Detection of cardiac apoptosis by [(18)F]ML-10 in a mouse model of permanent LAD ligation

PURPOSE: The loss of viable cardiac cells and cell death by myocardial infarction (MI) is still a significant obstacle in preventing deteriorating heart failure. Imaging of apoptosis, a defined cascade to cell death, could identify areas at risk. PROCEDURES: Using 2-(5-[(18)F]fluoropentyl)-2-methyl-...

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Autores principales: Fischer, Maximilian, Olivier, Jessica, Lindner, Simon, Zacherl, Mathias J., Massberg, Steffen, Bartenstein, Peter, Ziegler, Sibylle, Brendel, Matthias, Lehner, Sebastian, Boening, Guido, Todica, Andrei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
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Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296384/
https://www.ncbi.nlm.nih.gov/pubmed/35352214
http://dx.doi.org/10.1007/s11307-022-01718-0
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author Fischer, Maximilian
Olivier, Jessica
Lindner, Simon
Zacherl, Mathias J.
Massberg, Steffen
Bartenstein, Peter
Ziegler, Sibylle
Brendel, Matthias
Lehner, Sebastian
Boening, Guido
Todica, Andrei
author_facet Fischer, Maximilian
Olivier, Jessica
Lindner, Simon
Zacherl, Mathias J.
Massberg, Steffen
Bartenstein, Peter
Ziegler, Sibylle
Brendel, Matthias
Lehner, Sebastian
Boening, Guido
Todica, Andrei
author_sort Fischer, Maximilian
collection PubMed
description PURPOSE: The loss of viable cardiac cells and cell death by myocardial infarction (MI) is still a significant obstacle in preventing deteriorating heart failure. Imaging of apoptosis, a defined cascade to cell death, could identify areas at risk. PROCEDURES: Using 2-(5-[(18)F]fluoropentyl)-2-methyl-malonic acid ([(18)F]ML-10) in autoradiography and positron emission tomography (PET) visualized apoptosis in murine hearts after permanent ligation of the left anterior descending artery (LAD) inducing myocardial infarction (MI). 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) PET imaging localized the infarct area after MI. Histology by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining validated apoptosis in the heart. RESULTS: Accumulation of [(18)F]ML-10 was evident in the infarct area after permanent ligation of the LAD in autoradiography and PET imaging. Detection of apoptosis by [(18)F]ML-10 is in line with the defect visualized by [(18)F]FDG and the histological approach. CONCLUSION: [(18)F]ML-10 could be a suitable tracer for apoptosis imaging in a mouse model of permanent LAD ligation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11307-022-01718-0.
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spelling pubmed-92963842022-07-21 Detection of cardiac apoptosis by [(18)F]ML-10 in a mouse model of permanent LAD ligation Fischer, Maximilian Olivier, Jessica Lindner, Simon Zacherl, Mathias J. Massberg, Steffen Bartenstein, Peter Ziegler, Sibylle Brendel, Matthias Lehner, Sebastian Boening, Guido Todica, Andrei Mol Imaging Biol Research Article PURPOSE: The loss of viable cardiac cells and cell death by myocardial infarction (MI) is still a significant obstacle in preventing deteriorating heart failure. Imaging of apoptosis, a defined cascade to cell death, could identify areas at risk. PROCEDURES: Using 2-(5-[(18)F]fluoropentyl)-2-methyl-malonic acid ([(18)F]ML-10) in autoradiography and positron emission tomography (PET) visualized apoptosis in murine hearts after permanent ligation of the left anterior descending artery (LAD) inducing myocardial infarction (MI). 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) PET imaging localized the infarct area after MI. Histology by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining validated apoptosis in the heart. RESULTS: Accumulation of [(18)F]ML-10 was evident in the infarct area after permanent ligation of the LAD in autoradiography and PET imaging. Detection of apoptosis by [(18)F]ML-10 is in line with the defect visualized by [(18)F]FDG and the histological approach. CONCLUSION: [(18)F]ML-10 could be a suitable tracer for apoptosis imaging in a mouse model of permanent LAD ligation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11307-022-01718-0. Springer International Publishing 2022-03-29 2022 /pmc/articles/PMC9296384/ /pubmed/35352214 http://dx.doi.org/10.1007/s11307-022-01718-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Fischer, Maximilian
Olivier, Jessica
Lindner, Simon
Zacherl, Mathias J.
Massberg, Steffen
Bartenstein, Peter
Ziegler, Sibylle
Brendel, Matthias
Lehner, Sebastian
Boening, Guido
Todica, Andrei
Detection of cardiac apoptosis by [(18)F]ML-10 in a mouse model of permanent LAD ligation
title Detection of cardiac apoptosis by [(18)F]ML-10 in a mouse model of permanent LAD ligation
title_full Detection of cardiac apoptosis by [(18)F]ML-10 in a mouse model of permanent LAD ligation
title_fullStr Detection of cardiac apoptosis by [(18)F]ML-10 in a mouse model of permanent LAD ligation
title_full_unstemmed Detection of cardiac apoptosis by [(18)F]ML-10 in a mouse model of permanent LAD ligation
title_short Detection of cardiac apoptosis by [(18)F]ML-10 in a mouse model of permanent LAD ligation
title_sort detection of cardiac apoptosis by [(18)f]ml-10 in a mouse model of permanent lad ligation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296384/
https://www.ncbi.nlm.nih.gov/pubmed/35352214
http://dx.doi.org/10.1007/s11307-022-01718-0
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