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Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary

In this study, the stereo-pathological effect of metformin and N-acetyl cysteine is evaluated on the uterus and ovary of polycystic ovary syndrome (PCOS) mice. 96 mature females (8-weekold, weight of 20–30 gr) BALB/c mice were classified into 6 groups including the control group (n= 16), letrozole-i...

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Autores principales: Rafiee, Bahare, Karbalay-doust, Saied, Tabei, Seyed Mohammad Bagher, Azarpira, Negar, Alaee, Sanaz, Lohrasbi, Parvin, Bahmanpour, Soghra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295164/
https://www.ncbi.nlm.nih.gov/pubmed/35535444
http://dx.doi.org/10.4081/ejtm.2022.10409
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author Rafiee, Bahare
Karbalay-doust, Saied
Tabei, Seyed Mohammad Bagher
Azarpira, Negar
Alaee, Sanaz
Lohrasbi, Parvin
Bahmanpour, Soghra
author_facet Rafiee, Bahare
Karbalay-doust, Saied
Tabei, Seyed Mohammad Bagher
Azarpira, Negar
Alaee, Sanaz
Lohrasbi, Parvin
Bahmanpour, Soghra
author_sort Rafiee, Bahare
collection PubMed
description In this study, the stereo-pathological effect of metformin and N-acetyl cysteine is evaluated on the uterus and ovary of polycystic ovary syndrome (PCOS) mice. 96 mature females (8-weekold, weight of 20–30 gr) BALB/c mice were classified into 6 groups including the control group (n= 16), letrozole-induced PCOS group (n=16), PCOS + metformin (n=16), PCOS+NAC (n=16) and a separate control group for NAC (n=16). Another PCOS group was maintained for a month to make sure that features remain till the end of the study. Testosterone level, vaginal cytology and stereological evaluations were assessed. Vaginal cytology in letrozole-receiving mice showed a diestrus phase continuity. Testosterone level, body weight, uterine weight, endometrial volume, myometrial volume, gland volume, stromal volume, epithelial volume, vessel volume, daughter and conglomerate glands, endometrial thickness, and myometrial thickness exhibited an increasing trend in the uterus of PCOS mice. While normal gland and vessel length decreased in the PCOS group. Ovarian volume, corticomedullary volume, primary follicles, secondary follicles, and ovarian cysts were increased in PCOS ovaries. While corpus luteum, primordial, graafian, and atretic follicles showed a decline in the PCOS group. NAC and metformin, however, managed to restore the condition to normal. Given the prevalence of PCOS and its impact on fertility, the use of noninvasive methods is of crucial significance. NAC can control and treat pathological parameters and help as a harmless drug in the treatment of women with PCOS.
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spelling pubmed-92951642022-07-20 Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary Rafiee, Bahare Karbalay-doust, Saied Tabei, Seyed Mohammad Bagher Azarpira, Negar Alaee, Sanaz Lohrasbi, Parvin Bahmanpour, Soghra Eur J Transl Myol Article In this study, the stereo-pathological effect of metformin and N-acetyl cysteine is evaluated on the uterus and ovary of polycystic ovary syndrome (PCOS) mice. 96 mature females (8-weekold, weight of 20–30 gr) BALB/c mice were classified into 6 groups including the control group (n= 16), letrozole-induced PCOS group (n=16), PCOS + metformin (n=16), PCOS+NAC (n=16) and a separate control group for NAC (n=16). Another PCOS group was maintained for a month to make sure that features remain till the end of the study. Testosterone level, vaginal cytology and stereological evaluations were assessed. Vaginal cytology in letrozole-receiving mice showed a diestrus phase continuity. Testosterone level, body weight, uterine weight, endometrial volume, myometrial volume, gland volume, stromal volume, epithelial volume, vessel volume, daughter and conglomerate glands, endometrial thickness, and myometrial thickness exhibited an increasing trend in the uterus of PCOS mice. While normal gland and vessel length decreased in the PCOS group. Ovarian volume, corticomedullary volume, primary follicles, secondary follicles, and ovarian cysts were increased in PCOS ovaries. While corpus luteum, primordial, graafian, and atretic follicles showed a decline in the PCOS group. NAC and metformin, however, managed to restore the condition to normal. Given the prevalence of PCOS and its impact on fertility, the use of noninvasive methods is of crucial significance. NAC can control and treat pathological parameters and help as a harmless drug in the treatment of women with PCOS. PAGEPress Publications, Pavia, Italy 2022-05-09 /pmc/articles/PMC9295164/ /pubmed/35535444 http://dx.doi.org/10.4081/ejtm.2022.10409 Text en https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Rafiee, Bahare
Karbalay-doust, Saied
Tabei, Seyed Mohammad Bagher
Azarpira, Negar
Alaee, Sanaz
Lohrasbi, Parvin
Bahmanpour, Soghra
Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary
title Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary
title_full Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary
title_fullStr Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary
title_full_unstemmed Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary
title_short Effects of N-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary
title_sort effects of n-acetylcysteine and metformin treatment on the stereopathological characteristics of uterus and ovary
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295164/
https://www.ncbi.nlm.nih.gov/pubmed/35535444
http://dx.doi.org/10.4081/ejtm.2022.10409
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