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ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels

Maize kernel weight is influenced by the unloading of nutrients from the maternal placenta and their passage through the transfer tissue of the basal endosperm transfer layer (BETL) and the basal intermediate zone (BIZ) to the upper part of the endosperm. Here, we show that Small kernel 10 (Smk10) e...

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Autores principales: Hu, Mingjian, Zhao, Haiming, Yang, Bo, Yang, Shuang, Liu, Haihong, Tian, He, Shui, Guanghou, Chen, Zongliang, E, Lizhu, Lai, Jinsheng, Song, Weibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9292782/
https://www.ncbi.nlm.nih.gov/pubmed/34559890
http://dx.doi.org/10.1111/nph.17754
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author Hu, Mingjian
Zhao, Haiming
Yang, Bo
Yang, Shuang
Liu, Haihong
Tian, He
Shui, Guanghou
Chen, Zongliang
E, Lizhu
Lai, Jinsheng
Song, Weibin
author_facet Hu, Mingjian
Zhao, Haiming
Yang, Bo
Yang, Shuang
Liu, Haihong
Tian, He
Shui, Guanghou
Chen, Zongliang
E, Lizhu
Lai, Jinsheng
Song, Weibin
author_sort Hu, Mingjian
collection PubMed
description Maize kernel weight is influenced by the unloading of nutrients from the maternal placenta and their passage through the transfer tissue of the basal endosperm transfer layer (BETL) and the basal intermediate zone (BIZ) to the upper part of the endosperm. Here, we show that Small kernel 10 (Smk10) encodes a choline transporter‐like protein 1 (ZmCTLP1) that facilitates choline uptake and is located in the trans‐Golgi network (TGN). Its loss of function results in reduced choline content, leading to smaller kernels with a lower starch content. Mutation of ZmCTLP1 disrupts membrane lipid homeostasis and the normal development of wall in‐growths. Expression levels of Mn1 and ZmSWEET4c, two kernel filling‐related genes, are downregulated in the smk10, which is likely to be one of the major causes of incompletely differentiated transfer cells. Mutation of ZmCTLP1 also reduces the number of plasmodesmata (PD) in transfer cells, indicating that the smk10 mutant is impaired in PD formation. Intriguingly, we also observed premature cell death in the BETL and BIZ of the smk10 mutant. Together, our results suggest that ZmCTLP1‐mediated choline transport affects kernel development, highlighting its important role in lipid homeostasis, wall in‐growth formation and PD development in transfer cells.
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spelling pubmed-92927822022-07-20 ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels Hu, Mingjian Zhao, Haiming Yang, Bo Yang, Shuang Liu, Haihong Tian, He Shui, Guanghou Chen, Zongliang E, Lizhu Lai, Jinsheng Song, Weibin New Phytol Research Maize kernel weight is influenced by the unloading of nutrients from the maternal placenta and their passage through the transfer tissue of the basal endosperm transfer layer (BETL) and the basal intermediate zone (BIZ) to the upper part of the endosperm. Here, we show that Small kernel 10 (Smk10) encodes a choline transporter‐like protein 1 (ZmCTLP1) that facilitates choline uptake and is located in the trans‐Golgi network (TGN). Its loss of function results in reduced choline content, leading to smaller kernels with a lower starch content. Mutation of ZmCTLP1 disrupts membrane lipid homeostasis and the normal development of wall in‐growths. Expression levels of Mn1 and ZmSWEET4c, two kernel filling‐related genes, are downregulated in the smk10, which is likely to be one of the major causes of incompletely differentiated transfer cells. Mutation of ZmCTLP1 also reduces the number of plasmodesmata (PD) in transfer cells, indicating that the smk10 mutant is impaired in PD formation. Intriguingly, we also observed premature cell death in the BETL and BIZ of the smk10 mutant. Together, our results suggest that ZmCTLP1‐mediated choline transport affects kernel development, highlighting its important role in lipid homeostasis, wall in‐growth formation and PD development in transfer cells. John Wiley and Sons Inc. 2021-10-11 2021-12 /pmc/articles/PMC9292782/ /pubmed/34559890 http://dx.doi.org/10.1111/nph.17754 Text en © 2021 The Authors. New Phytologist published by John Wiley & Sons Ltd on behalf of New Phytologist Foundation https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Hu, Mingjian
Zhao, Haiming
Yang, Bo
Yang, Shuang
Liu, Haihong
Tian, He
Shui, Guanghou
Chen, Zongliang
E, Lizhu
Lai, Jinsheng
Song, Weibin
ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels
title ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels
title_full ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels
title_fullStr ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels
title_full_unstemmed ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels
title_short ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels
title_sort zmctlp1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9292782/
https://www.ncbi.nlm.nih.gov/pubmed/34559890
http://dx.doi.org/10.1111/nph.17754
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