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Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification
Recently, Copan (Italy) introduced the Colibrí instrument for automated colony picking and preparation of matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) target plates. Our study aimed to validate this system for yeasts as such testing has not been performed yet and is a missi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297811/ https://www.ncbi.nlm.nih.gov/pubmed/35703578 http://dx.doi.org/10.1128/jcm.00237-22 |
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author | Heestermans, Robbe Herroelen, Pauline Emmerechts, Kristof Vandoorslaer, Kristof De Geyter, Deborah Demuyser, Thomas Wybo, Ingrid Piérard, Denis Muyldermans, Astrid |
author_facet | Heestermans, Robbe Herroelen, Pauline Emmerechts, Kristof Vandoorslaer, Kristof De Geyter, Deborah Demuyser, Thomas Wybo, Ingrid Piérard, Denis Muyldermans, Astrid |
author_sort | Heestermans, Robbe |
collection | PubMed |
description | Recently, Copan (Italy) introduced the Colibrí instrument for automated colony picking and preparation of matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) target plates. Our study aimed to validate this system for yeasts as such testing has not been performed yet and is a missing link needed to implement the system for routine use. Fifty-five Candida strains were selected to evaluate the accuracy of Colibrí. For each strain, a sheep blood agar plate supplemented with X and V factors (HEM) and a Sabouraud agar plate (SAB) were inoculated and incubated using the WASPlab specimen processing system (Copan). After 18 h and 36 h of incubation, the isolates were spotted in parallel using Colibrí and manually onto MALDI-TOF target plates with the addition of formic acid and identified using MALDI-TOF mass spectrometry. The reproducibility was evaluated using ATCC reference and clinical isolate-derived strains. The cumulative percentage of acceptable identification scores (IDs) after 36 h was 91% for strains cultured on HEM plates using both Colibrí and the manual method. The SAB plates showed inferior results for both Colibrí (76%) and the manual method (78%). We observed an overall agreement of 92% at 18 h for identification of the strains on the HEM plates between Colibrí and the manual method and 94% after 36 h. For the SAB plates, the agreement was 78% after 18 h and 84% after 36 h. Apart from Candida dubliniensis and Candida tropicalis, all Candida species were identified with 100% accuracy using Colibrí on HEM plates. We observed good agreement between Colibrí and the manual reference method. These results demonstrate that Colibrí is a reliable system for MALDI-TOF target preparation for yeast identification, allowing increased standardization and less hands-on time. |
format | Online Article Text |
id | pubmed-9297811 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-92978112022-07-21 Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification Heestermans, Robbe Herroelen, Pauline Emmerechts, Kristof Vandoorslaer, Kristof De Geyter, Deborah Demuyser, Thomas Wybo, Ingrid Piérard, Denis Muyldermans, Astrid J Clin Microbiol Mycology Recently, Copan (Italy) introduced the Colibrí instrument for automated colony picking and preparation of matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) target plates. Our study aimed to validate this system for yeasts as such testing has not been performed yet and is a missing link needed to implement the system for routine use. Fifty-five Candida strains were selected to evaluate the accuracy of Colibrí. For each strain, a sheep blood agar plate supplemented with X and V factors (HEM) and a Sabouraud agar plate (SAB) were inoculated and incubated using the WASPlab specimen processing system (Copan). After 18 h and 36 h of incubation, the isolates were spotted in parallel using Colibrí and manually onto MALDI-TOF target plates with the addition of formic acid and identified using MALDI-TOF mass spectrometry. The reproducibility was evaluated using ATCC reference and clinical isolate-derived strains. The cumulative percentage of acceptable identification scores (IDs) after 36 h was 91% for strains cultured on HEM plates using both Colibrí and the manual method. The SAB plates showed inferior results for both Colibrí (76%) and the manual method (78%). We observed an overall agreement of 92% at 18 h for identification of the strains on the HEM plates between Colibrí and the manual method and 94% after 36 h. For the SAB plates, the agreement was 78% after 18 h and 84% after 36 h. Apart from Candida dubliniensis and Candida tropicalis, all Candida species were identified with 100% accuracy using Colibrí on HEM plates. We observed good agreement between Colibrí and the manual reference method. These results demonstrate that Colibrí is a reliable system for MALDI-TOF target preparation for yeast identification, allowing increased standardization and less hands-on time. American Society for Microbiology 2022-06-15 /pmc/articles/PMC9297811/ /pubmed/35703578 http://dx.doi.org/10.1128/jcm.00237-22 Text en Copyright © 2022 Heestermans et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Mycology Heestermans, Robbe Herroelen, Pauline Emmerechts, Kristof Vandoorslaer, Kristof De Geyter, Deborah Demuyser, Thomas Wybo, Ingrid Piérard, Denis Muyldermans, Astrid Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification |
title | Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification |
title_full | Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification |
title_fullStr | Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification |
title_full_unstemmed | Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification |
title_short | Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification |
title_sort | validation of the colibrí instrument for automated preparation of maldi-tof ms targets for yeast identification |
topic | Mycology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297811/ https://www.ncbi.nlm.nih.gov/pubmed/35703578 http://dx.doi.org/10.1128/jcm.00237-22 |
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