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Grouping MWCNTs based on their similar potential to cause pulmonary hazard after inhalation: a case-study
BACKGROUND: The EU-project GRACIOUS developed an Integrated Approach to Testing and Assessment (IATA) to support grouping high aspect ratio nanomaterials (HARNs) presenting a similar inhalation hazard. Application of grouping reduces the need to assess toxicity on a case-by-case basis and supports r...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297605/ https://www.ncbi.nlm.nih.gov/pubmed/35854357 http://dx.doi.org/10.1186/s12989-022-00487-6 |
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author | Murphy, Fiona Jacobsen, Nicklas Raun Di Ianni, Emilio Johnston, Helinor Braakhuis, Hedwig Peijnenburg, Willie Oomen, Agnes Fernandes, Teresa Stone, Vicki |
author_facet | Murphy, Fiona Jacobsen, Nicklas Raun Di Ianni, Emilio Johnston, Helinor Braakhuis, Hedwig Peijnenburg, Willie Oomen, Agnes Fernandes, Teresa Stone, Vicki |
author_sort | Murphy, Fiona |
collection | PubMed |
description | BACKGROUND: The EU-project GRACIOUS developed an Integrated Approach to Testing and Assessment (IATA) to support grouping high aspect ratio nanomaterials (HARNs) presenting a similar inhalation hazard. Application of grouping reduces the need to assess toxicity on a case-by-case basis and supports read-across of hazard data from substances that have the data required for risk assessment (source) to those that lack such data (target). The HARN IATA, based on the fibre paradigm for pathogenic fibres, facilitates structured data gathering to propose groups of similar HARN and to support read-across by prompting users to address relevant questions regarding HARN morphology, biopersistence and inflammatory potential. The IATA is structured in tiers, allowing grouping decisions to be made using simple in vitro or in silico methods in Tier1 progressing to in vivo approaches at the highest Tier3. Here we present a case-study testing the applicability of GRACIOUS IATA to form an evidence-based group of multiwalled carbon nanotubes (MWCNT) posing a similar predicted fibre-hazard, to support read-across and reduce the burden of toxicity testing. RESULTS: The case-study uses data on 15 different MWCNT, obtained from the published literature. By following the IATA, a group of 2 MWCNT was identified (NRCWE006 and NM-401) based on a high degree of similarity. A pairwise similarity assessment was subsequently conducted between the grouped MWCNT to evaluate the potential to conduct read-across and fill data gaps required for regulatory hazard assessment. The similarity assessment, based on expert judgement of Tier 1 assay results, predicts both MWCNT are likely to cause a similar acute in vivo hazard. This result supports the possibility for read-across of sub-chronic and chronic hazard endpoint data for lung fibrosis and carcinogenicity between the 2 grouped MWCNT. The implications of accepting the similarity assessment based on expert judgement of the MWCNT group are considered to stimulate future discussion on the level of similarity between group members considered sufficient to allow regulatory acceptance of a read-across argument. CONCLUSION: This proof-of-concept case-study demonstrates how a grouping hypothesis and IATA may be used to support a nuanced and evidence-based grouping of ‘similar’ MWCNT and the subsequent interpolation of data between group members to streamline the hazard assessment process. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12989-022-00487-6. |
format | Online Article Text |
id | pubmed-9297605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-92976052022-07-21 Grouping MWCNTs based on their similar potential to cause pulmonary hazard after inhalation: a case-study Murphy, Fiona Jacobsen, Nicklas Raun Di Ianni, Emilio Johnston, Helinor Braakhuis, Hedwig Peijnenburg, Willie Oomen, Agnes Fernandes, Teresa Stone, Vicki Part Fibre Toxicol Research BACKGROUND: The EU-project GRACIOUS developed an Integrated Approach to Testing and Assessment (IATA) to support grouping high aspect ratio nanomaterials (HARNs) presenting a similar inhalation hazard. Application of grouping reduces the need to assess toxicity on a case-by-case basis and supports read-across of hazard data from substances that have the data required for risk assessment (source) to those that lack such data (target). The HARN IATA, based on the fibre paradigm for pathogenic fibres, facilitates structured data gathering to propose groups of similar HARN and to support read-across by prompting users to address relevant questions regarding HARN morphology, biopersistence and inflammatory potential. The IATA is structured in tiers, allowing grouping decisions to be made using simple in vitro or in silico methods in Tier1 progressing to in vivo approaches at the highest Tier3. Here we present a case-study testing the applicability of GRACIOUS IATA to form an evidence-based group of multiwalled carbon nanotubes (MWCNT) posing a similar predicted fibre-hazard, to support read-across and reduce the burden of toxicity testing. RESULTS: The case-study uses data on 15 different MWCNT, obtained from the published literature. By following the IATA, a group of 2 MWCNT was identified (NRCWE006 and NM-401) based on a high degree of similarity. A pairwise similarity assessment was subsequently conducted between the grouped MWCNT to evaluate the potential to conduct read-across and fill data gaps required for regulatory hazard assessment. The similarity assessment, based on expert judgement of Tier 1 assay results, predicts both MWCNT are likely to cause a similar acute in vivo hazard. This result supports the possibility for read-across of sub-chronic and chronic hazard endpoint data for lung fibrosis and carcinogenicity between the 2 grouped MWCNT. The implications of accepting the similarity assessment based on expert judgement of the MWCNT group are considered to stimulate future discussion on the level of similarity between group members considered sufficient to allow regulatory acceptance of a read-across argument. CONCLUSION: This proof-of-concept case-study demonstrates how a grouping hypothesis and IATA may be used to support a nuanced and evidence-based grouping of ‘similar’ MWCNT and the subsequent interpolation of data between group members to streamline the hazard assessment process. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12989-022-00487-6. BioMed Central 2022-07-20 /pmc/articles/PMC9297605/ /pubmed/35854357 http://dx.doi.org/10.1186/s12989-022-00487-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Murphy, Fiona Jacobsen, Nicklas Raun Di Ianni, Emilio Johnston, Helinor Braakhuis, Hedwig Peijnenburg, Willie Oomen, Agnes Fernandes, Teresa Stone, Vicki Grouping MWCNTs based on their similar potential to cause pulmonary hazard after inhalation: a case-study |
title | Grouping MWCNTs based on their similar potential to cause pulmonary hazard after inhalation: a case-study |
title_full | Grouping MWCNTs based on their similar potential to cause pulmonary hazard after inhalation: a case-study |
title_fullStr | Grouping MWCNTs based on their similar potential to cause pulmonary hazard after inhalation: a case-study |
title_full_unstemmed | Grouping MWCNTs based on their similar potential to cause pulmonary hazard after inhalation: a case-study |
title_short | Grouping MWCNTs based on their similar potential to cause pulmonary hazard after inhalation: a case-study |
title_sort | grouping mwcnts based on their similar potential to cause pulmonary hazard after inhalation: a case-study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297605/ https://www.ncbi.nlm.nih.gov/pubmed/35854357 http://dx.doi.org/10.1186/s12989-022-00487-6 |
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