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Amsterdam urban canals contain novel niches for methane‐cycling microorganisms
Urbanised environments have been identified as hotspots of anthropogenic methane emissions. Especially urban aquatic ecosystems are increasingly recognised as important sources of methane. However, the microbiology behind these emissions remains unexplored. Here, we applied microcosm incubations and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299808/ https://www.ncbi.nlm.nih.gov/pubmed/34863018 http://dx.doi.org/10.1111/1462-2920.15864 |
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author | Pelsma, Koen A. J. in 't Zandt, Michiel H. Op den Camp, Huub J. M. Jetten, Mike S. M. Dean, Joshua F. Welte, Cornelia U. |
author_facet | Pelsma, Koen A. J. in 't Zandt, Michiel H. Op den Camp, Huub J. M. Jetten, Mike S. M. Dean, Joshua F. Welte, Cornelia U. |
author_sort | Pelsma, Koen A. J. |
collection | PubMed |
description | Urbanised environments have been identified as hotspots of anthropogenic methane emissions. Especially urban aquatic ecosystems are increasingly recognised as important sources of methane. However, the microbiology behind these emissions remains unexplored. Here, we applied microcosm incubations and molecular analyses to investigate the methane‐cycling community of the Amsterdam canal system in the Netherlands. The sediment methanogenic communities were dominated by Methanoregulaceae and Methanosaetaceae, with co‐occurring methanotrophic Methanoperedenaceae and Methylomirabilaceae indicating the potential for anaerobic methane oxidation. Methane was readily produced after substrate amendment, suggesting an active but substrate‐limited methanogenic community. Bacterial 16S rRNA gene amplicon sequencing of the sediment revealed a high relative abundance of Thermodesulfovibrionia. Canal wall biofilms showed the highest initial methanotrophic potential under oxic conditions compared to the sediment. During prolonged incubations the maximum methanotrophic rate increased to 8.08 mmol g(DW) (−1) d(−1) that was concomitant with an enrichment of Methylomonadaceae bacteria. Metagenomic analysis of the canal wall biofilm lead to the recovery of a single methanotroph metagenome‐assembled genome. Taxonomic analysis showed that this methanotroph belongs to the genus Methyloglobulus. Our results underline the importance of previously unidentified and specialised environmental niches at the nexus of the natural and human‐impacted carbon cycle. |
format | Online Article Text |
id | pubmed-9299808 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92998082022-07-21 Amsterdam urban canals contain novel niches for methane‐cycling microorganisms Pelsma, Koen A. J. in 't Zandt, Michiel H. Op den Camp, Huub J. M. Jetten, Mike S. M. Dean, Joshua F. Welte, Cornelia U. Environ Microbiol Research Articles Urbanised environments have been identified as hotspots of anthropogenic methane emissions. Especially urban aquatic ecosystems are increasingly recognised as important sources of methane. However, the microbiology behind these emissions remains unexplored. Here, we applied microcosm incubations and molecular analyses to investigate the methane‐cycling community of the Amsterdam canal system in the Netherlands. The sediment methanogenic communities were dominated by Methanoregulaceae and Methanosaetaceae, with co‐occurring methanotrophic Methanoperedenaceae and Methylomirabilaceae indicating the potential for anaerobic methane oxidation. Methane was readily produced after substrate amendment, suggesting an active but substrate‐limited methanogenic community. Bacterial 16S rRNA gene amplicon sequencing of the sediment revealed a high relative abundance of Thermodesulfovibrionia. Canal wall biofilms showed the highest initial methanotrophic potential under oxic conditions compared to the sediment. During prolonged incubations the maximum methanotrophic rate increased to 8.08 mmol g(DW) (−1) d(−1) that was concomitant with an enrichment of Methylomonadaceae bacteria. Metagenomic analysis of the canal wall biofilm lead to the recovery of a single methanotroph metagenome‐assembled genome. Taxonomic analysis showed that this methanotroph belongs to the genus Methyloglobulus. Our results underline the importance of previously unidentified and specialised environmental niches at the nexus of the natural and human‐impacted carbon cycle. John Wiley & Sons, Inc. 2021-12-13 2022-01 /pmc/articles/PMC9299808/ /pubmed/34863018 http://dx.doi.org/10.1111/1462-2920.15864 Text en © 2021 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Pelsma, Koen A. J. in 't Zandt, Michiel H. Op den Camp, Huub J. M. Jetten, Mike S. M. Dean, Joshua F. Welte, Cornelia U. Amsterdam urban canals contain novel niches for methane‐cycling microorganisms |
title | Amsterdam urban canals contain novel niches for methane‐cycling microorganisms |
title_full | Amsterdam urban canals contain novel niches for methane‐cycling microorganisms |
title_fullStr | Amsterdam urban canals contain novel niches for methane‐cycling microorganisms |
title_full_unstemmed | Amsterdam urban canals contain novel niches for methane‐cycling microorganisms |
title_short | Amsterdam urban canals contain novel niches for methane‐cycling microorganisms |
title_sort | amsterdam urban canals contain novel niches for methane‐cycling microorganisms |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299808/ https://www.ncbi.nlm.nih.gov/pubmed/34863018 http://dx.doi.org/10.1111/1462-2920.15864 |
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