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Determination of nutrient content, β-carotene, and antioxidant activity of Moringa oleifera extraction using organic solution

OBJECTIVE: The research was conducted to determine β-carotene and antioxidant activities and screening of phytochemical substances of Moringa oleifera extraction using organic solution. MATERIALS AND METHODS: 550 gm of M. oliefera leaf flour was macerated. This research was conducted by laboratory e...

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Autores principales: Haroen, Ucop, Syafwan, Kurniawan, Kiki, Budiansyah, Agus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET) 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298099/
https://www.ncbi.nlm.nih.gov/pubmed/35891658
http://dx.doi.org/10.5455/javar.2022.i590
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author Haroen, Ucop
Syafwan,
Kurniawan, Kiki
Budiansyah, Agus
author_facet Haroen, Ucop
Syafwan,
Kurniawan, Kiki
Budiansyah, Agus
author_sort Haroen, Ucop
collection PubMed
description OBJECTIVE: The research was conducted to determine β-carotene and antioxidant activities and screening of phytochemical substances of Moringa oleifera extraction using organic solution. MATERIALS AND METHODS: 550 gm of M. oliefera leaf flour was macerated. This research was conducted by laboratory experiments using the maceration method. The extraction was performed using three kinds of solvents, which are n-hexane, ethyl acetate, and methanol; for 3 x 24 h, they were concentrated with a rotary evaporator. Then, the flavonoid, phenolic, β-carotene isolation, and antioxidant tests were conducted using the 2,2-diphenyl-1-picrylhydrazyl on each fraction (n-hexane, ethyl acetate, and methanol). RESULTS: The results of weighing each concentrated extract from the maceration process of each fraction (n-hexane, ethyl acetate, and methanol) were 12.67, 35.67, and 49.29 gm, with the total phenolic content (1.4595 ± 0.361, 46.5489 ± 1.832, and 39.74574 ± 0.786) and total flavonoid content of each fraction (3.3056 ± 0.039, 58.6389 ± 2.051, and 48.9056 ± 0.0809), respectively. The antioxidant activity test on the crude extract from the ethyl acetate fractionation showed that the IC(50) value was 30.309 mg/ml. The ethyl acetate fraction has a high total phenolic and flavonoid content. The results of the isolation of β-carotene from M. oleifera leaf flour were 0.4798 gm, or equivalent to 0.956% carotenoids. CONCLUSIONS: Based on the results of the research, M. oleifera leaves are identified to have a fairly high antioxidant activity, which is 30.309 mg/ml, resulting from the potential compounds in M. oleifera leaves that function as inhibitors of antioxidant activity, which are the groups of phenolic and flavonoid compounds.
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spelling pubmed-92980992022-07-25 Determination of nutrient content, β-carotene, and antioxidant activity of Moringa oleifera extraction using organic solution Haroen, Ucop Syafwan, Kurniawan, Kiki Budiansyah, Agus J Adv Vet Anim Res Original Article OBJECTIVE: The research was conducted to determine β-carotene and antioxidant activities and screening of phytochemical substances of Moringa oleifera extraction using organic solution. MATERIALS AND METHODS: 550 gm of M. oliefera leaf flour was macerated. This research was conducted by laboratory experiments using the maceration method. The extraction was performed using three kinds of solvents, which are n-hexane, ethyl acetate, and methanol; for 3 x 24 h, they were concentrated with a rotary evaporator. Then, the flavonoid, phenolic, β-carotene isolation, and antioxidant tests were conducted using the 2,2-diphenyl-1-picrylhydrazyl on each fraction (n-hexane, ethyl acetate, and methanol). RESULTS: The results of weighing each concentrated extract from the maceration process of each fraction (n-hexane, ethyl acetate, and methanol) were 12.67, 35.67, and 49.29 gm, with the total phenolic content (1.4595 ± 0.361, 46.5489 ± 1.832, and 39.74574 ± 0.786) and total flavonoid content of each fraction (3.3056 ± 0.039, 58.6389 ± 2.051, and 48.9056 ± 0.0809), respectively. The antioxidant activity test on the crude extract from the ethyl acetate fractionation showed that the IC(50) value was 30.309 mg/ml. The ethyl acetate fraction has a high total phenolic and flavonoid content. The results of the isolation of β-carotene from M. oleifera leaf flour were 0.4798 gm, or equivalent to 0.956% carotenoids. CONCLUSIONS: Based on the results of the research, M. oleifera leaves are identified to have a fairly high antioxidant activity, which is 30.309 mg/ml, resulting from the potential compounds in M. oleifera leaves that function as inhibitors of antioxidant activity, which are the groups of phenolic and flavonoid compounds. A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET) 2022-06-26 /pmc/articles/PMC9298099/ /pubmed/35891658 http://dx.doi.org/10.5455/javar.2022.i590 Text en Copyright: © Journal of Advanced Veterinary and Animal Research https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) )
spellingShingle Original Article
Haroen, Ucop
Syafwan,
Kurniawan, Kiki
Budiansyah, Agus
Determination of nutrient content, β-carotene, and antioxidant activity of Moringa oleifera extraction using organic solution
title Determination of nutrient content, β-carotene, and antioxidant activity of Moringa oleifera extraction using organic solution
title_full Determination of nutrient content, β-carotene, and antioxidant activity of Moringa oleifera extraction using organic solution
title_fullStr Determination of nutrient content, β-carotene, and antioxidant activity of Moringa oleifera extraction using organic solution
title_full_unstemmed Determination of nutrient content, β-carotene, and antioxidant activity of Moringa oleifera extraction using organic solution
title_short Determination of nutrient content, β-carotene, and antioxidant activity of Moringa oleifera extraction using organic solution
title_sort determination of nutrient content, β-carotene, and antioxidant activity of moringa oleifera extraction using organic solution
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298099/
https://www.ncbi.nlm.nih.gov/pubmed/35891658
http://dx.doi.org/10.5455/javar.2022.i590
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