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Establishment of cultured primary acral melanoma cells and animal models for Chinese patients
Acral melanoma is the major subtype of melanoma in Chinese patients. However, a majority of current studies focused on non‐acral melanoma. Most immortalized melanoma cell lines and primary cells were not from acral melanoma. Besides, there are rarely reports about methods for establishing primary ac...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291188/ https://www.ncbi.nlm.nih.gov/pubmed/34097822 http://dx.doi.org/10.1111/pcmr.12996 |
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author | Hu, Rui Zhao, Shuang Su, Juan Chen, Xiang Yin, Mingzhu |
author_facet | Hu, Rui Zhao, Shuang Su, Juan Chen, Xiang Yin, Mingzhu |
author_sort | Hu, Rui |
collection | PubMed |
description | Acral melanoma is the major subtype of melanoma in Chinese patients. However, a majority of current studies focused on non‐acral melanoma. Most immortalized melanoma cell lines and primary cells were not from acral melanoma. Besides, there are rarely reports about methods for establishing primary acral melanoma cell cultures and related animal models. Here, we present four new human primary acral melanoma cell lines. To determine the mutational profile of the established primary melanoma cells for future targeted use, we performed exome sequencing. We next examined cell proliferation of the primary acral melanoma cells by colony‐formation assays and CCK8 assay. We also evaluated the proliferative and metastatic potential of XYAM‐4 in vivo. We report a detailed protocol for establishing cultured primary acral melanoma cells for Chinese patients and related animal models. We also summarize the features in our acral melanoma cell lines and the existing acral melanoma cell lines. This will provide an effective research tool for research on drug responses and individualized treatment for Chinese patients and comparative studies of melanomas between western and Chinese populations. |
format | Online Article Text |
id | pubmed-9291188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92911882022-07-20 Establishment of cultured primary acral melanoma cells and animal models for Chinese patients Hu, Rui Zhao, Shuang Su, Juan Chen, Xiang Yin, Mingzhu Pigment Cell Melanoma Res Resource and Technologies Acral melanoma is the major subtype of melanoma in Chinese patients. However, a majority of current studies focused on non‐acral melanoma. Most immortalized melanoma cell lines and primary cells were not from acral melanoma. Besides, there are rarely reports about methods for establishing primary acral melanoma cell cultures and related animal models. Here, we present four new human primary acral melanoma cell lines. To determine the mutational profile of the established primary melanoma cells for future targeted use, we performed exome sequencing. We next examined cell proliferation of the primary acral melanoma cells by colony‐formation assays and CCK8 assay. We also evaluated the proliferative and metastatic potential of XYAM‐4 in vivo. We report a detailed protocol for establishing cultured primary acral melanoma cells for Chinese patients and related animal models. We also summarize the features in our acral melanoma cell lines and the existing acral melanoma cell lines. This will provide an effective research tool for research on drug responses and individualized treatment for Chinese patients and comparative studies of melanomas between western and Chinese populations. John Wiley and Sons Inc. 2021-06-28 2021-11 /pmc/articles/PMC9291188/ /pubmed/34097822 http://dx.doi.org/10.1111/pcmr.12996 Text en © 2021 The Authors. Pigment Cell & Melanoma Research published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Resource and Technologies Hu, Rui Zhao, Shuang Su, Juan Chen, Xiang Yin, Mingzhu Establishment of cultured primary acral melanoma cells and animal models for Chinese patients |
title | Establishment of cultured primary acral melanoma cells and animal models for Chinese patients |
title_full | Establishment of cultured primary acral melanoma cells and animal models for Chinese patients |
title_fullStr | Establishment of cultured primary acral melanoma cells and animal models for Chinese patients |
title_full_unstemmed | Establishment of cultured primary acral melanoma cells and animal models for Chinese patients |
title_short | Establishment of cultured primary acral melanoma cells and animal models for Chinese patients |
title_sort | establishment of cultured primary acral melanoma cells and animal models for chinese patients |
topic | Resource and Technologies |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291188/ https://www.ncbi.nlm.nih.gov/pubmed/34097822 http://dx.doi.org/10.1111/pcmr.12996 |
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