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Rapid high-resolution volumetric imaging via laser ablation delayering and confocal imaging
Acquiring detailed 3D images of samples is needed for conducting thorough investigations in a wide range of applications. Doing so using nondestructive methods such as X-ray computed tomography (X-ray CT) has resolution limitations. Destructive methods, which work based on consecutive delayering and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296531/ https://www.ncbi.nlm.nih.gov/pubmed/35853990 http://dx.doi.org/10.1038/s41598-022-16519-2 |
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author | Phoulady, Adrian May, Nicholas Choi, Hongbin Suleiman, Yara Shahbazmohamadi, Sina Tavousi, Pouya |
author_facet | Phoulady, Adrian May, Nicholas Choi, Hongbin Suleiman, Yara Shahbazmohamadi, Sina Tavousi, Pouya |
author_sort | Phoulady, Adrian |
collection | PubMed |
description | Acquiring detailed 3D images of samples is needed for conducting thorough investigations in a wide range of applications. Doing so using nondestructive methods such as X-ray computed tomography (X-ray CT) has resolution limitations. Destructive methods, which work based on consecutive delayering and imaging of the sample, face a tradeoff between throughput and resolution. Using focused ion beam (FIB) for delayering, although high precision, is low throughput. On the other hand, mechanical methods that can offer fast delayering, are low precision and may put the sample integrity at risk. Herein, we propose to use femtosecond laser ablation as a delayering method in combination with optical and confocal microscopy as the imaging technique for performing rapid 3D imaging. The use of confocal microscopy provides several advantages. First, it eliminates the 3D image distortion resulting from non-flat layers, caused by the difference in laser ablation rate of different materials. It further allows layer height variations to be maintained within a small range. Finally, it enables material characterization based on the processing of material ablation rate at different locations. The proposed method is applied on a printed circuit board (PCB), and the results are validated and compared with the X-ray CT image of the PCB part. |
format | Online Article Text |
id | pubmed-9296531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-92965312022-07-21 Rapid high-resolution volumetric imaging via laser ablation delayering and confocal imaging Phoulady, Adrian May, Nicholas Choi, Hongbin Suleiman, Yara Shahbazmohamadi, Sina Tavousi, Pouya Sci Rep Article Acquiring detailed 3D images of samples is needed for conducting thorough investigations in a wide range of applications. Doing so using nondestructive methods such as X-ray computed tomography (X-ray CT) has resolution limitations. Destructive methods, which work based on consecutive delayering and imaging of the sample, face a tradeoff between throughput and resolution. Using focused ion beam (FIB) for delayering, although high precision, is low throughput. On the other hand, mechanical methods that can offer fast delayering, are low precision and may put the sample integrity at risk. Herein, we propose to use femtosecond laser ablation as a delayering method in combination with optical and confocal microscopy as the imaging technique for performing rapid 3D imaging. The use of confocal microscopy provides several advantages. First, it eliminates the 3D image distortion resulting from non-flat layers, caused by the difference in laser ablation rate of different materials. It further allows layer height variations to be maintained within a small range. Finally, it enables material characterization based on the processing of material ablation rate at different locations. The proposed method is applied on a printed circuit board (PCB), and the results are validated and compared with the X-ray CT image of the PCB part. Nature Publishing Group UK 2022-07-19 /pmc/articles/PMC9296531/ /pubmed/35853990 http://dx.doi.org/10.1038/s41598-022-16519-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Phoulady, Adrian May, Nicholas Choi, Hongbin Suleiman, Yara Shahbazmohamadi, Sina Tavousi, Pouya Rapid high-resolution volumetric imaging via laser ablation delayering and confocal imaging |
title | Rapid high-resolution volumetric imaging via laser ablation delayering and confocal imaging |
title_full | Rapid high-resolution volumetric imaging via laser ablation delayering and confocal imaging |
title_fullStr | Rapid high-resolution volumetric imaging via laser ablation delayering and confocal imaging |
title_full_unstemmed | Rapid high-resolution volumetric imaging via laser ablation delayering and confocal imaging |
title_short | Rapid high-resolution volumetric imaging via laser ablation delayering and confocal imaging |
title_sort | rapid high-resolution volumetric imaging via laser ablation delayering and confocal imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296531/ https://www.ncbi.nlm.nih.gov/pubmed/35853990 http://dx.doi.org/10.1038/s41598-022-16519-2 |
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