLINE/PubMed |
spelling | pubmed-92907632022-07-29 The effects of valproic acid on skin healing: experimental study in rats Biondo-Simões, Rachel Biondo-Simões, Maria de Lourdes Pessole Ioshii, Sérgio Ossamu Robes, Rogério Ribeiro Dall’Antonia, Moacir de Oliveira Acta Cir Bras Original Article PURPOSE: To recognize the effects of valproic acid (VPA), an epigenetic drug, on the skin healing process. METHODS: Sixty male Wistar rats were divided into two groups: the experiment treated with VPA (100 mg/kg/day); and the control, with 0.9% sodium chloride by gavage. Skin healing was studied in three moments (the third, the seventh, and the 14th day), evaluating the parameters: inflammatory reaction and its intensity (anti-LCA), angiogenesis (anti-CD34), collagen I and III (anti-collagen I, anti-collagen III and Picrosirius-red F3BA) and myofibroblasts (anti-alpha-AMS). RESULTS: The inflammatory reaction was acute or sub-acute in both groups on the third day. On the seventh and the 14th day, chronic predominated in the control (p=0.006), and sub-acute in the experiment (p=0.020). There was a greater number of leukocytes in the group treated only on the third day (p=0.036). The number of vessels was lower in the treated group at the three times (p3=0.002, p7<0.001, and p14=0.027). Myofibroblasts were rare in the third day and moderate quantity in the remaining periods. Collagen I density was higher in the control at the three times (p<0.001) and collagen III in the treated group (p<0.001). CONCLUSIONS: VPA led to a more intense inflammatory reaction, decreased angiogenesis and collagen deposition, especially type I collagen. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2022-07-15 /pmc/articles/PMC9290763/ /pubmed/35857935 http://dx.doi.org/10.1590/acb370403 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Biondo-Simões, Rachel Biondo-Simões, Maria de Lourdes Pessole Ioshii, Sérgio Ossamu Robes, Rogério Ribeiro Dall’Antonia, Moacir de Oliveira The effects of valproic acid on skin healing: experimental study in rats |
title | The effects of valproic acid on skin healing: experimental study in rats |
title_full | The effects of valproic acid on skin healing: experimental study in rats |
title_fullStr | The effects of valproic acid on skin healing: experimental study in rats |
title_full_unstemmed | The effects of valproic acid on skin healing: experimental study in rats |
title_short | The effects of valproic acid on skin healing: experimental study in rats |
title_sort | effects of valproic acid on skin healing: experimental study in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9290763/ https://www.ncbi.nlm.nih.gov/pubmed/35857935 http://dx.doi.org/10.1590/acb370403 |
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