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Combining Electrochemiluminescence Detection with Aptamer‐Gated Indicator Releasing Mesoporous Nanoparticles Enables ppt Sensitivity for Strip‐Based Rapid Tests

The combination of electrogenerated chemiluminescence (ECL) and aptamer‐gated indicator delivering (gAID) magnetic mesoporous silica nanoparticles embedded into glass fibre paper functionalised with poly(ethyleneglycol) and N‐(3‐triethoxysilylpropyl)diethanolamine allowed the development of a rapid...

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Autores principales: Climent, Estela, Rurack, Knut
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298832/
https://www.ncbi.nlm.nih.gov/pubmed/34595818
http://dx.doi.org/10.1002/anie.202110744
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author Climent, Estela
Rurack, Knut
author_facet Climent, Estela
Rurack, Knut
author_sort Climent, Estela
collection PubMed
description The combination of electrogenerated chemiluminescence (ECL) and aptamer‐gated indicator delivering (gAID) magnetic mesoporous silica nanoparticles embedded into glass fibre paper functionalised with poly(ethyleneglycol) and N‐(3‐triethoxysilylpropyl)diethanolamine allowed the development of a rapid test that detects penicillin directly in diluted milk down to 50±9 ppt in <5 min. Covalent attachment of the aptamer “cap” to the silica scaffold enabled pore closure through non‐covalent electrostatic interactions with surface amino groups, while binding of penicillin led to a folding‐up of the aptamer thus releasing the ECL reporter Ru(bpy)(3) (2+) previously loaded into the material and letting it be detected after lateral flow by a smartphone camera upon electrochemical excitation with a screen printed electrode inserted into a 3D‐printed holder. The approach is simple, generic and presents advantages with respect to sensitivity, measurement uncertainty and robustness compared with conventional fluorescence or electrochemical detection, especially for point‐of‐need analyses of challenging matrices and analytes at ultra‐trace levels.
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spelling pubmed-92988322022-07-21 Combining Electrochemiluminescence Detection with Aptamer‐Gated Indicator Releasing Mesoporous Nanoparticles Enables ppt Sensitivity for Strip‐Based Rapid Tests Climent, Estela Rurack, Knut Angew Chem Int Ed Engl Research Articles The combination of electrogenerated chemiluminescence (ECL) and aptamer‐gated indicator delivering (gAID) magnetic mesoporous silica nanoparticles embedded into glass fibre paper functionalised with poly(ethyleneglycol) and N‐(3‐triethoxysilylpropyl)diethanolamine allowed the development of a rapid test that detects penicillin directly in diluted milk down to 50±9 ppt in <5 min. Covalent attachment of the aptamer “cap” to the silica scaffold enabled pore closure through non‐covalent electrostatic interactions with surface amino groups, while binding of penicillin led to a folding‐up of the aptamer thus releasing the ECL reporter Ru(bpy)(3) (2+) previously loaded into the material and letting it be detected after lateral flow by a smartphone camera upon electrochemical excitation with a screen printed electrode inserted into a 3D‐printed holder. The approach is simple, generic and presents advantages with respect to sensitivity, measurement uncertainty and robustness compared with conventional fluorescence or electrochemical detection, especially for point‐of‐need analyses of challenging matrices and analytes at ultra‐trace levels. John Wiley and Sons Inc. 2021-11-09 2021-12-06 /pmc/articles/PMC9298832/ /pubmed/34595818 http://dx.doi.org/10.1002/anie.202110744 Text en © 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Climent, Estela
Rurack, Knut
Combining Electrochemiluminescence Detection with Aptamer‐Gated Indicator Releasing Mesoporous Nanoparticles Enables ppt Sensitivity for Strip‐Based Rapid Tests
title Combining Electrochemiluminescence Detection with Aptamer‐Gated Indicator Releasing Mesoporous Nanoparticles Enables ppt Sensitivity for Strip‐Based Rapid Tests
title_full Combining Electrochemiluminescence Detection with Aptamer‐Gated Indicator Releasing Mesoporous Nanoparticles Enables ppt Sensitivity for Strip‐Based Rapid Tests
title_fullStr Combining Electrochemiluminescence Detection with Aptamer‐Gated Indicator Releasing Mesoporous Nanoparticles Enables ppt Sensitivity for Strip‐Based Rapid Tests
title_full_unstemmed Combining Electrochemiluminescence Detection with Aptamer‐Gated Indicator Releasing Mesoporous Nanoparticles Enables ppt Sensitivity for Strip‐Based Rapid Tests
title_short Combining Electrochemiluminescence Detection with Aptamer‐Gated Indicator Releasing Mesoporous Nanoparticles Enables ppt Sensitivity for Strip‐Based Rapid Tests
title_sort combining electrochemiluminescence detection with aptamer‐gated indicator releasing mesoporous nanoparticles enables ppt sensitivity for strip‐based rapid tests
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298832/
https://www.ncbi.nlm.nih.gov/pubmed/34595818
http://dx.doi.org/10.1002/anie.202110744
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