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Impact of Corticosteroids on the Proportions of Circulating Tfh Cell Subsets in Patients With Systemic Lupus Erythematous
OBJECTIVES: This study aimed to analyze the distribution of T follicular helper (Tfh) cells in lupus patients, and the effects of steroids on circulating Tfh cells. METHODS: Circulating Tfh cell subsets were defined by multicolor flow cytometry as Tfh17, Tfh2 or Tfh1 subpopulations of CXCR5(+)CD45RA...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294243/ https://www.ncbi.nlm.nih.gov/pubmed/35865165 http://dx.doi.org/10.3389/fmed.2022.949334 |
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author | Mao, Minjing Xu, Shuqin Lin, Lin Dong, Danfeng Xue, Minghui He, Siwei Cai, Gang |
author_facet | Mao, Minjing Xu, Shuqin Lin, Lin Dong, Danfeng Xue, Minghui He, Siwei Cai, Gang |
author_sort | Mao, Minjing |
collection | PubMed |
description | OBJECTIVES: This study aimed to analyze the distribution of T follicular helper (Tfh) cells in lupus patients, and the effects of steroids on circulating Tfh cells. METHODS: Circulating Tfh cell subsets were defined by multicolor flow cytometry as Tfh17, Tfh2 or Tfh1 subpopulations of CXCR5(+)CD45RA(–)CD4(+) T cells in the peripheral blood of SLE patients and healthy controls. To test the effects of corticosteroid on Tfh cells, PBMC harvested from both SLE and healthy controls were cocultured with dexamethasone, and then analyzed by Flow cytometry. RESULTS: The proportion of Tfh17 cells in SLE patients was increased significantly compared with healthy controls. Additionally, patients with an active disease had reduced Tfh1 subsets than those with an inactive disease and healthy controls. The frequency of Tfh2 cells was associated with the proportion of circulating plasmablasts and the amount of anti-dsDNA. Dexamethasone reduced the percentage of Tfh2 cells while increased the proportion of Tfh17 subset in gated CXCR5(+)CD45RA(–)CD4(+) T cells. CONCLUSION: Our study investigated the distribution of circulating Tfh subsets in lupus patients. Corticosteroids treatment not only down-regulated the proportion of circulating Tfh cells, but also altered the distribution of Tfh subsets in vivo and in vitro. |
format | Online Article Text |
id | pubmed-9294243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92942432022-07-20 Impact of Corticosteroids on the Proportions of Circulating Tfh Cell Subsets in Patients With Systemic Lupus Erythematous Mao, Minjing Xu, Shuqin Lin, Lin Dong, Danfeng Xue, Minghui He, Siwei Cai, Gang Front Med (Lausanne) Medicine OBJECTIVES: This study aimed to analyze the distribution of T follicular helper (Tfh) cells in lupus patients, and the effects of steroids on circulating Tfh cells. METHODS: Circulating Tfh cell subsets were defined by multicolor flow cytometry as Tfh17, Tfh2 or Tfh1 subpopulations of CXCR5(+)CD45RA(–)CD4(+) T cells in the peripheral blood of SLE patients and healthy controls. To test the effects of corticosteroid on Tfh cells, PBMC harvested from both SLE and healthy controls were cocultured with dexamethasone, and then analyzed by Flow cytometry. RESULTS: The proportion of Tfh17 cells in SLE patients was increased significantly compared with healthy controls. Additionally, patients with an active disease had reduced Tfh1 subsets than those with an inactive disease and healthy controls. The frequency of Tfh2 cells was associated with the proportion of circulating plasmablasts and the amount of anti-dsDNA. Dexamethasone reduced the percentage of Tfh2 cells while increased the proportion of Tfh17 subset in gated CXCR5(+)CD45RA(–)CD4(+) T cells. CONCLUSION: Our study investigated the distribution of circulating Tfh subsets in lupus patients. Corticosteroids treatment not only down-regulated the proportion of circulating Tfh cells, but also altered the distribution of Tfh subsets in vivo and in vitro. Frontiers Media S.A. 2022-07-05 /pmc/articles/PMC9294243/ /pubmed/35865165 http://dx.doi.org/10.3389/fmed.2022.949334 Text en Copyright © 2022 Mao, Xu, Lin, Dong, Xue, He and Cai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Mao, Minjing Xu, Shuqin Lin, Lin Dong, Danfeng Xue, Minghui He, Siwei Cai, Gang Impact of Corticosteroids on the Proportions of Circulating Tfh Cell Subsets in Patients With Systemic Lupus Erythematous |
title | Impact of Corticosteroids on the Proportions of Circulating Tfh Cell Subsets in Patients With Systemic Lupus Erythematous |
title_full | Impact of Corticosteroids on the Proportions of Circulating Tfh Cell Subsets in Patients With Systemic Lupus Erythematous |
title_fullStr | Impact of Corticosteroids on the Proportions of Circulating Tfh Cell Subsets in Patients With Systemic Lupus Erythematous |
title_full_unstemmed | Impact of Corticosteroids on the Proportions of Circulating Tfh Cell Subsets in Patients With Systemic Lupus Erythematous |
title_short | Impact of Corticosteroids on the Proportions of Circulating Tfh Cell Subsets in Patients With Systemic Lupus Erythematous |
title_sort | impact of corticosteroids on the proportions of circulating tfh cell subsets in patients with systemic lupus erythematous |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294243/ https://www.ncbi.nlm.nih.gov/pubmed/35865165 http://dx.doi.org/10.3389/fmed.2022.949334 |
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