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Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes

Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α‐1,6 glycosidic bond between α‐1,4 linked glucooliogosaccharides. As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far. This study aimed at acquiring a better...

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Detalles Bibliográficos
Autores principales: Xiang, Gang, Leemhuis, Hans, van der Maarel, Marc Jos Elise Cornelis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291813/
https://www.ncbi.nlm.nih.gov/pubmed/34346105
http://dx.doi.org/10.1002/prot.26200
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author Xiang, Gang
Leemhuis, Hans
van der Maarel, Marc Jos Elise Cornelis
author_facet Xiang, Gang
Leemhuis, Hans
van der Maarel, Marc Jos Elise Cornelis
author_sort Xiang, Gang
collection PubMed
description Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α‐1,6 glycosidic bond between α‐1,4 linked glucooliogosaccharides. As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far. This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence‐based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs. A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β‐strands.
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spelling pubmed-92918132022-07-20 Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes Xiang, Gang Leemhuis, Hans van der Maarel, Marc Jos Elise Cornelis Proteins Research Articles Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α‐1,6 glycosidic bond between α‐1,4 linked glucooliogosaccharides. As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far. This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence‐based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs. A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β‐strands. John Wiley & Sons, Inc. 2021-08-09 2022-01 /pmc/articles/PMC9291813/ /pubmed/34346105 http://dx.doi.org/10.1002/prot.26200 Text en © 2021 The Authors. Proteins: Structure, Function, and Bioinformatics published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Xiang, Gang
Leemhuis, Hans
van der Maarel, Marc Jos Elise Cornelis
Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes
title Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes
title_full Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes
title_fullStr Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes
title_full_unstemmed Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes
title_short Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes
title_sort structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291813/
https://www.ncbi.nlm.nih.gov/pubmed/34346105
http://dx.doi.org/10.1002/prot.26200
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