Cargando…
Antioxidation Function of EGCG by Activating Nrf2/HO-1 Pathway in Mice with Coronary Heart Disease
OBJECTIVE: To explore the effect and mechanism of epigallocatechin gallate (EGCG) in mice with coronary heart disease (CHD). METHODS: Firstly, a CHD model of mouse was established by feeding mice high-fat diet and randomly divided into four groups, including Model group (0.5% sodium cholate) and 10 ...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293516/ https://www.ncbi.nlm.nih.gov/pubmed/35919501 http://dx.doi.org/10.1155/2022/8639139 |
_version_ | 1784749651159678976 |
---|---|
author | Huang, Xiaoyi Chu, Yang Ren, Hua Pang, Xiaofen |
author_facet | Huang, Xiaoyi Chu, Yang Ren, Hua Pang, Xiaofen |
author_sort | Huang, Xiaoyi |
collection | PubMed |
description | OBJECTIVE: To explore the effect and mechanism of epigallocatechin gallate (EGCG) in mice with coronary heart disease (CHD). METHODS: Firstly, a CHD model of mouse was established by feeding mice high-fat diet and randomly divided into four groups, including Model group (0.5% sodium cholate) and 10 mg/kg EGCG, 20 mg/kg EGCG, and 40 mg/kg EGCG groups. After oral administration of sodium cholate or EGCG, HE staining was conducted to assess the pathological changes of mouse cardiac tissues in each group of mice, biochemical kits to measure the levels of blood lipid and oxidative stress substance activity, and western blot to detect matrix metalloproteinase 2 (MMP-2), vascular endothelial growth factor (VEGFA), as well as expression levels of protein related to Nrf2/HO-1/NQO1 pathway in cardiac tissues. RESULTS: The mice in the CHD model appeared to have myocardial pathological damage with elevated serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and decreased high-density lipoprotein cholesterol (HDL-C). Of note, administration of EGCG significantly attenuated myocardial injuries and improved blood lipid levels in mice in a concentration-dependent manner. The advent of EGCG significantly decreased the expression of VEGFA and MMP-2 and increased the activity of superoxide dismutase (SOD), when reducing the content of reactive oxygen species (ROS) in the myocardial tissue and upregulating the expression of HO-1, NQO1, and Nrf2. CONCLUSION: EGCG may reduce atherosclerotic plaque and alleviate pathological damage in the cardiac tissue of CHD mice as well as improve blood lipid levels with antioxidative effect. The mechanism of its effect may be related to the activation of the Nrf2/HO-1/NQO1 antioxidant pathway in vivo of the CHD mice. |
format | Online Article Text |
id | pubmed-9293516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-92935162022-08-01 Antioxidation Function of EGCG by Activating Nrf2/HO-1 Pathway in Mice with Coronary Heart Disease Huang, Xiaoyi Chu, Yang Ren, Hua Pang, Xiaofen Contrast Media Mol Imaging Research Article OBJECTIVE: To explore the effect and mechanism of epigallocatechin gallate (EGCG) in mice with coronary heart disease (CHD). METHODS: Firstly, a CHD model of mouse was established by feeding mice high-fat diet and randomly divided into four groups, including Model group (0.5% sodium cholate) and 10 mg/kg EGCG, 20 mg/kg EGCG, and 40 mg/kg EGCG groups. After oral administration of sodium cholate or EGCG, HE staining was conducted to assess the pathological changes of mouse cardiac tissues in each group of mice, biochemical kits to measure the levels of blood lipid and oxidative stress substance activity, and western blot to detect matrix metalloproteinase 2 (MMP-2), vascular endothelial growth factor (VEGFA), as well as expression levels of protein related to Nrf2/HO-1/NQO1 pathway in cardiac tissues. RESULTS: The mice in the CHD model appeared to have myocardial pathological damage with elevated serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and decreased high-density lipoprotein cholesterol (HDL-C). Of note, administration of EGCG significantly attenuated myocardial injuries and improved blood lipid levels in mice in a concentration-dependent manner. The advent of EGCG significantly decreased the expression of VEGFA and MMP-2 and increased the activity of superoxide dismutase (SOD), when reducing the content of reactive oxygen species (ROS) in the myocardial tissue and upregulating the expression of HO-1, NQO1, and Nrf2. CONCLUSION: EGCG may reduce atherosclerotic plaque and alleviate pathological damage in the cardiac tissue of CHD mice as well as improve blood lipid levels with antioxidative effect. The mechanism of its effect may be related to the activation of the Nrf2/HO-1/NQO1 antioxidant pathway in vivo of the CHD mice. Hindawi 2022-07-11 /pmc/articles/PMC9293516/ /pubmed/35919501 http://dx.doi.org/10.1155/2022/8639139 Text en Copyright © 2022 Xiaoyi Huang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Huang, Xiaoyi Chu, Yang Ren, Hua Pang, Xiaofen Antioxidation Function of EGCG by Activating Nrf2/HO-1 Pathway in Mice with Coronary Heart Disease |
title | Antioxidation Function of EGCG by Activating Nrf2/HO-1 Pathway in Mice with Coronary Heart Disease |
title_full | Antioxidation Function of EGCG by Activating Nrf2/HO-1 Pathway in Mice with Coronary Heart Disease |
title_fullStr | Antioxidation Function of EGCG by Activating Nrf2/HO-1 Pathway in Mice with Coronary Heart Disease |
title_full_unstemmed | Antioxidation Function of EGCG by Activating Nrf2/HO-1 Pathway in Mice with Coronary Heart Disease |
title_short | Antioxidation Function of EGCG by Activating Nrf2/HO-1 Pathway in Mice with Coronary Heart Disease |
title_sort | antioxidation function of egcg by activating nrf2/ho-1 pathway in mice with coronary heart disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293516/ https://www.ncbi.nlm.nih.gov/pubmed/35919501 http://dx.doi.org/10.1155/2022/8639139 |
work_keys_str_mv | AT huangxiaoyi antioxidationfunctionofegcgbyactivatingnrf2ho1pathwayinmicewithcoronaryheartdisease AT chuyang antioxidationfunctionofegcgbyactivatingnrf2ho1pathwayinmicewithcoronaryheartdisease AT renhua antioxidationfunctionofegcgbyactivatingnrf2ho1pathwayinmicewithcoronaryheartdisease AT pangxiaofen antioxidationfunctionofegcgbyactivatingnrf2ho1pathwayinmicewithcoronaryheartdisease |