Protocol for culturing and imaging of ectodermal cells from Xenopus

The Xenopus embryo provides an advantageous model system where genes can be readily transplanted as DNA or mRNA or depleted with antisense techniques. Here, we present a protocol to culture and image the cell biological properties of explanted Xenopus cap cells in tissue culture. We illustrate how t...

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Detalles Bibliográficos
Autores principales: Tejeda-Muñoz, Nydia, Monka, Julia, De Robertis, Edward M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293668/
https://www.ncbi.nlm.nih.gov/pubmed/35839770
http://dx.doi.org/10.1016/j.xpro.2022.101455
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author Tejeda-Muñoz, Nydia
Monka, Julia
De Robertis, Edward M.
author_facet Tejeda-Muñoz, Nydia
Monka, Julia
De Robertis, Edward M.
author_sort Tejeda-Muñoz, Nydia
collection PubMed
description The Xenopus embryo provides an advantageous model system where genes can be readily transplanted as DNA or mRNA or depleted with antisense techniques. Here, we present a protocol to culture and image the cell biological properties of explanted Xenopus cap cells in tissue culture. We illustrate how this protocol can be applied to visualize lysosomes, macropinocytosis, focal adhesions, Wnt signaling, and cell migration. For complete details on the use and execution of this protocol, please refer to Tejeda-Muñoz et al. (2022).
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spelling pubmed-92936682022-07-20 Protocol for culturing and imaging of ectodermal cells from Xenopus Tejeda-Muñoz, Nydia Monka, Julia De Robertis, Edward M. STAR Protoc Protocol The Xenopus embryo provides an advantageous model system where genes can be readily transplanted as DNA or mRNA or depleted with antisense techniques. Here, we present a protocol to culture and image the cell biological properties of explanted Xenopus cap cells in tissue culture. We illustrate how this protocol can be applied to visualize lysosomes, macropinocytosis, focal adhesions, Wnt signaling, and cell migration. For complete details on the use and execution of this protocol, please refer to Tejeda-Muñoz et al. (2022). Elsevier 2022-07-14 /pmc/articles/PMC9293668/ /pubmed/35839770 http://dx.doi.org/10.1016/j.xpro.2022.101455 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Tejeda-Muñoz, Nydia
Monka, Julia
De Robertis, Edward M.
Protocol for culturing and imaging of ectodermal cells from Xenopus
title Protocol for culturing and imaging of ectodermal cells from Xenopus
title_full Protocol for culturing and imaging of ectodermal cells from Xenopus
title_fullStr Protocol for culturing and imaging of ectodermal cells from Xenopus
title_full_unstemmed Protocol for culturing and imaging of ectodermal cells from Xenopus
title_short Protocol for culturing and imaging of ectodermal cells from Xenopus
title_sort protocol for culturing and imaging of ectodermal cells from xenopus
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293668/
https://www.ncbi.nlm.nih.gov/pubmed/35839770
http://dx.doi.org/10.1016/j.xpro.2022.101455
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