Cargando…

MdNup54 Interactions With MdHSP70 Involved in Flowering in Apple

Flowering-related problems in “Fuji” apple have severely restricted the development of China’s apple industry. Nuclear pore complexes (NPCs) control nucleoplasmic transport and play an important role in the regulation of plant growth and development. However, the effects of NPCs on apple flowering h...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Chenguang, Zhang, XIaoshuang, Cheng, Bo, Wu, Junkai, Zhang, Libin, Xiao, Xiao, Zhang, Dong, Zhao, Caiping, An, Na, Han, Mingyu, Xing, Libo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296068/
https://www.ncbi.nlm.nih.gov/pubmed/35865288
http://dx.doi.org/10.3389/fpls.2022.903808
_version_ 1784750185330507776
author Zhang, Chenguang
Zhang, XIaoshuang
Cheng, Bo
Wu, Junkai
Zhang, Libin
Xiao, Xiao
Zhang, Dong
Zhao, Caiping
An, Na
Han, Mingyu
Xing, Libo
author_facet Zhang, Chenguang
Zhang, XIaoshuang
Cheng, Bo
Wu, Junkai
Zhang, Libin
Xiao, Xiao
Zhang, Dong
Zhao, Caiping
An, Na
Han, Mingyu
Xing, Libo
author_sort Zhang, Chenguang
collection PubMed
description Flowering-related problems in “Fuji” apple have severely restricted the development of China’s apple industry. Nuclear pore complexes (NPCs) control nucleoplasmic transport and play an important role in the regulation of plant growth and development. However, the effects of NPCs on apple flowering have not been reported. Here, we analysed the expression and function of MdNup54, a component of apple NPC. MdNup54 expression was the highest in flower buds and maintained during 30–70 days after flowering. MdNup54-overexpressing (OE) Arabidopsis lines displayed significantly earlier flowering than that of the wild type. We further confirmed that MdNup54 interacts with MdHSP70, MdMYB11, and MdKNAT4/6. Consistent with these observations, flowering time of MdHSP70-OE Arabidopsis lines was also significantly earlier. Therefore, our findings suggest a possible interaction of MdNup54 with MdHSP70 to mediate its nuclear and cytoplasmic transport and to regulate apple flowering. The results enhance the understanding of the flowering mechanism in apple and propose a novel strategy to study nucleoporins.
format Online
Article
Text
id pubmed-9296068
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92960682022-07-20 MdNup54 Interactions With MdHSP70 Involved in Flowering in Apple Zhang, Chenguang Zhang, XIaoshuang Cheng, Bo Wu, Junkai Zhang, Libin Xiao, Xiao Zhang, Dong Zhao, Caiping An, Na Han, Mingyu Xing, Libo Front Plant Sci Plant Science Flowering-related problems in “Fuji” apple have severely restricted the development of China’s apple industry. Nuclear pore complexes (NPCs) control nucleoplasmic transport and play an important role in the regulation of plant growth and development. However, the effects of NPCs on apple flowering have not been reported. Here, we analysed the expression and function of MdNup54, a component of apple NPC. MdNup54 expression was the highest in flower buds and maintained during 30–70 days after flowering. MdNup54-overexpressing (OE) Arabidopsis lines displayed significantly earlier flowering than that of the wild type. We further confirmed that MdNup54 interacts with MdHSP70, MdMYB11, and MdKNAT4/6. Consistent with these observations, flowering time of MdHSP70-OE Arabidopsis lines was also significantly earlier. Therefore, our findings suggest a possible interaction of MdNup54 with MdHSP70 to mediate its nuclear and cytoplasmic transport and to regulate apple flowering. The results enhance the understanding of the flowering mechanism in apple and propose a novel strategy to study nucleoporins. Frontiers Media S.A. 2022-07-05 /pmc/articles/PMC9296068/ /pubmed/35865288 http://dx.doi.org/10.3389/fpls.2022.903808 Text en Copyright © 2022 Zhang, Zhang, Cheng, Wu, Zhang, Xiao, Zhang, Zhao, An, Han and Xing. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Chenguang
Zhang, XIaoshuang
Cheng, Bo
Wu, Junkai
Zhang, Libin
Xiao, Xiao
Zhang, Dong
Zhao, Caiping
An, Na
Han, Mingyu
Xing, Libo
MdNup54 Interactions With MdHSP70 Involved in Flowering in Apple
title MdNup54 Interactions With MdHSP70 Involved in Flowering in Apple
title_full MdNup54 Interactions With MdHSP70 Involved in Flowering in Apple
title_fullStr MdNup54 Interactions With MdHSP70 Involved in Flowering in Apple
title_full_unstemmed MdNup54 Interactions With MdHSP70 Involved in Flowering in Apple
title_short MdNup54 Interactions With MdHSP70 Involved in Flowering in Apple
title_sort mdnup54 interactions with mdhsp70 involved in flowering in apple
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296068/
https://www.ncbi.nlm.nih.gov/pubmed/35865288
http://dx.doi.org/10.3389/fpls.2022.903808
work_keys_str_mv AT zhangchenguang mdnup54interactionswithmdhsp70involvedinfloweringinapple
AT zhangxiaoshuang mdnup54interactionswithmdhsp70involvedinfloweringinapple
AT chengbo mdnup54interactionswithmdhsp70involvedinfloweringinapple
AT wujunkai mdnup54interactionswithmdhsp70involvedinfloweringinapple
AT zhanglibin mdnup54interactionswithmdhsp70involvedinfloweringinapple
AT xiaoxiao mdnup54interactionswithmdhsp70involvedinfloweringinapple
AT zhangdong mdnup54interactionswithmdhsp70involvedinfloweringinapple
AT zhaocaiping mdnup54interactionswithmdhsp70involvedinfloweringinapple
AT anna mdnup54interactionswithmdhsp70involvedinfloweringinapple
AT hanmingyu mdnup54interactionswithmdhsp70involvedinfloweringinapple
AT xinglibo mdnup54interactionswithmdhsp70involvedinfloweringinapple