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Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification

Recently, Copan (Italy) introduced the Colibrí instrument for automated colony picking and preparation of matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) target plates. Our study aimed to validate this system for yeasts as such testing has not been performed yet and is a missi...

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Autores principales: Heestermans, Robbe, Herroelen, Pauline, Emmerechts, Kristof, Vandoorslaer, Kristof, De Geyter, Deborah, Demuyser, Thomas, Wybo, Ingrid, Piérard, Denis, Muyldermans, Astrid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297811/
https://www.ncbi.nlm.nih.gov/pubmed/35703578
http://dx.doi.org/10.1128/jcm.00237-22
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author Heestermans, Robbe
Herroelen, Pauline
Emmerechts, Kristof
Vandoorslaer, Kristof
De Geyter, Deborah
Demuyser, Thomas
Wybo, Ingrid
Piérard, Denis
Muyldermans, Astrid
author_facet Heestermans, Robbe
Herroelen, Pauline
Emmerechts, Kristof
Vandoorslaer, Kristof
De Geyter, Deborah
Demuyser, Thomas
Wybo, Ingrid
Piérard, Denis
Muyldermans, Astrid
author_sort Heestermans, Robbe
collection PubMed
description Recently, Copan (Italy) introduced the Colibrí instrument for automated colony picking and preparation of matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) target plates. Our study aimed to validate this system for yeasts as such testing has not been performed yet and is a missing link needed to implement the system for routine use. Fifty-five Candida strains were selected to evaluate the accuracy of Colibrí. For each strain, a sheep blood agar plate supplemented with X and V factors (HEM) and a Sabouraud agar plate (SAB) were inoculated and incubated using the WASPlab specimen processing system (Copan). After 18 h and 36 h of incubation, the isolates were spotted in parallel using Colibrí and manually onto MALDI-TOF target plates with the addition of formic acid and identified using MALDI-TOF mass spectrometry. The reproducibility was evaluated using ATCC reference and clinical isolate-derived strains. The cumulative percentage of acceptable identification scores (IDs) after 36 h was 91% for strains cultured on HEM plates using both Colibrí and the manual method. The SAB plates showed inferior results for both Colibrí (76%) and the manual method (78%). We observed an overall agreement of 92% at 18 h for identification of the strains on the HEM plates between Colibrí and the manual method and 94% after 36 h. For the SAB plates, the agreement was 78% after 18 h and 84% after 36 h. Apart from Candida dubliniensis and Candida tropicalis, all Candida species were identified with 100% accuracy using Colibrí on HEM plates. We observed good agreement between Colibrí and the manual reference method. These results demonstrate that Colibrí is a reliable system for MALDI-TOF target preparation for yeast identification, allowing increased standardization and less hands-on time.
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spelling pubmed-92978112022-07-21 Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification Heestermans, Robbe Herroelen, Pauline Emmerechts, Kristof Vandoorslaer, Kristof De Geyter, Deborah Demuyser, Thomas Wybo, Ingrid Piérard, Denis Muyldermans, Astrid J Clin Microbiol Mycology Recently, Copan (Italy) introduced the Colibrí instrument for automated colony picking and preparation of matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) target plates. Our study aimed to validate this system for yeasts as such testing has not been performed yet and is a missing link needed to implement the system for routine use. Fifty-five Candida strains were selected to evaluate the accuracy of Colibrí. For each strain, a sheep blood agar plate supplemented with X and V factors (HEM) and a Sabouraud agar plate (SAB) were inoculated and incubated using the WASPlab specimen processing system (Copan). After 18 h and 36 h of incubation, the isolates were spotted in parallel using Colibrí and manually onto MALDI-TOF target plates with the addition of formic acid and identified using MALDI-TOF mass spectrometry. The reproducibility was evaluated using ATCC reference and clinical isolate-derived strains. The cumulative percentage of acceptable identification scores (IDs) after 36 h was 91% for strains cultured on HEM plates using both Colibrí and the manual method. The SAB plates showed inferior results for both Colibrí (76%) and the manual method (78%). We observed an overall agreement of 92% at 18 h for identification of the strains on the HEM plates between Colibrí and the manual method and 94% after 36 h. For the SAB plates, the agreement was 78% after 18 h and 84% after 36 h. Apart from Candida dubliniensis and Candida tropicalis, all Candida species were identified with 100% accuracy using Colibrí on HEM plates. We observed good agreement between Colibrí and the manual reference method. These results demonstrate that Colibrí is a reliable system for MALDI-TOF target preparation for yeast identification, allowing increased standardization and less hands-on time. American Society for Microbiology 2022-06-15 /pmc/articles/PMC9297811/ /pubmed/35703578 http://dx.doi.org/10.1128/jcm.00237-22 Text en Copyright © 2022 Heestermans et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Mycology
Heestermans, Robbe
Herroelen, Pauline
Emmerechts, Kristof
Vandoorslaer, Kristof
De Geyter, Deborah
Demuyser, Thomas
Wybo, Ingrid
Piérard, Denis
Muyldermans, Astrid
Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification
title Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification
title_full Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification
title_fullStr Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification
title_full_unstemmed Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification
title_short Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification
title_sort validation of the colibrí instrument for automated preparation of maldi-tof ms targets for yeast identification
topic Mycology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297811/
https://www.ncbi.nlm.nih.gov/pubmed/35703578
http://dx.doi.org/10.1128/jcm.00237-22
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