Generating patient-derived ascites-dependent xenograft mouse models of peritoneal carcinomatosis

Clinically relevant animal models are crucial for effective development of therapeutics for peritoneal carcinomatosis (PC). This protocol describes the generation of patient-derived ascites-dependent xenograft (PDADX) models from the cellular component of ascites. The use of routine intraperitoneal...

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Detalles Bibliográficos
Autores principales: Liu, Ying, Ng, Wai Har, Zhu, Hong-Yuan, Tan, Qiu Xuan, Hendrikson, Josephine, Tan, Joey Wee-Shan, Ng, Gillian, Lim, Weng Khong, Ong, Chin-Ann Johnny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294250/
https://www.ncbi.nlm.nih.gov/pubmed/35842864
http://dx.doi.org/10.1016/j.xpro.2022.101548
Descripción
Sumario:Clinically relevant animal models are crucial for effective development of therapeutics for peritoneal carcinomatosis (PC). This protocol describes the generation of patient-derived ascites-dependent xenograft (PDADX) models from the cellular component of ascites. The use of routine intraperitoneal injection of the fluid component of ascites is analogous to the biological events occurring intra-abdominally in patients with PC. By serving as a proxy, PDADX models represent a valuable tool for preclinical testing of new therapeutics for PC. For complete details on the use and execution of this protocol, please refer to Hendrikson et al. (2022).

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