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A toolbox for conditional control of gene expression in apicomplexan parasites
Apicomplexan parasites encompass diverse pathogens for humans and animals, including the causative agents of malaria and toxoplasmosis, Plasmodium spp. and Toxoplasma gondii. Genetic manipulation of these parasites has become central to explore parasite biology, unravel gene function and identify ne...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293482/ https://www.ncbi.nlm.nih.gov/pubmed/34564906 http://dx.doi.org/10.1111/mmi.14821 |
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author | Briquet, Sylvie Gissot, Mathieu Silvie, Olivier |
author_facet | Briquet, Sylvie Gissot, Mathieu Silvie, Olivier |
author_sort | Briquet, Sylvie |
collection | PubMed |
description | Apicomplexan parasites encompass diverse pathogens for humans and animals, including the causative agents of malaria and toxoplasmosis, Plasmodium spp. and Toxoplasma gondii. Genetic manipulation of these parasites has become central to explore parasite biology, unravel gene function and identify new targets for therapeutic strategies. Tremendous progress has been achieved over the past years with the advent of next generation sequencing and powerful genome editing methods. In particular, various methods for conditional gene expression have been developed in both Plasmodium and Toxoplasma to knockout or knockdown essential genes, or for inducible expression of master developmental regulators or mutant versions of proteins. Conditional gene expression can be achieved at three distinct levels. At the DNA level, inducible site‐specific recombinases allow conditional genome editing. At the RNA level, regulation can be achieved during transcription, using stage‐specific or regulatable promoters, or post‐transcriptionally through alteration of mRNA stability or translation. At the protein level, several systems have been developed for inducible degradation or displacement of a protein of interest. In this review, we provide an overview of current systems for conditional control of gene expression in Plasmodium and Toxoplasma parasites, highlighting the advantages and limitations of each approach. |
format | Online Article Text |
id | pubmed-9293482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92934822022-07-20 A toolbox for conditional control of gene expression in apicomplexan parasites Briquet, Sylvie Gissot, Mathieu Silvie, Olivier Mol Microbiol Microreviews Apicomplexan parasites encompass diverse pathogens for humans and animals, including the causative agents of malaria and toxoplasmosis, Plasmodium spp. and Toxoplasma gondii. Genetic manipulation of these parasites has become central to explore parasite biology, unravel gene function and identify new targets for therapeutic strategies. Tremendous progress has been achieved over the past years with the advent of next generation sequencing and powerful genome editing methods. In particular, various methods for conditional gene expression have been developed in both Plasmodium and Toxoplasma to knockout or knockdown essential genes, or for inducible expression of master developmental regulators or mutant versions of proteins. Conditional gene expression can be achieved at three distinct levels. At the DNA level, inducible site‐specific recombinases allow conditional genome editing. At the RNA level, regulation can be achieved during transcription, using stage‐specific or regulatable promoters, or post‐transcriptionally through alteration of mRNA stability or translation. At the protein level, several systems have been developed for inducible degradation or displacement of a protein of interest. In this review, we provide an overview of current systems for conditional control of gene expression in Plasmodium and Toxoplasma parasites, highlighting the advantages and limitations of each approach. John Wiley and Sons Inc. 2021-10-13 2022-03 /pmc/articles/PMC9293482/ /pubmed/34564906 http://dx.doi.org/10.1111/mmi.14821 Text en © 2021 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Microreviews Briquet, Sylvie Gissot, Mathieu Silvie, Olivier A toolbox for conditional control of gene expression in apicomplexan parasites |
title | A toolbox for conditional control of gene expression in apicomplexan parasites |
title_full | A toolbox for conditional control of gene expression in apicomplexan parasites |
title_fullStr | A toolbox for conditional control of gene expression in apicomplexan parasites |
title_full_unstemmed | A toolbox for conditional control of gene expression in apicomplexan parasites |
title_short | A toolbox for conditional control of gene expression in apicomplexan parasites |
title_sort | toolbox for conditional control of gene expression in apicomplexan parasites |
topic | Microreviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293482/ https://www.ncbi.nlm.nih.gov/pubmed/34564906 http://dx.doi.org/10.1111/mmi.14821 |
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