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The first chromosome‐level genome assembly of a green lacewing Chrysopa pallens and its implication for biological control

Many lacewing species (Insecta: Neuroptera) are important predators of pests with great potential in biological control. So far, there is no chromosome‐level published genome available for Neuroptera. Here we report a high‐quality chromosome‐level reference genome for a green lacewing species Chryso...

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Autores principales: Wang, Yuyu, Zhang, Ruyue, Wang, Mengqing, Zhang, Lisheng, Shi, Cheng‐Min, Li, Jing, Fan, Fan, Geng, Shuo, Liu, Xingyue, Yang, Ding
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9292380/
https://www.ncbi.nlm.nih.gov/pubmed/34549894
http://dx.doi.org/10.1111/1755-0998.13503
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author Wang, Yuyu
Zhang, Ruyue
Wang, Mengqing
Zhang, Lisheng
Shi, Cheng‐Min
Li, Jing
Fan, Fan
Geng, Shuo
Liu, Xingyue
Yang, Ding
author_facet Wang, Yuyu
Zhang, Ruyue
Wang, Mengqing
Zhang, Lisheng
Shi, Cheng‐Min
Li, Jing
Fan, Fan
Geng, Shuo
Liu, Xingyue
Yang, Ding
author_sort Wang, Yuyu
collection PubMed
description Many lacewing species (Insecta: Neuroptera) are important predators of pests with great potential in biological control. So far, there is no chromosome‐level published genome available for Neuroptera. Here we report a high‐quality chromosome‐level reference genome for a green lacewing species Chrysopa pallens (Neuroptera: Chrysopidae), which is one of the most important insect natural enemies used in pest biocontrol. The genome was sequenced using a combination of PacBio and Hi‐C technologies and assembled into seven chromosomes with a total size of 517.21 Mb, occupying 96.07% of the genome sequence. A total of 12,840 protein‐coding genes were identified and approximately 206.21 Mb of repeated sequences were annotated. Phylogenetic analyses indicated that C. pallens diverged from its common ancestor with Tribolium castaneum (Coleoptera) approximately 300 million years ago. The gene families involved in digestion, detoxification, chemoreception, carbohydrate metabolism, immunity, nerves and development were significantly expanded, revealing the potential genomic basis for the polyphagia of C. pallens and its role as an excellent biocontrol agent. This high‐quality genome of C. pallens will provide an important genomic resource for future population genetics, evolutionary and phylogenetic investigations of Chrysopidae as well as comparative genomic studies of Neuropterida and other insects.
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spelling pubmed-92923802022-07-20 The first chromosome‐level genome assembly of a green lacewing Chrysopa pallens and its implication for biological control Wang, Yuyu Zhang, Ruyue Wang, Mengqing Zhang, Lisheng Shi, Cheng‐Min Li, Jing Fan, Fan Geng, Shuo Liu, Xingyue Yang, Ding Mol Ecol Resour RESOURCE ARTICLES Many lacewing species (Insecta: Neuroptera) are important predators of pests with great potential in biological control. So far, there is no chromosome‐level published genome available for Neuroptera. Here we report a high‐quality chromosome‐level reference genome for a green lacewing species Chrysopa pallens (Neuroptera: Chrysopidae), which is one of the most important insect natural enemies used in pest biocontrol. The genome was sequenced using a combination of PacBio and Hi‐C technologies and assembled into seven chromosomes with a total size of 517.21 Mb, occupying 96.07% of the genome sequence. A total of 12,840 protein‐coding genes were identified and approximately 206.21 Mb of repeated sequences were annotated. Phylogenetic analyses indicated that C. pallens diverged from its common ancestor with Tribolium castaneum (Coleoptera) approximately 300 million years ago. The gene families involved in digestion, detoxification, chemoreception, carbohydrate metabolism, immunity, nerves and development were significantly expanded, revealing the potential genomic basis for the polyphagia of C. pallens and its role as an excellent biocontrol agent. This high‐quality genome of C. pallens will provide an important genomic resource for future population genetics, evolutionary and phylogenetic investigations of Chrysopidae as well as comparative genomic studies of Neuropterida and other insects. John Wiley and Sons Inc. 2021-09-30 2022-02 /pmc/articles/PMC9292380/ /pubmed/34549894 http://dx.doi.org/10.1111/1755-0998.13503 Text en © 2021 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle RESOURCE ARTICLES
Wang, Yuyu
Zhang, Ruyue
Wang, Mengqing
Zhang, Lisheng
Shi, Cheng‐Min
Li, Jing
Fan, Fan
Geng, Shuo
Liu, Xingyue
Yang, Ding
The first chromosome‐level genome assembly of a green lacewing Chrysopa pallens and its implication for biological control
title The first chromosome‐level genome assembly of a green lacewing Chrysopa pallens and its implication for biological control
title_full The first chromosome‐level genome assembly of a green lacewing Chrysopa pallens and its implication for biological control
title_fullStr The first chromosome‐level genome assembly of a green lacewing Chrysopa pallens and its implication for biological control
title_full_unstemmed The first chromosome‐level genome assembly of a green lacewing Chrysopa pallens and its implication for biological control
title_short The first chromosome‐level genome assembly of a green lacewing Chrysopa pallens and its implication for biological control
title_sort first chromosome‐level genome assembly of a green lacewing chrysopa pallens and its implication for biological control
topic RESOURCE ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9292380/
https://www.ncbi.nlm.nih.gov/pubmed/34549894
http://dx.doi.org/10.1111/1755-0998.13503
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