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Impact of Geographical Location on the Gut Microbiota Profile in Egyptian Children with Type 1 Diabetes Mellitus: A Pilot Study

PURPOSE: To investigate the compositional and functional characteristics of T1DM-associated gut microbiota in two Egyptian cities and to study the geographical locality effects. PATIENTS AND METHODS: This case-control study included 32 children with controlled T1DM and 16 controls, selected from two...

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Autores principales: Elsherbiny, Nahla M, Ramadan, Mohammed, Abu Faddan, Nagla H, Hassan, Elham Ahmed, Ali, Mohamed E, Abd El-Rehim, Abeer Sharaf El-Din, Abbas, Wael A, Abozaid, Mohamed A A, Hassanin, Ebtisam, Mohamed, Ghada A, Hetta, Helal F, Salah, Mohammed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296103/
https://www.ncbi.nlm.nih.gov/pubmed/35864993
http://dx.doi.org/10.2147/IJGM.S361169
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author Elsherbiny, Nahla M
Ramadan, Mohammed
Abu Faddan, Nagla H
Hassan, Elham Ahmed
Ali, Mohamed E
Abd El-Rehim, Abeer Sharaf El-Din
Abbas, Wael A
Abozaid, Mohamed A A
Hassanin, Ebtisam
Mohamed, Ghada A
Hetta, Helal F
Salah, Mohammed
author_facet Elsherbiny, Nahla M
Ramadan, Mohammed
Abu Faddan, Nagla H
Hassan, Elham Ahmed
Ali, Mohamed E
Abd El-Rehim, Abeer Sharaf El-Din
Abbas, Wael A
Abozaid, Mohamed A A
Hassanin, Ebtisam
Mohamed, Ghada A
Hetta, Helal F
Salah, Mohammed
author_sort Elsherbiny, Nahla M
collection PubMed
description PURPOSE: To investigate the compositional and functional characteristics of T1DM-associated gut microbiota in two Egyptian cities and to study the geographical locality effects. PATIENTS AND METHODS: This case-control study included 32 children with controlled T1DM and 16 controls, selected from two different regions of Egypt. The gut microbiota of both diabetic and control children was analyzed through 16S rRNA gene sequencing; this was done using the Illumina MiSeq platform. RESULTS: Consistent findings among the diabetic children included significantly lower alpha diversity than the control children, as well as a lower mean Firmicutes/Bacteroidetes (F/B) ratio, and reduced proportions of Firmicutes and the genera Prevotella and Ruminococcus. In the diabetic children, there were also significantly enriched representations of Actinobacteria, Bacteroidetes, and Proteobacteria and the genera Lactobacilli, Bacteroides, and Faecalibacterium. When comparing the two diabetic groups, the Ismailia group (IsDM) was found to have a significantly higher F/B ratio and diversity indices, with resultant differences at the functional level. CONCLUSION: There are a number of consistent changes in the microbiota profile characterizing the diabetic groups irrespective of the geographical location including significantly lower alpha diversity, mean Firmicutes/ Bacteroidetes (F/B) ratio, and reduced proportions of Firmicutes and genera Prevotella and Ruminococcus. There are also significantly enriched representations of Actinobacteria, Bacteroidetes, and Proteobacteria and genera Lactobacilli, Bacteroides, and Faecalibacterium pointing to the greater driving power of the disease.
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spelling pubmed-92961032022-07-20 Impact of Geographical Location on the Gut Microbiota Profile in Egyptian Children with Type 1 Diabetes Mellitus: A Pilot Study Elsherbiny, Nahla M Ramadan, Mohammed Abu Faddan, Nagla H Hassan, Elham Ahmed Ali, Mohamed E Abd El-Rehim, Abeer Sharaf El-Din Abbas, Wael A Abozaid, Mohamed A A Hassanin, Ebtisam Mohamed, Ghada A Hetta, Helal F Salah, Mohammed Int J Gen Med Original Research PURPOSE: To investigate the compositional and functional characteristics of T1DM-associated gut microbiota in two Egyptian cities and to study the geographical locality effects. PATIENTS AND METHODS: This case-control study included 32 children with controlled T1DM and 16 controls, selected from two different regions of Egypt. The gut microbiota of both diabetic and control children was analyzed through 16S rRNA gene sequencing; this was done using the Illumina MiSeq platform. RESULTS: Consistent findings among the diabetic children included significantly lower alpha diversity than the control children, as well as a lower mean Firmicutes/Bacteroidetes (F/B) ratio, and reduced proportions of Firmicutes and the genera Prevotella and Ruminococcus. In the diabetic children, there were also significantly enriched representations of Actinobacteria, Bacteroidetes, and Proteobacteria and the genera Lactobacilli, Bacteroides, and Faecalibacterium. When comparing the two diabetic groups, the Ismailia group (IsDM) was found to have a significantly higher F/B ratio and diversity indices, with resultant differences at the functional level. CONCLUSION: There are a number of consistent changes in the microbiota profile characterizing the diabetic groups irrespective of the geographical location including significantly lower alpha diversity, mean Firmicutes/ Bacteroidetes (F/B) ratio, and reduced proportions of Firmicutes and genera Prevotella and Ruminococcus. There are also significantly enriched representations of Actinobacteria, Bacteroidetes, and Proteobacteria and genera Lactobacilli, Bacteroides, and Faecalibacterium pointing to the greater driving power of the disease. Dove 2022-07-15 /pmc/articles/PMC9296103/ /pubmed/35864993 http://dx.doi.org/10.2147/IJGM.S361169 Text en © 2022 Elsherbiny et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Elsherbiny, Nahla M
Ramadan, Mohammed
Abu Faddan, Nagla H
Hassan, Elham Ahmed
Ali, Mohamed E
Abd El-Rehim, Abeer Sharaf El-Din
Abbas, Wael A
Abozaid, Mohamed A A
Hassanin, Ebtisam
Mohamed, Ghada A
Hetta, Helal F
Salah, Mohammed
Impact of Geographical Location on the Gut Microbiota Profile in Egyptian Children with Type 1 Diabetes Mellitus: A Pilot Study
title Impact of Geographical Location on the Gut Microbiota Profile in Egyptian Children with Type 1 Diabetes Mellitus: A Pilot Study
title_full Impact of Geographical Location on the Gut Microbiota Profile in Egyptian Children with Type 1 Diabetes Mellitus: A Pilot Study
title_fullStr Impact of Geographical Location on the Gut Microbiota Profile in Egyptian Children with Type 1 Diabetes Mellitus: A Pilot Study
title_full_unstemmed Impact of Geographical Location on the Gut Microbiota Profile in Egyptian Children with Type 1 Diabetes Mellitus: A Pilot Study
title_short Impact of Geographical Location on the Gut Microbiota Profile in Egyptian Children with Type 1 Diabetes Mellitus: A Pilot Study
title_sort impact of geographical location on the gut microbiota profile in egyptian children with type 1 diabetes mellitus: a pilot study
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296103/
https://www.ncbi.nlm.nih.gov/pubmed/35864993
http://dx.doi.org/10.2147/IJGM.S361169
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