Long-term SCN calcium signal recording in freely moving mice

The suprachiasmatic nucleus (SCN) is the master circadian pacemaker of the mammalian biological clock. Here, we provide a detailed protocol for long-term recording of calcium signals in SCN neurons of freely moving mice through a multichannel optical fiber recording system. This system can simultane...

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Detalles Bibliográficos
Autores principales: Zhai, Qiaocheng, Zeng, Yizhun, Li, Zhihao, Xu, Youjia, Xu, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294265/
https://www.ncbi.nlm.nih.gov/pubmed/35842865
http://dx.doi.org/10.1016/j.xpro.2022.101547
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author Zhai, Qiaocheng
Zeng, Yizhun
Li, Zhihao
Xu, Youjia
Xu, Ying
author_facet Zhai, Qiaocheng
Zeng, Yizhun
Li, Zhihao
Xu, Youjia
Xu, Ying
author_sort Zhai, Qiaocheng
collection PubMed
description The suprachiasmatic nucleus (SCN) is the master circadian pacemaker of the mammalian biological clock. Here, we provide a detailed protocol for long-term recording of calcium signals in SCN neurons of freely moving mice through a multichannel optical fiber recording system. This system can simultaneously collect calcium signals from up to seven animals. The calcium signals can be visualized by the appropriate software and code. This protocol can be used to explore the long-term response of SCN to external environmental stimulation. For complete details on the use and execution of this protocol, please refer to Zhai et al. (2022).
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spelling pubmed-92942652022-07-20 Long-term SCN calcium signal recording in freely moving mice Zhai, Qiaocheng Zeng, Yizhun Li, Zhihao Xu, Youjia Xu, Ying STAR Protoc Protocol The suprachiasmatic nucleus (SCN) is the master circadian pacemaker of the mammalian biological clock. Here, we provide a detailed protocol for long-term recording of calcium signals in SCN neurons of freely moving mice through a multichannel optical fiber recording system. This system can simultaneously collect calcium signals from up to seven animals. The calcium signals can be visualized by the appropriate software and code. This protocol can be used to explore the long-term response of SCN to external environmental stimulation. For complete details on the use and execution of this protocol, please refer to Zhai et al. (2022). Elsevier 2022-07-16 /pmc/articles/PMC9294265/ /pubmed/35842865 http://dx.doi.org/10.1016/j.xpro.2022.101547 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhai, Qiaocheng
Zeng, Yizhun
Li, Zhihao
Xu, Youjia
Xu, Ying
Long-term SCN calcium signal recording in freely moving mice
title Long-term SCN calcium signal recording in freely moving mice
title_full Long-term SCN calcium signal recording in freely moving mice
title_fullStr Long-term SCN calcium signal recording in freely moving mice
title_full_unstemmed Long-term SCN calcium signal recording in freely moving mice
title_short Long-term SCN calcium signal recording in freely moving mice
title_sort long-term scn calcium signal recording in freely moving mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294265/
https://www.ncbi.nlm.nih.gov/pubmed/35842865
http://dx.doi.org/10.1016/j.xpro.2022.101547
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AT xuyoujia longtermscncalciumsignalrecordinginfreelymovingmice
AT xuying longtermscncalciumsignalrecordinginfreelymovingmice

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