Cargando…

A toolbox for conditional control of gene expression in apicomplexan parasites

Apicomplexan parasites encompass diverse pathogens for humans and animals, including the causative agents of malaria and toxoplasmosis, Plasmodium spp. and Toxoplasma gondii. Genetic manipulation of these parasites has become central to explore parasite biology, unravel gene function and identify ne...

Descripción completa

Detalles Bibliográficos
Autores principales: Briquet, Sylvie, Gissot, Mathieu, Silvie, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293482/
https://www.ncbi.nlm.nih.gov/pubmed/34564906
http://dx.doi.org/10.1111/mmi.14821
_version_ 1784749642860199936
author Briquet, Sylvie
Gissot, Mathieu
Silvie, Olivier
author_facet Briquet, Sylvie
Gissot, Mathieu
Silvie, Olivier
author_sort Briquet, Sylvie
collection PubMed
description Apicomplexan parasites encompass diverse pathogens for humans and animals, including the causative agents of malaria and toxoplasmosis, Plasmodium spp. and Toxoplasma gondii. Genetic manipulation of these parasites has become central to explore parasite biology, unravel gene function and identify new targets for therapeutic strategies. Tremendous progress has been achieved over the past years with the advent of next generation sequencing and powerful genome editing methods. In particular, various methods for conditional gene expression have been developed in both Plasmodium and Toxoplasma to knockout or knockdown essential genes, or for inducible expression of master developmental regulators or mutant versions of proteins. Conditional gene expression can be achieved at three distinct levels. At the DNA level, inducible site‐specific recombinases allow conditional genome editing. At the RNA level, regulation can be achieved during transcription, using stage‐specific or regulatable promoters, or post‐transcriptionally through alteration of mRNA stability or translation. At the protein level, several systems have been developed for inducible degradation or displacement of a protein of interest. In this review, we provide an overview of current systems for conditional control of gene expression in Plasmodium and Toxoplasma parasites, highlighting the advantages and limitations of each approach.
format Online
Article
Text
id pubmed-9293482
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-92934822022-07-20 A toolbox for conditional control of gene expression in apicomplexan parasites Briquet, Sylvie Gissot, Mathieu Silvie, Olivier Mol Microbiol Microreviews Apicomplexan parasites encompass diverse pathogens for humans and animals, including the causative agents of malaria and toxoplasmosis, Plasmodium spp. and Toxoplasma gondii. Genetic manipulation of these parasites has become central to explore parasite biology, unravel gene function and identify new targets for therapeutic strategies. Tremendous progress has been achieved over the past years with the advent of next generation sequencing and powerful genome editing methods. In particular, various methods for conditional gene expression have been developed in both Plasmodium and Toxoplasma to knockout or knockdown essential genes, or for inducible expression of master developmental regulators or mutant versions of proteins. Conditional gene expression can be achieved at three distinct levels. At the DNA level, inducible site‐specific recombinases allow conditional genome editing. At the RNA level, regulation can be achieved during transcription, using stage‐specific or regulatable promoters, or post‐transcriptionally through alteration of mRNA stability or translation. At the protein level, several systems have been developed for inducible degradation or displacement of a protein of interest. In this review, we provide an overview of current systems for conditional control of gene expression in Plasmodium and Toxoplasma parasites, highlighting the advantages and limitations of each approach. John Wiley and Sons Inc. 2021-10-13 2022-03 /pmc/articles/PMC9293482/ /pubmed/34564906 http://dx.doi.org/10.1111/mmi.14821 Text en © 2021 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Microreviews
Briquet, Sylvie
Gissot, Mathieu
Silvie, Olivier
A toolbox for conditional control of gene expression in apicomplexan parasites
title A toolbox for conditional control of gene expression in apicomplexan parasites
title_full A toolbox for conditional control of gene expression in apicomplexan parasites
title_fullStr A toolbox for conditional control of gene expression in apicomplexan parasites
title_full_unstemmed A toolbox for conditional control of gene expression in apicomplexan parasites
title_short A toolbox for conditional control of gene expression in apicomplexan parasites
title_sort toolbox for conditional control of gene expression in apicomplexan parasites
topic Microreviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293482/
https://www.ncbi.nlm.nih.gov/pubmed/34564906
http://dx.doi.org/10.1111/mmi.14821
work_keys_str_mv AT briquetsylvie atoolboxforconditionalcontrolofgeneexpressioninapicomplexanparasites
AT gissotmathieu atoolboxforconditionalcontrolofgeneexpressioninapicomplexanparasites
AT silvieolivier atoolboxforconditionalcontrolofgeneexpressioninapicomplexanparasites
AT briquetsylvie toolboxforconditionalcontrolofgeneexpressioninapicomplexanparasites
AT gissotmathieu toolboxforconditionalcontrolofgeneexpressioninapicomplexanparasites
AT silvieolivier toolboxforconditionalcontrolofgeneexpressioninapicomplexanparasites