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Correlative imaging using super‐resolution fluorescence microscopy and soft X‐ray tomography at cryogenic temperatures provides a new way to assess virosome solutions for vaccine development
Active virosomes (AVs) are derivatives of viruses, broadly similar to ‘parent’ pathogens, with an outer envelope that contains a bespoke genome coding for four to five viral proteins capable of eliciting an antigenic response. AVs are essentially novel vaccine formulations that present on their surf...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9292697/ https://www.ncbi.nlm.nih.gov/pubmed/34333776 http://dx.doi.org/10.1111/jmi.13054 |
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author | Okolo, Chidinma A. Jadhav, Archana Phillips, Patrick Dumoux, Maud McMurray, Amanda A. Joshi, Vishwas D. Pizzey, Claire Harkiolaki, Maria |
author_facet | Okolo, Chidinma A. Jadhav, Archana Phillips, Patrick Dumoux, Maud McMurray, Amanda A. Joshi, Vishwas D. Pizzey, Claire Harkiolaki, Maria |
author_sort | Okolo, Chidinma A. |
collection | PubMed |
description | Active virosomes (AVs) are derivatives of viruses, broadly similar to ‘parent’ pathogens, with an outer envelope that contains a bespoke genome coding for four to five viral proteins capable of eliciting an antigenic response. AVs are essentially novel vaccine formulations that present on their surface selected viral proteins as antigens. Once administered, they elicit an initial ‘anti‐viral’ immune response. AVs are also internalised by host cells where their cargo viral genes are used to express viral antigen(s) intracellularly. These can then be transported to the host cell surface resulting in a second wave of antigen exposure and a more potent immuno‐stimulation. A new 3D correlative microscopy approach is used here to provide a robust analytical method for characterisation of Zika‐ and Chikungunya‐derivatised AV populations including vesicle size distribution and variations in antigen loading. Manufactured batches were compared to assess the extent and nature of batch‐to‐batch variations. We also show preliminary results that verify antigen expression on the surface of host cells. We present here a reliable and efficient high‐resolution 3D imaging regime that allows the evaluation of the microstructure and biochemistry of novel vaccine formulations such as AVs. |
format | Online Article Text |
id | pubmed-9292697 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92926972022-07-20 Correlative imaging using super‐resolution fluorescence microscopy and soft X‐ray tomography at cryogenic temperatures provides a new way to assess virosome solutions for vaccine development Okolo, Chidinma A. Jadhav, Archana Phillips, Patrick Dumoux, Maud McMurray, Amanda A. Joshi, Vishwas D. Pizzey, Claire Harkiolaki, Maria J Microsc Original Articles Active virosomes (AVs) are derivatives of viruses, broadly similar to ‘parent’ pathogens, with an outer envelope that contains a bespoke genome coding for four to five viral proteins capable of eliciting an antigenic response. AVs are essentially novel vaccine formulations that present on their surface selected viral proteins as antigens. Once administered, they elicit an initial ‘anti‐viral’ immune response. AVs are also internalised by host cells where their cargo viral genes are used to express viral antigen(s) intracellularly. These can then be transported to the host cell surface resulting in a second wave of antigen exposure and a more potent immuno‐stimulation. A new 3D correlative microscopy approach is used here to provide a robust analytical method for characterisation of Zika‐ and Chikungunya‐derivatised AV populations including vesicle size distribution and variations in antigen loading. Manufactured batches were compared to assess the extent and nature of batch‐to‐batch variations. We also show preliminary results that verify antigen expression on the surface of host cells. We present here a reliable and efficient high‐resolution 3D imaging regime that allows the evaluation of the microstructure and biochemistry of novel vaccine formulations such as AVs. John Wiley and Sons Inc. 2021-09-03 2021-12 /pmc/articles/PMC9292697/ /pubmed/34333776 http://dx.doi.org/10.1111/jmi.13054 Text en © 2021 The Authors. Journal of Microscopy published by John Wiley & Sons Ltd on behalf of Royal Microscopical Society https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Okolo, Chidinma A. Jadhav, Archana Phillips, Patrick Dumoux, Maud McMurray, Amanda A. Joshi, Vishwas D. Pizzey, Claire Harkiolaki, Maria Correlative imaging using super‐resolution fluorescence microscopy and soft X‐ray tomography at cryogenic temperatures provides a new way to assess virosome solutions for vaccine development |
title | Correlative imaging using super‐resolution fluorescence microscopy and soft X‐ray tomography at cryogenic temperatures provides a new way to assess virosome solutions for vaccine development |
title_full | Correlative imaging using super‐resolution fluorescence microscopy and soft X‐ray tomography at cryogenic temperatures provides a new way to assess virosome solutions for vaccine development |
title_fullStr | Correlative imaging using super‐resolution fluorescence microscopy and soft X‐ray tomography at cryogenic temperatures provides a new way to assess virosome solutions for vaccine development |
title_full_unstemmed | Correlative imaging using super‐resolution fluorescence microscopy and soft X‐ray tomography at cryogenic temperatures provides a new way to assess virosome solutions for vaccine development |
title_short | Correlative imaging using super‐resolution fluorescence microscopy and soft X‐ray tomography at cryogenic temperatures provides a new way to assess virosome solutions for vaccine development |
title_sort | correlative imaging using super‐resolution fluorescence microscopy and soft x‐ray tomography at cryogenic temperatures provides a new way to assess virosome solutions for vaccine development |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9292697/ https://www.ncbi.nlm.nih.gov/pubmed/34333776 http://dx.doi.org/10.1111/jmi.13054 |
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