Cargando…
The Effects of Poria cocos on Rho Signaling-Induced Regulation of Mobility and F-Actin Aggregation in MK-801-Treated B35 and C6 Cells
METHODS: B35 neuronal cells and C6 glial cells were incubated with MK-801 for 7 days followed by MK-801, MK801 in combination with water extracts of P. cocos (PRP for P. cocos cum Radix Pini or WP for White Poria) treatment for an additional 7 days. Analysis of cell mobility, F-actin aggregation, an...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296330/ https://www.ncbi.nlm.nih.gov/pubmed/35864844 http://dx.doi.org/10.1155/2022/8225499 |
_version_ | 1784750250000384000 |
---|---|
author | Chen, Yi-Chyan Lee, Chang-Ti Tsai, Fu-Ming Chen, Mao-Liang |
author_facet | Chen, Yi-Chyan Lee, Chang-Ti Tsai, Fu-Ming Chen, Mao-Liang |
author_sort | Chen, Yi-Chyan |
collection | PubMed |
description | METHODS: B35 neuronal cells and C6 glial cells were incubated with MK-801 for 7 days followed by MK-801, MK801 in combination with water extracts of P. cocos (PRP for P. cocos cum Radix Pini or WP for White Poria) treatment for an additional 7 days. Analysis of cell mobility, F-actin aggregation, and Rho signaling modulation was performed to clarify the roles of PRP or WP in MK-801-treated B35 and C6 cells. RESULTS: MK-801 decreases B35 cell mobility, whereas the inhibited cell migration ability and F-actin aggregation in MK-801-treated B35 or C6 cells could be reversed by PRP or WP. The CDC42 expression in B35 or C6 cells would be reduced by MK-801 and restored by treating with PRP or WP. The RhoA expression was increased by MK-801 in both B35 and C6 cells but was differentially regulated by PRP or WP. In B35 cells, downregulation of PFN1, N-WASP, PAK1, and ARP2/3 induced by MK-801 can be reversely modulated by PRP or WP. PRP or WP reduced the increase in the p-MLC2 expression in B35 cells treated with MK-801. The reduction in ROCK1, PFN1, p-MLC2, and ARP2/3 expression in C6 cells induced by MK-801 was restored by PRP or WP. Reduced N-WASP and PAK1 expression was differentially regulated by PRP or WP in MK-801-treated C6 cells. |
format | Online Article Text |
id | pubmed-9296330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-92963302022-07-20 The Effects of Poria cocos on Rho Signaling-Induced Regulation of Mobility and F-Actin Aggregation in MK-801-Treated B35 and C6 Cells Chen, Yi-Chyan Lee, Chang-Ti Tsai, Fu-Ming Chen, Mao-Liang Behav Neurol Research Article METHODS: B35 neuronal cells and C6 glial cells were incubated with MK-801 for 7 days followed by MK-801, MK801 in combination with water extracts of P. cocos (PRP for P. cocos cum Radix Pini or WP for White Poria) treatment for an additional 7 days. Analysis of cell mobility, F-actin aggregation, and Rho signaling modulation was performed to clarify the roles of PRP or WP in MK-801-treated B35 and C6 cells. RESULTS: MK-801 decreases B35 cell mobility, whereas the inhibited cell migration ability and F-actin aggregation in MK-801-treated B35 or C6 cells could be reversed by PRP or WP. The CDC42 expression in B35 or C6 cells would be reduced by MK-801 and restored by treating with PRP or WP. The RhoA expression was increased by MK-801 in both B35 and C6 cells but was differentially regulated by PRP or WP. In B35 cells, downregulation of PFN1, N-WASP, PAK1, and ARP2/3 induced by MK-801 can be reversely modulated by PRP or WP. PRP or WP reduced the increase in the p-MLC2 expression in B35 cells treated with MK-801. The reduction in ROCK1, PFN1, p-MLC2, and ARP2/3 expression in C6 cells induced by MK-801 was restored by PRP or WP. Reduced N-WASP and PAK1 expression was differentially regulated by PRP or WP in MK-801-treated C6 cells. Hindawi 2022-07-12 /pmc/articles/PMC9296330/ /pubmed/35864844 http://dx.doi.org/10.1155/2022/8225499 Text en Copyright © 2022 Yi-Chyan Chen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Yi-Chyan Lee, Chang-Ti Tsai, Fu-Ming Chen, Mao-Liang The Effects of Poria cocos on Rho Signaling-Induced Regulation of Mobility and F-Actin Aggregation in MK-801-Treated B35 and C6 Cells |
title | The Effects of Poria cocos on Rho Signaling-Induced Regulation of Mobility and F-Actin Aggregation in MK-801-Treated B35 and C6 Cells |
title_full | The Effects of Poria cocos on Rho Signaling-Induced Regulation of Mobility and F-Actin Aggregation in MK-801-Treated B35 and C6 Cells |
title_fullStr | The Effects of Poria cocos on Rho Signaling-Induced Regulation of Mobility and F-Actin Aggregation in MK-801-Treated B35 and C6 Cells |
title_full_unstemmed | The Effects of Poria cocos on Rho Signaling-Induced Regulation of Mobility and F-Actin Aggregation in MK-801-Treated B35 and C6 Cells |
title_short | The Effects of Poria cocos on Rho Signaling-Induced Regulation of Mobility and F-Actin Aggregation in MK-801-Treated B35 and C6 Cells |
title_sort | effects of poria cocos on rho signaling-induced regulation of mobility and f-actin aggregation in mk-801-treated b35 and c6 cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296330/ https://www.ncbi.nlm.nih.gov/pubmed/35864844 http://dx.doi.org/10.1155/2022/8225499 |
work_keys_str_mv | AT chenyichyan theeffectsofporiacocosonrhosignalinginducedregulationofmobilityandfactinaggregationinmk801treatedb35andc6cells AT leechangti theeffectsofporiacocosonrhosignalinginducedregulationofmobilityandfactinaggregationinmk801treatedb35andc6cells AT tsaifuming theeffectsofporiacocosonrhosignalinginducedregulationofmobilityandfactinaggregationinmk801treatedb35andc6cells AT chenmaoliang theeffectsofporiacocosonrhosignalinginducedregulationofmobilityandfactinaggregationinmk801treatedb35andc6cells AT chenyichyan effectsofporiacocosonrhosignalinginducedregulationofmobilityandfactinaggregationinmk801treatedb35andc6cells AT leechangti effectsofporiacocosonrhosignalinginducedregulationofmobilityandfactinaggregationinmk801treatedb35andc6cells AT tsaifuming effectsofporiacocosonrhosignalinginducedregulationofmobilityandfactinaggregationinmk801treatedb35andc6cells AT chenmaoliang effectsofporiacocosonrhosignalinginducedregulationofmobilityandfactinaggregationinmk801treatedb35andc6cells |