Cargando…

RNase H-based analysis of synthetic mRNA 5′ cap incorporation

Advances in mRNA synthesis and lipid nanoparticles technologies have helped make mRNA therapeutics and vaccines a reality. The 5′ cap structure is a crucial modification required to functionalize synthetic mRNA for efficient protein translation in vivo and evasion of cellular innate immune responses...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chan, S. Hong, Whipple, Joseph M., Dai, Nan, Kelley, Theresa M., Withers, Kathryn, Tzertzinis, George, Corrêa, Ivan R., Robb, G. Brett
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2022
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297845/ https://www.ncbi.nlm.nih.gov/pubmed/35680168 http://dx.doi.org/10.1261/rna.079173.122

Ejemplares similares

Human RNase 4 improves mRNA sequence characterization by LC–MS/MS
por: Wolf, Eric J, et al.
Publicado: (2022)

Biochemical characterization of mRNA capping enzyme from Faustovirus
por: Chan, S. Hong, et al.
Publicado: (2023)

mRNA capping: biological functions and applications
por: Ramanathan, Anand, et al.
Publicado: (2016)

Chemical capping improves template switching and enhances sequencing of small RNAs
por: Wulf, Madalee G, et al.
Publicado: (2021)

Synthetic mRNA capping
por: Muttach, Fabian, et al.
Publicado: (2017)

CAP-MAP: cap analysis protocol with minimal analyte processing, a rapid and sensitive approach to analysing mRNA cap structures
por: Galloway, Alison, et al.
Publicado: (2020)

“NAD-capQ” detection and quantitation of NAD caps
por: Grudzien-Nogalska, Ewa, et al.
Publicado: (2018)

Comprehensive determination of transcription start sites derived from all RNA polymerases using ReCappable-seq
por: Yan, Bo, et al.
Publicado: (2022)

Low-input RNase footprinting for simultaneous quantification of cytosolic and mitochondrial translation
por: Li, Qianru, et al.
Publicado: (2022)

Characterization of RNase R-digested cellular RNA source that consists of lariat and circular RNAs from pre-mRNA splicing
por: Suzuki, Hitoshi, et al.
Publicado: (2006)

RNase-mediated protein footprint sequencing reveals protein-binding sites throughout the human transcriptome
por: Silverman, Ian M, et al.
Publicado: (2014)

A cap 0-dependent mRNA capture method to analyze the yeast transcriptome
por: Nowacka, Martyna, et al.
Publicado: (2022)

FFPEcap-seq: a method for sequencing capped RNAs in formalin-fixed paraffin-embedded samples
por: Vahrenkamp, Jeffery M., et al.
Publicado: (2019)

E. coli RNase I exhibits a strong Ca(2+)-dependent inherent double-stranded RNase activity
por: Grünberg, Sebastian, et al.
Publicado: (2021)

The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping
por: Wulf, Madalee G., et al.
Publicado: (2019)

Argonaute-based programmable RNase as a tool for cleavage of highly-structured RNA
por: Dayeh, Daniel M, et al.
Publicado: (2018)

X-CAP improves pathogenicity prediction of stopgain variants
por: Rastogi, Ruchir, et al.
Publicado: (2022)

Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells
por: Kawasaki, Shunsuke, et al.
Publicado: (2017)

Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA
por: Wang, Jin, et al.
Publicado: (2019)

Identification of RNase-sensitive LINE-1 Ribonucleoprotein Interactionsby Differential Affinity Immobilization
por: Jiang, Hua, et al.
Publicado: (2019)

Compatible Pollinations in Solanum chacoense Decrease Both S-RNase and S-RNase mRNA
por: Liu, Bolin, et al.
Publicado: (2009)

Improvement of RNA secondary structure prediction using RNase H cleavage and randomized oligonucleotides
por: Kauffmann, Andrew D., et al.
Publicado: (2009)

Defining the 5΄ and 3΄ landscape of the Drosophila transcriptome with Exo-seq and RNaseH-seq
por: Afik, Shaked, et al.
Publicado: (2017)

Deep sequencing of short capped RNAs reveals novel families of noncoding RNAs
por: de Hoon, Michiel, et al.
Publicado: (2022)

Viperin mRNA is a novel target for the human RNase MRP/RNase P endoribonuclease
por: Mattijssen, Sandy, et al.
Publicado: (2010)

Enzymatic characterization of mRNA cap adenosine-N6 methyltransferase PCIF1 activity on uncapped RNAs
por: Yu, Dan, et al.
Publicado: (2022)

Post-synthetic benzylation of the mRNA 5′ cap via enzymatic cascade reactions
por: Cornelissen, N. V., et al.
Publicado: (2023)

capCLIP: a new tool to probe translational control in human cells through capture and identification of the eIF4E–mRNA interactome
por: Jensen, Kirk B, et al.
Publicado: (2021)

Versatile strategy using vaccinia virus-capping enzyme to synthesize functional 5′ cap-modified mRNAs
por: Ohno, Hirohisa, et al.
Publicado: (2023)

CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts
por: Katayama, Shintaro, et al.
Publicado: (2007)

A general method for rapid and cost-efficient large-scale production of 5′ capped RNA
por: Fuchs, Anna-Lisa, et al.
Publicado: (2016)

Cap-Independent mRNA Translation in Germ Cells
por: Keiper, Brett D.
Publicado: (2019)

Sequence verification of synthetic DNA by assembly of sequencing reads
por: Wilson, Mandy L., et al.
Publicado: (2013)

A convenient strategy to clone small RNA and mRNA for high-throughput sequencing
por: Li, Lichao, et al.
Publicado: (2020)

ONE-seq: epitranscriptome and gene-specific profiling of NAD-capped RNA
por: Niu, Kongyan, et al.
Publicado: (2022)

Dissection of complex adult traits in a mouse synthetic population
por: Burke, David T., et al.
Publicado: (2012)

CapR: revealing structural specificities of RNA-binding protein target recognition using CLIP-seq data
por: Fukunaga, Tsukasa, et al.
Publicado: (2014)

Detecting DNA regulatory motifs by incorporating positional trends in information content
por: Kechris, Katherina J, et al.
Publicado: (2004)

Impact of RNase E and RNase J on Global mRNA Metabolism in the Cyanobacterium Synechocystis PCC6803
por: Cavaiuolo, Marina, et al.
Publicado: (2020)

DeepCAGE: Incorporating Transcription Factors in Genome-wide Prediction of Chromatin Accessibility
por: Liu, Qiao, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_9pm89a5nualvb207g78h3tlrbg