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Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging
Over 11% of the world’s population experience hearing loss. Although there are promising studies to restore hearing in rodent models, the size, ontogeny, genetics, and frequency range of hearing of most rodents’ cochlea do not match that of humans. The porcine cochlea can bridge this gap as it share...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294204/ https://www.ncbi.nlm.nih.gov/pubmed/35865132 http://dx.doi.org/10.1016/j.isci.2022.104695 |
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author | Moatti, Adele Li, Chen Sivadanam, Sasank Cai, Yuheng Ranta, James Piedrahita, Jorge A. Cheng, Alan G. Ligler, Frances S. Greenbaum, Alon |
author_facet | Moatti, Adele Li, Chen Sivadanam, Sasank Cai, Yuheng Ranta, James Piedrahita, Jorge A. Cheng, Alan G. Ligler, Frances S. Greenbaum, Alon |
author_sort | Moatti, Adele |
collection | PubMed |
description | Over 11% of the world’s population experience hearing loss. Although there are promising studies to restore hearing in rodent models, the size, ontogeny, genetics, and frequency range of hearing of most rodents’ cochlea do not match that of humans. The porcine cochlea can bridge this gap as it shares many anatomical, physiological, and genetic similarities with its human counterpart. Here, we provide a detailed methodology to process and image the porcine cochlea in 3D using tissue clearing and light-sheet microscopy. The resulting 3D images can be employed to compare cochleae across different ages and conditions, investigate the ontogeny of cochlear cytoarchitecture, and produce quantitative expression maps of LGR5, a marker of cochlear progenitors in mice. These data reveal that hair cell organization, inner ear morphology, cellular cartography in the organ of Corti, and spatiotemporal expression of LGR5 are dynamic over developmental stages in a pattern not previously documented. |
format | Online Article Text |
id | pubmed-9294204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-92942042022-07-20 Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging Moatti, Adele Li, Chen Sivadanam, Sasank Cai, Yuheng Ranta, James Piedrahita, Jorge A. Cheng, Alan G. Ligler, Frances S. Greenbaum, Alon iScience Article Over 11% of the world’s population experience hearing loss. Although there are promising studies to restore hearing in rodent models, the size, ontogeny, genetics, and frequency range of hearing of most rodents’ cochlea do not match that of humans. The porcine cochlea can bridge this gap as it shares many anatomical, physiological, and genetic similarities with its human counterpart. Here, we provide a detailed methodology to process and image the porcine cochlea in 3D using tissue clearing and light-sheet microscopy. The resulting 3D images can be employed to compare cochleae across different ages and conditions, investigate the ontogeny of cochlear cytoarchitecture, and produce quantitative expression maps of LGR5, a marker of cochlear progenitors in mice. These data reveal that hair cell organization, inner ear morphology, cellular cartography in the organ of Corti, and spatiotemporal expression of LGR5 are dynamic over developmental stages in a pattern not previously documented. Elsevier 2022-06-30 /pmc/articles/PMC9294204/ /pubmed/35865132 http://dx.doi.org/10.1016/j.isci.2022.104695 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Moatti, Adele Li, Chen Sivadanam, Sasank Cai, Yuheng Ranta, James Piedrahita, Jorge A. Cheng, Alan G. Ligler, Frances S. Greenbaum, Alon Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging |
title | Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging |
title_full | Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging |
title_fullStr | Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging |
title_full_unstemmed | Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging |
title_short | Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging |
title_sort | ontogeny of cellular organization and lgr5 expression in porcine cochlea revealed using tissue clearing and 3d imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294204/ https://www.ncbi.nlm.nih.gov/pubmed/35865132 http://dx.doi.org/10.1016/j.isci.2022.104695 |
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