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Overexpression approaches to advance understanding of Candida albicans

Candida albicans is an opportunistic fungal pathogen that is responsible for infections linked to high mortality. Loss‐of‐function approaches, taking advantage of gene knockouts or inducible down‐regulation, have been successfully used in this species in order to understand gene function. However, o...

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Autores principales: Rai, Laxmi Shanker, van Wijlick, Lasse, Chauvel, Murielle, d’Enfert, Christophe, Legrand, Mélanie, Bachellier‐Bassi, Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298300/
https://www.ncbi.nlm.nih.gov/pubmed/34569668
http://dx.doi.org/10.1111/mmi.14818
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author Rai, Laxmi Shanker
van Wijlick, Lasse
Chauvel, Murielle
d’Enfert, Christophe
Legrand, Mélanie
Bachellier‐Bassi, Sophie
author_facet Rai, Laxmi Shanker
van Wijlick, Lasse
Chauvel, Murielle
d’Enfert, Christophe
Legrand, Mélanie
Bachellier‐Bassi, Sophie
author_sort Rai, Laxmi Shanker
collection PubMed
description Candida albicans is an opportunistic fungal pathogen that is responsible for infections linked to high mortality. Loss‐of‐function approaches, taking advantage of gene knockouts or inducible down‐regulation, have been successfully used in this species in order to understand gene function. However, overexpression of a gene provides an alternative, powerful tool to elucidate gene function and identify novel phenotypes. Notably, overexpression can identify pathway components that might remain undetected using loss‐of‐function approaches. Several repressible or inducible promoters have been developed which allow to shut off or turn on the expression of a gene in C. albicans upon growth in the presence of a repressor or inducer. In this review, we summarize recent overexpression approaches used to study different aspects of C. albicans biology, including morphogenesis, biofilm formation, drug tolerance, and commensalism.
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spelling pubmed-92983002022-07-21 Overexpression approaches to advance understanding of Candida albicans Rai, Laxmi Shanker van Wijlick, Lasse Chauvel, Murielle d’Enfert, Christophe Legrand, Mélanie Bachellier‐Bassi, Sophie Mol Microbiol Microreviews Candida albicans is an opportunistic fungal pathogen that is responsible for infections linked to high mortality. Loss‐of‐function approaches, taking advantage of gene knockouts or inducible down‐regulation, have been successfully used in this species in order to understand gene function. However, overexpression of a gene provides an alternative, powerful tool to elucidate gene function and identify novel phenotypes. Notably, overexpression can identify pathway components that might remain undetected using loss‐of‐function approaches. Several repressible or inducible promoters have been developed which allow to shut off or turn on the expression of a gene in C. albicans upon growth in the presence of a repressor or inducer. In this review, we summarize recent overexpression approaches used to study different aspects of C. albicans biology, including morphogenesis, biofilm formation, drug tolerance, and commensalism. John Wiley and Sons Inc. 2021-10-18 2022-03 /pmc/articles/PMC9298300/ /pubmed/34569668 http://dx.doi.org/10.1111/mmi.14818 Text en © 2021 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Microreviews
Rai, Laxmi Shanker
van Wijlick, Lasse
Chauvel, Murielle
d’Enfert, Christophe
Legrand, Mélanie
Bachellier‐Bassi, Sophie
Overexpression approaches to advance understanding of Candida albicans
title Overexpression approaches to advance understanding of Candida albicans
title_full Overexpression approaches to advance understanding of Candida albicans
title_fullStr Overexpression approaches to advance understanding of Candida albicans
title_full_unstemmed Overexpression approaches to advance understanding of Candida albicans
title_short Overexpression approaches to advance understanding of Candida albicans
title_sort overexpression approaches to advance understanding of candida albicans
topic Microreviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298300/
https://www.ncbi.nlm.nih.gov/pubmed/34569668
http://dx.doi.org/10.1111/mmi.14818
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