Cargando…

MicroID2: A Novel Biotin Ligase Enables Rapid Proximity-Dependent Proteomics

Identifying protein–protein and other proximal interactions is central to dissecting signaling and regulatory processes in cells. BioID is a proximity-dependent biotinylation method that uses an “abortive” biotin ligase to detect proximal interactions in cells in a highly reproducible manner. Recent...

Descripción completa

Detalles Bibliográficos
Autores principales: Johnson, Benjamin S., Chafin, Lexie, Farkas, Daniela, Adair, Jessica, Elhance, Ajit, Farkas, Laszlo, Bednash, Joseph S., Londino, James D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293651/
https://www.ncbi.nlm.nih.gov/pubmed/35688383
http://dx.doi.org/10.1016/j.mcpro.2022.100256