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Untangling an AGS Outbreak Caused by the Recombinant GII.12[P16] Norovirus With Nanopore Sequencing

For a rapidly spreading virus such as NoV (norovirus), pathogen identification, genotype classification, and transmission tracing are urgent for epidemic control. Here, we applied the Nanopore metatranscriptomic sequencing to determine the causative pathogen of a community AGS (Acute gastroenteritis...

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Autores principales: Xiong, Qianling, Jiang, Huimin, Liu, Zhe, Peng, Jinju, Sun, Jing, Fang, Ling, Li, Caixia, Qiu, Ming, Zhang, Xin, Lu, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294139/
https://www.ncbi.nlm.nih.gov/pubmed/35865812
http://dx.doi.org/10.3389/fcimb.2022.911563
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author Xiong, Qianling
Jiang, Huimin
Liu, Zhe
Peng, Jinju
Sun, Jing
Fang, Ling
Li, Caixia
Qiu, Ming
Zhang, Xin
Lu, Jing
author_facet Xiong, Qianling
Jiang, Huimin
Liu, Zhe
Peng, Jinju
Sun, Jing
Fang, Ling
Li, Caixia
Qiu, Ming
Zhang, Xin
Lu, Jing
author_sort Xiong, Qianling
collection PubMed
description For a rapidly spreading virus such as NoV (norovirus), pathogen identification, genotype classification, and transmission tracing are urgent for epidemic control. Here, we applied the Nanopore metatranscriptomic sequencing to determine the causative pathogen of a community AGS (Acute gastroenteritis) outbreak. The results were also confirmed by RT-PCR. The NGS (Next Generation Sequencing) library was constructed within 8 hours and sequence analyses were carried out in real-time. NoV positive reads were detected in 13 of 17 collected samples, including two water samples from sewage treatment tank and cistern. A nearly complete viral genome and other genome fragments could be generated from metatranscriptomic sequencing of 13 samples. The NoV sequences from water samples and cases are identical suggesting the potential source of the outbreak. The sequencing results also indicated the outbreak was likely caused by an emerging recombinant GII.12[P16] virus, which was only identified in the United States and Canada in 2017–2018. This is the first report of this emerging variant in mainland China, following the large outbreaks caused by the recombinant GII.17[P17] and GII.2[P16] in 2014 and 2016, respectively. Closely monitoring of the prevalence of this recombinant strain is required. Our data also highlighted the importance of real-time sequencing in emerging pathogens’ surveillance.
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spelling pubmed-92941392022-07-20 Untangling an AGS Outbreak Caused by the Recombinant GII.12[P16] Norovirus With Nanopore Sequencing Xiong, Qianling Jiang, Huimin Liu, Zhe Peng, Jinju Sun, Jing Fang, Ling Li, Caixia Qiu, Ming Zhang, Xin Lu, Jing Front Cell Infect Microbiol Cellular and Infection Microbiology For a rapidly spreading virus such as NoV (norovirus), pathogen identification, genotype classification, and transmission tracing are urgent for epidemic control. Here, we applied the Nanopore metatranscriptomic sequencing to determine the causative pathogen of a community AGS (Acute gastroenteritis) outbreak. The results were also confirmed by RT-PCR. The NGS (Next Generation Sequencing) library was constructed within 8 hours and sequence analyses were carried out in real-time. NoV positive reads were detected in 13 of 17 collected samples, including two water samples from sewage treatment tank and cistern. A nearly complete viral genome and other genome fragments could be generated from metatranscriptomic sequencing of 13 samples. The NoV sequences from water samples and cases are identical suggesting the potential source of the outbreak. The sequencing results also indicated the outbreak was likely caused by an emerging recombinant GII.12[P16] virus, which was only identified in the United States and Canada in 2017–2018. This is the first report of this emerging variant in mainland China, following the large outbreaks caused by the recombinant GII.17[P17] and GII.2[P16] in 2014 and 2016, respectively. Closely monitoring of the prevalence of this recombinant strain is required. Our data also highlighted the importance of real-time sequencing in emerging pathogens’ surveillance. Frontiers Media S.A. 2022-07-05 /pmc/articles/PMC9294139/ /pubmed/35865812 http://dx.doi.org/10.3389/fcimb.2022.911563 Text en Copyright © 2022 Xiong, Jiang, Liu, Peng, Sun, Fang, Li, Qiu, Zhang and Lu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Xiong, Qianling
Jiang, Huimin
Liu, Zhe
Peng, Jinju
Sun, Jing
Fang, Ling
Li, Caixia
Qiu, Ming
Zhang, Xin
Lu, Jing
Untangling an AGS Outbreak Caused by the Recombinant GII.12[P16] Norovirus With Nanopore Sequencing
title Untangling an AGS Outbreak Caused by the Recombinant GII.12[P16] Norovirus With Nanopore Sequencing
title_full Untangling an AGS Outbreak Caused by the Recombinant GII.12[P16] Norovirus With Nanopore Sequencing
title_fullStr Untangling an AGS Outbreak Caused by the Recombinant GII.12[P16] Norovirus With Nanopore Sequencing
title_full_unstemmed Untangling an AGS Outbreak Caused by the Recombinant GII.12[P16] Norovirus With Nanopore Sequencing
title_short Untangling an AGS Outbreak Caused by the Recombinant GII.12[P16] Norovirus With Nanopore Sequencing
title_sort untangling an ags outbreak caused by the recombinant gii.12[p16] norovirus with nanopore sequencing
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294139/
https://www.ncbi.nlm.nih.gov/pubmed/35865812
http://dx.doi.org/10.3389/fcimb.2022.911563
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