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Morphological and distributional properties of SMI‐32 immunoreactive ganglion cells in the rat retina
SMI‐32 is widely used to identify entire populations of alpha retinal ganglion cells (RGCs), and several SMI‐32(+) RGC subsets have been studied thoroughly in rodents. However, due to the thick cover of SMI‐32(+) neurofilaments, the morphology of SMI‐32(+) RGCs in the central retinal region is obscu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299900/ https://www.ncbi.nlm.nih.gov/pubmed/34802150 http://dx.doi.org/10.1002/cne.25275 |
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author | Tan, Huiying Li, Xiaotao Huang, Kang Luo, Moxuan Wang, Liping |
author_facet | Tan, Huiying Li, Xiaotao Huang, Kang Luo, Moxuan Wang, Liping |
author_sort | Tan, Huiying |
collection | PubMed |
description | SMI‐32 is widely used to identify entire populations of alpha retinal ganglion cells (RGCs), and several SMI‐32(+) RGC subsets have been studied thoroughly in rodents. However, due to the thick cover of SMI‐32(+) neurofilaments, the morphology of SMI‐32(+) RGCs in the central retinal region is obscured and rarely described. Moreover, SMI‐32 labels more than one morphological RGC type and the full morphological characteristics and distribution of SMI‐32(+) RGCs have yet to be discovered. Here, using intracellular neurobiotin injections combined with SMI‐32 antibody staining, we investigated morphological and distributional properties of the entire SMI‐32(+) RGCs population in the rat retina. We found that SMI‐32(+) RGCs were evenly distributed throughout the rat retina. We compared the morphological features of SMI‐32(+) ON and OFF cells in the central, middle, and peripheral retinal regions. We found that SMI‐32(+) RGCs in different regions have distinct characteristics, such as the soma area and the dendritic field area, and Sholl analysis of ON cells and OFF cells revealed significant differences between each region. We classified SMI‐32(+) RGCs into five clusters based on morphological features and found that a majority of SMI‐32(+) RGCs belong to alpha‐like cells; however, a small proportion of SMI‐32(+) RGCs had small soma and small dendritic fields. Together, we present a full description of the morphology and distribution of SMI‐32 immunoreactive RGCs in the rat retina. |
format | Online Article Text |
id | pubmed-9299900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92999002022-07-21 Morphological and distributional properties of SMI‐32 immunoreactive ganglion cells in the rat retina Tan, Huiying Li, Xiaotao Huang, Kang Luo, Moxuan Wang, Liping J Comp Neurol Research Articles SMI‐32 is widely used to identify entire populations of alpha retinal ganglion cells (RGCs), and several SMI‐32(+) RGC subsets have been studied thoroughly in rodents. However, due to the thick cover of SMI‐32(+) neurofilaments, the morphology of SMI‐32(+) RGCs in the central retinal region is obscured and rarely described. Moreover, SMI‐32 labels more than one morphological RGC type and the full morphological characteristics and distribution of SMI‐32(+) RGCs have yet to be discovered. Here, using intracellular neurobiotin injections combined with SMI‐32 antibody staining, we investigated morphological and distributional properties of the entire SMI‐32(+) RGCs population in the rat retina. We found that SMI‐32(+) RGCs were evenly distributed throughout the rat retina. We compared the morphological features of SMI‐32(+) ON and OFF cells in the central, middle, and peripheral retinal regions. We found that SMI‐32(+) RGCs in different regions have distinct characteristics, such as the soma area and the dendritic field area, and Sholl analysis of ON cells and OFF cells revealed significant differences between each region. We classified SMI‐32(+) RGCs into five clusters based on morphological features and found that a majority of SMI‐32(+) RGCs belong to alpha‐like cells; however, a small proportion of SMI‐32(+) RGCs had small soma and small dendritic fields. Together, we present a full description of the morphology and distribution of SMI‐32 immunoreactive RGCs in the rat retina. John Wiley and Sons Inc. 2021-12-15 2022-06 /pmc/articles/PMC9299900/ /pubmed/34802150 http://dx.doi.org/10.1002/cne.25275 Text en © 2021 The Authors. The Journal of Comparative Neurology published by Wiley Periodicals LLC https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Tan, Huiying Li, Xiaotao Huang, Kang Luo, Moxuan Wang, Liping Morphological and distributional properties of SMI‐32 immunoreactive ganglion cells in the rat retina |
title | Morphological and distributional properties of SMI‐32 immunoreactive ganglion cells in the rat retina |
title_full | Morphological and distributional properties of SMI‐32 immunoreactive ganglion cells in the rat retina |
title_fullStr | Morphological and distributional properties of SMI‐32 immunoreactive ganglion cells in the rat retina |
title_full_unstemmed | Morphological and distributional properties of SMI‐32 immunoreactive ganglion cells in the rat retina |
title_short | Morphological and distributional properties of SMI‐32 immunoreactive ganglion cells in the rat retina |
title_sort | morphological and distributional properties of smi‐32 immunoreactive ganglion cells in the rat retina |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299900/ https://www.ncbi.nlm.nih.gov/pubmed/34802150 http://dx.doi.org/10.1002/cne.25275 |
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