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Introgression of bruchid ( Zabrotes subfasciatus) resistance into small red common bean ( Phaseolus vulgaris ) background and validation of the BRU_00261 (snpPV0007) resistance marker
Bruchids are a major storage pest of common bean. Genetic resistance is a suitable method to avoid grain losses during storage. The objective of the study was to introgress the arcelin‐based resistance locus into selected advanced breeding line and to validate the molecular marker BRU_00261. A total...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293403/ https://www.ncbi.nlm.nih.gov/pubmed/35875723 http://dx.doi.org/10.1111/pbr.12969 |
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author | Tigist, Shiferaw Girsil Raatz, Bodo Assefa, Amelework Melis, Rob Sibiya, Julia Keneni, Gemechu Mukankusi, Clare Fenta, Berhanu Ketema, Selamawit Tsegaye, Dagmawit |
author_facet | Tigist, Shiferaw Girsil Raatz, Bodo Assefa, Amelework Melis, Rob Sibiya, Julia Keneni, Gemechu Mukankusi, Clare Fenta, Berhanu Ketema, Selamawit Tsegaye, Dagmawit |
author_sort | Tigist, Shiferaw Girsil |
collection | PubMed |
description | Bruchids are a major storage pest of common bean. Genetic resistance is a suitable method to avoid grain losses during storage. The objective of the study was to introgress the arcelin‐based resistance locus into selected advanced breeding line and to validate the molecular marker BRU_00261. A total of 208 progeny F(4) families were phenotyped using a randomized complete block design, with three replications. Highly significant differences (P < .001) among the entries, parents and offspring were recorded for almost all traits. There was no significant difference between the two parents in the number of eggs laid. The progenies were grouped as highly resistant (34.3%), resistant (11.9%), moderately resistant (21.4%) and susceptible (32.4%). The levels of broad sense heritability ranged from 68.5%–93.9% for all the traits. Eighty‐three most resistant lines and the parental lines were genotyped with the marker BRU_00261 (snpPV0007). The marker segregation deviated significantly from the expected independent segregation towards a strong enrichment for the resistant marker in the selected families. This marker will be useful for selecting promising materials in early generations and phenotypic confirmation of positive lines in later generations. |
format | Online Article Text |
id | pubmed-9293403 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92934032022-07-20 Introgression of bruchid ( Zabrotes subfasciatus) resistance into small red common bean ( Phaseolus vulgaris ) background and validation of the BRU_00261 (snpPV0007) resistance marker Tigist, Shiferaw Girsil Raatz, Bodo Assefa, Amelework Melis, Rob Sibiya, Julia Keneni, Gemechu Mukankusi, Clare Fenta, Berhanu Ketema, Selamawit Tsegaye, Dagmawit Plant Breed Original Articles Bruchids are a major storage pest of common bean. Genetic resistance is a suitable method to avoid grain losses during storage. The objective of the study was to introgress the arcelin‐based resistance locus into selected advanced breeding line and to validate the molecular marker BRU_00261. A total of 208 progeny F(4) families were phenotyped using a randomized complete block design, with three replications. Highly significant differences (P < .001) among the entries, parents and offspring were recorded for almost all traits. There was no significant difference between the two parents in the number of eggs laid. The progenies were grouped as highly resistant (34.3%), resistant (11.9%), moderately resistant (21.4%) and susceptible (32.4%). The levels of broad sense heritability ranged from 68.5%–93.9% for all the traits. Eighty‐three most resistant lines and the parental lines were genotyped with the marker BRU_00261 (snpPV0007). The marker segregation deviated significantly from the expected independent segregation towards a strong enrichment for the resistant marker in the selected families. This marker will be useful for selecting promising materials in early generations and phenotypic confirmation of positive lines in later generations. John Wiley and Sons Inc. 2021-10-09 2021-12 /pmc/articles/PMC9293403/ /pubmed/35875723 http://dx.doi.org/10.1111/pbr.12969 Text en © 2021 The Authors. Plant Breeding published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Tigist, Shiferaw Girsil Raatz, Bodo Assefa, Amelework Melis, Rob Sibiya, Julia Keneni, Gemechu Mukankusi, Clare Fenta, Berhanu Ketema, Selamawit Tsegaye, Dagmawit Introgression of bruchid ( Zabrotes subfasciatus) resistance into small red common bean ( Phaseolus vulgaris ) background and validation of the BRU_00261 (snpPV0007) resistance marker |
title | Introgression of bruchid (
Zabrotes subfasciatus) resistance into small red common bean (
Phaseolus vulgaris
) background and validation of the BRU_00261 (snpPV0007) resistance marker |
title_full | Introgression of bruchid (
Zabrotes subfasciatus) resistance into small red common bean (
Phaseolus vulgaris
) background and validation of the BRU_00261 (snpPV0007) resistance marker |
title_fullStr | Introgression of bruchid (
Zabrotes subfasciatus) resistance into small red common bean (
Phaseolus vulgaris
) background and validation of the BRU_00261 (snpPV0007) resistance marker |
title_full_unstemmed | Introgression of bruchid (
Zabrotes subfasciatus) resistance into small red common bean (
Phaseolus vulgaris
) background and validation of the BRU_00261 (snpPV0007) resistance marker |
title_short | Introgression of bruchid (
Zabrotes subfasciatus) resistance into small red common bean (
Phaseolus vulgaris
) background and validation of the BRU_00261 (snpPV0007) resistance marker |
title_sort | introgression of bruchid (
zabrotes subfasciatus) resistance into small red common bean (
phaseolus vulgaris
) background and validation of the bru_00261 (snppv0007) resistance marker |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293403/ https://www.ncbi.nlm.nih.gov/pubmed/35875723 http://dx.doi.org/10.1111/pbr.12969 |
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