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Single-Cell RNA Sequencing Unravels Distinct Tumor Microenvironment of Different Components of Lung Adenocarcinoma Featured as Mixed Ground-Glass Opacity

Lung adenocarcinoma featured as mixed ground-glass opacity (mGGO) doubled its volume half of the time in comparison with that featured as pure ground-glass opacity (pGGO). The mechanisms underlying the heterogeneous appearance of mGGO remain elusive. In this study, we macro-dissected the solid (S) c...

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Autores principales: He, Yu, Yu, Fenglei, Tian, Yi, Hu, Qikang, Wang, Bin, Wang, Li, Hu, Yan, Tao, Yongguang, Chen, Xiaofeng, Peng, Muyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299373/
https://www.ncbi.nlm.nih.gov/pubmed/35874770
http://dx.doi.org/10.3389/fimmu.2022.903513
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author He, Yu
Yu, Fenglei
Tian, Yi
Hu, Qikang
Wang, Bin
Wang, Li
Hu, Yan
Tao, Yongguang
Chen, Xiaofeng
Peng, Muyun
author_facet He, Yu
Yu, Fenglei
Tian, Yi
Hu, Qikang
Wang, Bin
Wang, Li
Hu, Yan
Tao, Yongguang
Chen, Xiaofeng
Peng, Muyun
author_sort He, Yu
collection PubMed
description Lung adenocarcinoma featured as mixed ground-glass opacity (mGGO) doubled its volume half of the time in comparison with that featured as pure ground-glass opacity (pGGO). The mechanisms underlying the heterogeneous appearance of mGGO remain elusive. In this study, we macro-dissected the solid (S) components and ground-glass (GG) components of mGGO and performed single-cell sequencing analyses of six paired components from three mGGO patients. A total of 19,391 single-cell profiles were taken into analysis, and the data of each patient were analyzed independently to obtain a common alteration. Cancer cells and macrophages were the dominant cell types in the S and GG components, respectively. Cancer cells in the S components, which showed relatively malignant phenotypes, were likely to originate from both the GG and S components and monitor the surrounding tumor microenvironment (TME) through an intricate cell interaction network. SPP1 (hi) macrophages were enriched in the S components and showed increased activity of chemoattraction, while macrophages in the GG components displayed an active antimicrobial process with a higher stress-induced state. In addition, the CD47–SIRPA axis was demonstrated to be critical in the maintenance of the GG components. Taken together, our study unraveled the alterations of cell components and transcriptomic features between different components in mGGOs.
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spelling pubmed-92993732022-07-21 Single-Cell RNA Sequencing Unravels Distinct Tumor Microenvironment of Different Components of Lung Adenocarcinoma Featured as Mixed Ground-Glass Opacity He, Yu Yu, Fenglei Tian, Yi Hu, Qikang Wang, Bin Wang, Li Hu, Yan Tao, Yongguang Chen, Xiaofeng Peng, Muyun Front Immunol Immunology Lung adenocarcinoma featured as mixed ground-glass opacity (mGGO) doubled its volume half of the time in comparison with that featured as pure ground-glass opacity (pGGO). The mechanisms underlying the heterogeneous appearance of mGGO remain elusive. In this study, we macro-dissected the solid (S) components and ground-glass (GG) components of mGGO and performed single-cell sequencing analyses of six paired components from three mGGO patients. A total of 19,391 single-cell profiles were taken into analysis, and the data of each patient were analyzed independently to obtain a common alteration. Cancer cells and macrophages were the dominant cell types in the S and GG components, respectively. Cancer cells in the S components, which showed relatively malignant phenotypes, were likely to originate from both the GG and S components and monitor the surrounding tumor microenvironment (TME) through an intricate cell interaction network. SPP1 (hi) macrophages were enriched in the S components and showed increased activity of chemoattraction, while macrophages in the GG components displayed an active antimicrobial process with a higher stress-induced state. In addition, the CD47–SIRPA axis was demonstrated to be critical in the maintenance of the GG components. Taken together, our study unraveled the alterations of cell components and transcriptomic features between different components in mGGOs. Frontiers Media S.A. 2022-07-06 /pmc/articles/PMC9299373/ /pubmed/35874770 http://dx.doi.org/10.3389/fimmu.2022.903513 Text en Copyright © 2022 He, Yu, Tian, Hu, Wang, Wang, Hu, Tao, Chen and Peng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
He, Yu
Yu, Fenglei
Tian, Yi
Hu, Qikang
Wang, Bin
Wang, Li
Hu, Yan
Tao, Yongguang
Chen, Xiaofeng
Peng, Muyun
Single-Cell RNA Sequencing Unravels Distinct Tumor Microenvironment of Different Components of Lung Adenocarcinoma Featured as Mixed Ground-Glass Opacity
title Single-Cell RNA Sequencing Unravels Distinct Tumor Microenvironment of Different Components of Lung Adenocarcinoma Featured as Mixed Ground-Glass Opacity
title_full Single-Cell RNA Sequencing Unravels Distinct Tumor Microenvironment of Different Components of Lung Adenocarcinoma Featured as Mixed Ground-Glass Opacity
title_fullStr Single-Cell RNA Sequencing Unravels Distinct Tumor Microenvironment of Different Components of Lung Adenocarcinoma Featured as Mixed Ground-Glass Opacity
title_full_unstemmed Single-Cell RNA Sequencing Unravels Distinct Tumor Microenvironment of Different Components of Lung Adenocarcinoma Featured as Mixed Ground-Glass Opacity
title_short Single-Cell RNA Sequencing Unravels Distinct Tumor Microenvironment of Different Components of Lung Adenocarcinoma Featured as Mixed Ground-Glass Opacity
title_sort single-cell rna sequencing unravels distinct tumor microenvironment of different components of lung adenocarcinoma featured as mixed ground-glass opacity
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299373/
https://www.ncbi.nlm.nih.gov/pubmed/35874770
http://dx.doi.org/10.3389/fimmu.2022.903513
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