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The rapid and sensitive detection of edible bird’s nest (Aerodramus fuciphagus) in processed food by a loop-mediated isothermal amplification (LAMP) assay

Edible bird’s nest (EBN) is a well-known and precious traditional Chinese herbal material (CHM). Because of this, preventing the adulteration of EBN efficiently and precisely is crucial to protect consumers’ interests and health. In this study, a loop-mediated isothermal amplification (LAMP) assay w...

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Autores principales: Lee, Meng-Shiou, Huang, Jhong-Yong, Lien, Yi-Yang, Sheu, Shyang-Chwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298639/
https://www.ncbi.nlm.nih.gov/pubmed/30648568
http://dx.doi.org/10.1016/j.jfda.2018.08.003
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author Lee, Meng-Shiou
Huang, Jhong-Yong
Lien, Yi-Yang
Sheu, Shyang-Chwen
author_facet Lee, Meng-Shiou
Huang, Jhong-Yong
Lien, Yi-Yang
Sheu, Shyang-Chwen
author_sort Lee, Meng-Shiou
collection PubMed
description Edible bird’s nest (EBN) is a well-known and precious traditional Chinese herbal material (CHM). Because of this, preventing the adulteration of EBN efficiently and precisely is crucial to protect consumers’ interests and health. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for the detection of EBN using specifically designed LAMP primers. The results demonstrated that the identification of EBN by LAMP assay was specific and rapid (within 1 h). It had no cross-reaction with EBN adulterants, including white fungus, egg white and pig skin, in different ratios. The relative detection limit was 0.01% EBN in the adulterants. Moreover, the sensitivity of LAMP in authenticating EBN was 10(−8) μg, it showed higher sensitivity than that of conventional PCR with 10(5) fold. When genomic DNAs extracted from boiled or steamed EBN samples were used as templates, LAMP for EBN detection was not affected and was reproducible after heat processing. In conclusion, the LAMP assay established herein could be applicable for authenticating EBN and for identifying commercial EBN products in herbal markets.
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spelling pubmed-92986392022-08-09 The rapid and sensitive detection of edible bird’s nest (Aerodramus fuciphagus) in processed food by a loop-mediated isothermal amplification (LAMP) assay Lee, Meng-Shiou Huang, Jhong-Yong Lien, Yi-Yang Sheu, Shyang-Chwen J Food Drug Anal Original Article Edible bird’s nest (EBN) is a well-known and precious traditional Chinese herbal material (CHM). Because of this, preventing the adulteration of EBN efficiently and precisely is crucial to protect consumers’ interests and health. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for the detection of EBN using specifically designed LAMP primers. The results demonstrated that the identification of EBN by LAMP assay was specific and rapid (within 1 h). It had no cross-reaction with EBN adulterants, including white fungus, egg white and pig skin, in different ratios. The relative detection limit was 0.01% EBN in the adulterants. Moreover, the sensitivity of LAMP in authenticating EBN was 10(−8) μg, it showed higher sensitivity than that of conventional PCR with 10(5) fold. When genomic DNAs extracted from boiled or steamed EBN samples were used as templates, LAMP for EBN detection was not affected and was reproducible after heat processing. In conclusion, the LAMP assay established herein could be applicable for authenticating EBN and for identifying commercial EBN products in herbal markets. Taiwan Food and Drug Administration 2018-08-24 /pmc/articles/PMC9298639/ /pubmed/30648568 http://dx.doi.org/10.1016/j.jfda.2018.08.003 Text en © 2019 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Lee, Meng-Shiou
Huang, Jhong-Yong
Lien, Yi-Yang
Sheu, Shyang-Chwen
The rapid and sensitive detection of edible bird’s nest (Aerodramus fuciphagus) in processed food by a loop-mediated isothermal amplification (LAMP) assay
title The rapid and sensitive detection of edible bird’s nest (Aerodramus fuciphagus) in processed food by a loop-mediated isothermal amplification (LAMP) assay
title_full The rapid and sensitive detection of edible bird’s nest (Aerodramus fuciphagus) in processed food by a loop-mediated isothermal amplification (LAMP) assay
title_fullStr The rapid and sensitive detection of edible bird’s nest (Aerodramus fuciphagus) in processed food by a loop-mediated isothermal amplification (LAMP) assay
title_full_unstemmed The rapid and sensitive detection of edible bird’s nest (Aerodramus fuciphagus) in processed food by a loop-mediated isothermal amplification (LAMP) assay
title_short The rapid and sensitive detection of edible bird’s nest (Aerodramus fuciphagus) in processed food by a loop-mediated isothermal amplification (LAMP) assay
title_sort rapid and sensitive detection of edible bird’s nest (aerodramus fuciphagus) in processed food by a loop-mediated isothermal amplification (lamp) assay
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298639/
https://www.ncbi.nlm.nih.gov/pubmed/30648568
http://dx.doi.org/10.1016/j.jfda.2018.08.003
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