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Beneficial Bacteria in the Intestines of Housefly Larvae Promote Larval Development and Humoral Phenoloxidase Activity, While Harmful Bacteria do the Opposite

The gut microenvironment of houseflies provides unique conditions for microbial colonization. Some gut microorganisms provide benefits for the development of the host by regulating the interaction between the host and intestinal pathogens. Gut microbial alterations can stimulate the host’s immune me...

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Autores principales: Zhang, Qian, Wang, Shumin, Zhang, Xinyu, Zhang, Kexin, Li, Ying, Yin, Yansong, Zhang, Ruiling, Zhang, Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299419/
https://www.ncbi.nlm.nih.gov/pubmed/35874711
http://dx.doi.org/10.3389/fimmu.2022.938972
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author Zhang, Qian
Wang, Shumin
Zhang, Xinyu
Zhang, Kexin
Li, Ying
Yin, Yansong
Zhang, Ruiling
Zhang, Zhong
author_facet Zhang, Qian
Wang, Shumin
Zhang, Xinyu
Zhang, Kexin
Li, Ying
Yin, Yansong
Zhang, Ruiling
Zhang, Zhong
author_sort Zhang, Qian
collection PubMed
description The gut microenvironment of houseflies provides unique conditions for microbial colonization. Some gut microorganisms provide benefits for the development of the host by regulating the interaction between the host and intestinal pathogens. Gut microbial alterations can stimulate the host’s immune mechanism to resist pathogen invasion and affect the development of insects. In this study, we isolated 10 bacterial strains from housefly larval intestines. The isolated bacteria were added to the larval diet to analyze the effects of microecological regulation of gut bacteria on larval development. Dynamic changes in gut flora composition after oral administration of specific bacteria were analyzed although 16S rRNA gene high-throughput sequencing technology. To explore the interaction between gut bacteria and the host, the immune response of larvae against the invasion of foreign microorganisms was observed through a phenoloxidase activity experiment. Our results showed that the oral administration of various isolated bacteria had different effects on larval development. Oral administration of beneficial bacteria, including Enterobacter hormaechei, Klebsiella pneumoniae, Acinetobacter bereziniae, Enterobacter cloacae, Lysinibacillus fusiformis and Bacillus safensis, promoted larval development by increasing gut community diversity and the humoral immunity of larvae, while harmful bacteria, including Pseudomonas aeruginosa, Providencia stuartii and Providencia vermicola, influenced larval development by inhibiting the growth of beneficial bacteria and reducing the humoral immunity of larvae. The beneficial bacteria isolated in our research could be applied as good probiotic additives for the intensive feeding of larvae, while isolation of the harmful bacteria provides a basis for the development of pest inhibitors. Furthermore, our research revealed the immune response of housefly phenoloxidase to exogenous microorganism stimulation, providing richer and more comprehensive knowledge of the larval innate immune response.
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spelling pubmed-92994192022-07-21 Beneficial Bacteria in the Intestines of Housefly Larvae Promote Larval Development and Humoral Phenoloxidase Activity, While Harmful Bacteria do the Opposite Zhang, Qian Wang, Shumin Zhang, Xinyu Zhang, Kexin Li, Ying Yin, Yansong Zhang, Ruiling Zhang, Zhong Front Immunol Immunology The gut microenvironment of houseflies provides unique conditions for microbial colonization. Some gut microorganisms provide benefits for the development of the host by regulating the interaction between the host and intestinal pathogens. Gut microbial alterations can stimulate the host’s immune mechanism to resist pathogen invasion and affect the development of insects. In this study, we isolated 10 bacterial strains from housefly larval intestines. The isolated bacteria were added to the larval diet to analyze the effects of microecological regulation of gut bacteria on larval development. Dynamic changes in gut flora composition after oral administration of specific bacteria were analyzed although 16S rRNA gene high-throughput sequencing technology. To explore the interaction between gut bacteria and the host, the immune response of larvae against the invasion of foreign microorganisms was observed through a phenoloxidase activity experiment. Our results showed that the oral administration of various isolated bacteria had different effects on larval development. Oral administration of beneficial bacteria, including Enterobacter hormaechei, Klebsiella pneumoniae, Acinetobacter bereziniae, Enterobacter cloacae, Lysinibacillus fusiformis and Bacillus safensis, promoted larval development by increasing gut community diversity and the humoral immunity of larvae, while harmful bacteria, including Pseudomonas aeruginosa, Providencia stuartii and Providencia vermicola, influenced larval development by inhibiting the growth of beneficial bacteria and reducing the humoral immunity of larvae. The beneficial bacteria isolated in our research could be applied as good probiotic additives for the intensive feeding of larvae, while isolation of the harmful bacteria provides a basis for the development of pest inhibitors. Furthermore, our research revealed the immune response of housefly phenoloxidase to exogenous microorganism stimulation, providing richer and more comprehensive knowledge of the larval innate immune response. Frontiers Media S.A. 2022-07-06 /pmc/articles/PMC9299419/ /pubmed/35874711 http://dx.doi.org/10.3389/fimmu.2022.938972 Text en Copyright © 2022 Zhang, Wang, Zhang, Zhang, Li, Yin, Zhang and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Zhang, Qian
Wang, Shumin
Zhang, Xinyu
Zhang, Kexin
Li, Ying
Yin, Yansong
Zhang, Ruiling
Zhang, Zhong
Beneficial Bacteria in the Intestines of Housefly Larvae Promote Larval Development and Humoral Phenoloxidase Activity, While Harmful Bacteria do the Opposite
title Beneficial Bacteria in the Intestines of Housefly Larvae Promote Larval Development and Humoral Phenoloxidase Activity, While Harmful Bacteria do the Opposite
title_full Beneficial Bacteria in the Intestines of Housefly Larvae Promote Larval Development and Humoral Phenoloxidase Activity, While Harmful Bacteria do the Opposite
title_fullStr Beneficial Bacteria in the Intestines of Housefly Larvae Promote Larval Development and Humoral Phenoloxidase Activity, While Harmful Bacteria do the Opposite
title_full_unstemmed Beneficial Bacteria in the Intestines of Housefly Larvae Promote Larval Development and Humoral Phenoloxidase Activity, While Harmful Bacteria do the Opposite
title_short Beneficial Bacteria in the Intestines of Housefly Larvae Promote Larval Development and Humoral Phenoloxidase Activity, While Harmful Bacteria do the Opposite
title_sort beneficial bacteria in the intestines of housefly larvae promote larval development and humoral phenoloxidase activity, while harmful bacteria do the opposite
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299419/
https://www.ncbi.nlm.nih.gov/pubmed/35874711
http://dx.doi.org/10.3389/fimmu.2022.938972
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