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Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes
Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α‐1,6 glycosidic bond between α‐1,4 linked glucooliogosaccharides. As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far. This study aimed at acquiring a better...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291813/ https://www.ncbi.nlm.nih.gov/pubmed/34346105 http://dx.doi.org/10.1002/prot.26200 |
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author | Xiang, Gang Leemhuis, Hans van der Maarel, Marc Jos Elise Cornelis |
author_facet | Xiang, Gang Leemhuis, Hans van der Maarel, Marc Jos Elise Cornelis |
author_sort | Xiang, Gang |
collection | PubMed |
description | Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α‐1,6 glycosidic bond between α‐1,4 linked glucooliogosaccharides. As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far. This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence‐based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs. A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β‐strands. |
format | Online Article Text |
id | pubmed-9291813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92918132022-07-20 Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes Xiang, Gang Leemhuis, Hans van der Maarel, Marc Jos Elise Cornelis Proteins Research Articles Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α‐1,6 glycosidic bond between α‐1,4 linked glucooliogosaccharides. As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far. This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence‐based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs. A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β‐strands. John Wiley & Sons, Inc. 2021-08-09 2022-01 /pmc/articles/PMC9291813/ /pubmed/34346105 http://dx.doi.org/10.1002/prot.26200 Text en © 2021 The Authors. Proteins: Structure, Function, and Bioinformatics published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Xiang, Gang Leemhuis, Hans van der Maarel, Marc Jos Elise Cornelis Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes |
title | Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes |
title_full | Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes |
title_fullStr | Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes |
title_full_unstemmed | Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes |
title_short | Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes |
title_sort | structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291813/ https://www.ncbi.nlm.nih.gov/pubmed/34346105 http://dx.doi.org/10.1002/prot.26200 |
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