Cargando…

Determination of colistin in luminal and parietal intestinal matrices of chicken by ultra‐high‐performance liquid chromatography‐tandem mass spectrometry

Justification for continued use of colistin in veterinary medicine, for example medicated water, relies on pharmacokinetic/pharmacodynamic (PK/PD) studies that require accurate measurement of colistin content in the digestive tract. A method for the detection and quantification of colistin in poultr...

Descripción completa

Detalles Bibliográficos
Autores principales: Mead, Andrew, Gillard, Nathalie, Robert, Christelle, Pierret, Gilles, Henrottin, Jean, Richez, Pascal, Pelligand, Ludovic
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293311/
https://www.ncbi.nlm.nih.gov/pubmed/34614207
http://dx.doi.org/10.1111/jvp.13022
_version_ 1784749597821763584
author Mead, Andrew
Gillard, Nathalie
Robert, Christelle
Pierret, Gilles
Henrottin, Jean
Richez, Pascal
Pelligand, Ludovic
author_facet Mead, Andrew
Gillard, Nathalie
Robert, Christelle
Pierret, Gilles
Henrottin, Jean
Richez, Pascal
Pelligand, Ludovic
author_sort Mead, Andrew
collection PubMed
description Justification for continued use of colistin in veterinary medicine, for example medicated water, relies on pharmacokinetic/pharmacodynamic (PK/PD) studies that require accurate measurement of colistin content in the digestive tract. A method for the detection and quantification of colistin in poultry intestinal material was developed and validated. Colistin is not absorbed after oral administration, and the biophase is the gastrointestinal tract. Extraction of colistin from the matrix was achieved using solid‐phase extraction with a methanol:water (1:2; v/v) solution. Polymyxin B was used as an internal standard. Colistin A and colistin B, the main components of colistin, were separated, detected and measured using ultra‐high‐performance liquid chromatography coupled with tandem mass spectrometry (UHPLC‐MS/MS). The method was validated for linearity/quadraticity between 1.1 (LOQ) and 56.7 mg/kg. Mean accuracy was between 82.7% and 107.7% with inter‐ and intra‐day precision lower than 13.3% and 15% respectively. Freeze–thaw, long‐term and bench storage were validated. Incurred samples following colistin treatment in poultry at the approved clinical dose of 75,000 IU/kg in drinking water and oral gavage were quantifiable and in line with expected intestinal transit times. The method is considered appropriately accurate and precise for the purposes of pharmacokinetic analysis in the gastrointestinal tract.
format Online
Article
Text
id pubmed-9293311
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-92933112022-07-20 Determination of colistin in luminal and parietal intestinal matrices of chicken by ultra‐high‐performance liquid chromatography‐tandem mass spectrometry Mead, Andrew Gillard, Nathalie Robert, Christelle Pierret, Gilles Henrottin, Jean Richez, Pascal Pelligand, Ludovic J Vet Pharmacol Ther Short Communication Justification for continued use of colistin in veterinary medicine, for example medicated water, relies on pharmacokinetic/pharmacodynamic (PK/PD) studies that require accurate measurement of colistin content in the digestive tract. A method for the detection and quantification of colistin in poultry intestinal material was developed and validated. Colistin is not absorbed after oral administration, and the biophase is the gastrointestinal tract. Extraction of colistin from the matrix was achieved using solid‐phase extraction with a methanol:water (1:2; v/v) solution. Polymyxin B was used as an internal standard. Colistin A and colistin B, the main components of colistin, were separated, detected and measured using ultra‐high‐performance liquid chromatography coupled with tandem mass spectrometry (UHPLC‐MS/MS). The method was validated for linearity/quadraticity between 1.1 (LOQ) and 56.7 mg/kg. Mean accuracy was between 82.7% and 107.7% with inter‐ and intra‐day precision lower than 13.3% and 15% respectively. Freeze–thaw, long‐term and bench storage were validated. Incurred samples following colistin treatment in poultry at the approved clinical dose of 75,000 IU/kg in drinking water and oral gavage were quantifiable and in line with expected intestinal transit times. The method is considered appropriately accurate and precise for the purposes of pharmacokinetic analysis in the gastrointestinal tract. John Wiley and Sons Inc. 2021-10-06 2021-11 /pmc/articles/PMC9293311/ /pubmed/34614207 http://dx.doi.org/10.1111/jvp.13022 Text en © 2021 The Authors. Journal of Veterinary Pharmacology and Therapeutics published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Mead, Andrew
Gillard, Nathalie
Robert, Christelle
Pierret, Gilles
Henrottin, Jean
Richez, Pascal
Pelligand, Ludovic
Determination of colistin in luminal and parietal intestinal matrices of chicken by ultra‐high‐performance liquid chromatography‐tandem mass spectrometry
title Determination of colistin in luminal and parietal intestinal matrices of chicken by ultra‐high‐performance liquid chromatography‐tandem mass spectrometry
title_full Determination of colistin in luminal and parietal intestinal matrices of chicken by ultra‐high‐performance liquid chromatography‐tandem mass spectrometry
title_fullStr Determination of colistin in luminal and parietal intestinal matrices of chicken by ultra‐high‐performance liquid chromatography‐tandem mass spectrometry
title_full_unstemmed Determination of colistin in luminal and parietal intestinal matrices of chicken by ultra‐high‐performance liquid chromatography‐tandem mass spectrometry
title_short Determination of colistin in luminal and parietal intestinal matrices of chicken by ultra‐high‐performance liquid chromatography‐tandem mass spectrometry
title_sort determination of colistin in luminal and parietal intestinal matrices of chicken by ultra‐high‐performance liquid chromatography‐tandem mass spectrometry
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293311/
https://www.ncbi.nlm.nih.gov/pubmed/34614207
http://dx.doi.org/10.1111/jvp.13022
work_keys_str_mv AT meadandrew determinationofcolistininluminalandparietalintestinalmatricesofchickenbyultrahighperformanceliquidchromatographytandemmassspectrometry
AT gillardnathalie determinationofcolistininluminalandparietalintestinalmatricesofchickenbyultrahighperformanceliquidchromatographytandemmassspectrometry
AT robertchristelle determinationofcolistininluminalandparietalintestinalmatricesofchickenbyultrahighperformanceliquidchromatographytandemmassspectrometry
AT pierretgilles determinationofcolistininluminalandparietalintestinalmatricesofchickenbyultrahighperformanceliquidchromatographytandemmassspectrometry
AT henrottinjean determinationofcolistininluminalandparietalintestinalmatricesofchickenbyultrahighperformanceliquidchromatographytandemmassspectrometry
AT richezpascal determinationofcolistininluminalandparietalintestinalmatricesofchickenbyultrahighperformanceliquidchromatographytandemmassspectrometry
AT pelligandludovic determinationofcolistininluminalandparietalintestinalmatricesofchickenbyultrahighperformanceliquidchromatographytandemmassspectrometry