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Fecal amine metabolite analysis before onset of severe necrotizing enterocolitis in preterm infants: a prospective case–control study

Infants developing necrotizing enterocolitis (NEC) have a different metabolomic profile compared to controls. The potential of specific metabolomics, i.e. amino acids and amino alcohols (AAA), as early diagnostic biomarkers for NEC is largely unexplored. In this multicenter prospective case–control...

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Autores principales: Deianova, N., el Manouni el Hassani, S., Struijs, E. A., Jansen, E. E. W., Bakkali, A., van de Wiel, M. A., de Boode, W. P., Hulzebos, C. V., van Kaam, A. H., Kramer, B. W., d’Haens, E., Vijlbrief, D. C., van Weissenbruch, M. M., de Jonge, W. J., Benninga, M. A., Niemarkt, H. J., de Boer, N. K. H., de Meij, T. G. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296556/
https://www.ncbi.nlm.nih.gov/pubmed/35853977
http://dx.doi.org/10.1038/s41598-022-16351-8
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author Deianova, N.
el Manouni el Hassani, S.
Struijs, E. A.
Jansen, E. E. W.
Bakkali, A.
van de Wiel, M. A.
de Boode, W. P.
Hulzebos, C. V.
van Kaam, A. H.
Kramer, B. W.
d’Haens, E.
Vijlbrief, D. C.
van Weissenbruch, M. M.
de Jonge, W. J.
Benninga, M. A.
Niemarkt, H. J.
de Boer, N. K. H.
de Meij, T. G. J.
author_facet Deianova, N.
el Manouni el Hassani, S.
Struijs, E. A.
Jansen, E. E. W.
Bakkali, A.
van de Wiel, M. A.
de Boode, W. P.
Hulzebos, C. V.
van Kaam, A. H.
Kramer, B. W.
d’Haens, E.
Vijlbrief, D. C.
van Weissenbruch, M. M.
de Jonge, W. J.
Benninga, M. A.
Niemarkt, H. J.
de Boer, N. K. H.
de Meij, T. G. J.
author_sort Deianova, N.
collection PubMed
description Infants developing necrotizing enterocolitis (NEC) have a different metabolomic profile compared to controls. The potential of specific metabolomics, i.e. amino acids and amino alcohols (AAA), as early diagnostic biomarkers for NEC is largely unexplored. In this multicenter prospective case–control study, longitudinally collected fecal samples from preterm infants (born <30 weeks of gestation) from 1–3 days before diagnosis of severe NEC (Bell’s stage IIIA/IIIB), were analyzed by targeted high-performance liquid chromatography (HPLC). Control samples were collected from gestational and postnatal age-matched infants. Thirty-one NEC cases (15 NEC IIIA;16 NEC IIIB) with 1:1 matched controls were included. Preclinical samples of infants with NEC were characterized by five increased essential amino acids—isoleucine, leucine, methionine, phenylalanine and valine. Lysine and ethanolamine ratios were lower prior to NEC, compared to control samples. A multivariate model was rendered based on isoleucine, lysine, ethanolamine, tryptophan and ornithine, modestly discriminating cases from controls (AUC 0.67; p < 0.001). Targeted HPLC pointed to several specific AAA alterations in samples collected 1–3 days before NEC onset, compared to controls. Whether this reflects metabolic alterations and has a role in early biomarker development for NEC, has yet to be elucidated.
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spelling pubmed-92965562022-07-21 Fecal amine metabolite analysis before onset of severe necrotizing enterocolitis in preterm infants: a prospective case–control study Deianova, N. el Manouni el Hassani, S. Struijs, E. A. Jansen, E. E. W. Bakkali, A. van de Wiel, M. A. de Boode, W. P. Hulzebos, C. V. van Kaam, A. H. Kramer, B. W. d’Haens, E. Vijlbrief, D. C. van Weissenbruch, M. M. de Jonge, W. J. Benninga, M. A. Niemarkt, H. J. de Boer, N. K. H. de Meij, T. G. J. Sci Rep Article Infants developing necrotizing enterocolitis (NEC) have a different metabolomic profile compared to controls. The potential of specific metabolomics, i.e. amino acids and amino alcohols (AAA), as early diagnostic biomarkers for NEC is largely unexplored. In this multicenter prospective case–control study, longitudinally collected fecal samples from preterm infants (born <30 weeks of gestation) from 1–3 days before diagnosis of severe NEC (Bell’s stage IIIA/IIIB), were analyzed by targeted high-performance liquid chromatography (HPLC). Control samples were collected from gestational and postnatal age-matched infants. Thirty-one NEC cases (15 NEC IIIA;16 NEC IIIB) with 1:1 matched controls were included. Preclinical samples of infants with NEC were characterized by five increased essential amino acids—isoleucine, leucine, methionine, phenylalanine and valine. Lysine and ethanolamine ratios were lower prior to NEC, compared to control samples. A multivariate model was rendered based on isoleucine, lysine, ethanolamine, tryptophan and ornithine, modestly discriminating cases from controls (AUC 0.67; p < 0.001). Targeted HPLC pointed to several specific AAA alterations in samples collected 1–3 days before NEC onset, compared to controls. Whether this reflects metabolic alterations and has a role in early biomarker development for NEC, has yet to be elucidated. Nature Publishing Group UK 2022-07-19 /pmc/articles/PMC9296556/ /pubmed/35853977 http://dx.doi.org/10.1038/s41598-022-16351-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Deianova, N.
el Manouni el Hassani, S.
Struijs, E. A.
Jansen, E. E. W.
Bakkali, A.
van de Wiel, M. A.
de Boode, W. P.
Hulzebos, C. V.
van Kaam, A. H.
Kramer, B. W.
d’Haens, E.
Vijlbrief, D. C.
van Weissenbruch, M. M.
de Jonge, W. J.
Benninga, M. A.
Niemarkt, H. J.
de Boer, N. K. H.
de Meij, T. G. J.
Fecal amine metabolite analysis before onset of severe necrotizing enterocolitis in preterm infants: a prospective case–control study
title Fecal amine metabolite analysis before onset of severe necrotizing enterocolitis in preterm infants: a prospective case–control study
title_full Fecal amine metabolite analysis before onset of severe necrotizing enterocolitis in preterm infants: a prospective case–control study
title_fullStr Fecal amine metabolite analysis before onset of severe necrotizing enterocolitis in preterm infants: a prospective case–control study
title_full_unstemmed Fecal amine metabolite analysis before onset of severe necrotizing enterocolitis in preterm infants: a prospective case–control study
title_short Fecal amine metabolite analysis before onset of severe necrotizing enterocolitis in preterm infants: a prospective case–control study
title_sort fecal amine metabolite analysis before onset of severe necrotizing enterocolitis in preterm infants: a prospective case–control study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296556/
https://www.ncbi.nlm.nih.gov/pubmed/35853977
http://dx.doi.org/10.1038/s41598-022-16351-8
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