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Electrochemiluminescence resonance energy transfer biosensing platform between g-C(3)N(4) nanosheet and Ru–SiO(2)@FA for dual-wavelength ratiometric detection of SARS-CoV-2 RdRp gene
Rational detection of syndrome coronavirus 2 (SARS-CoV-2) is crucial to prevention, control, and treatment of disease. Herein, a dual-wavelength ratiometric electrochemiluminescence (ECL) biosensor based on resonance energy transfer (RET) between g-C(3)N(4) nanosheets and Ru–SiO(2)@folic acid (FA) n...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299981/ https://www.ncbi.nlm.nih.gov/pubmed/35917609 http://dx.doi.org/10.1016/j.bios.2022.114580 |
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author | Yin, Tengyue Ye, Yuhang Dong, Wenshuai Jie, Guifen |
author_facet | Yin, Tengyue Ye, Yuhang Dong, Wenshuai Jie, Guifen |
author_sort | Yin, Tengyue |
collection | PubMed |
description | Rational detection of syndrome coronavirus 2 (SARS-CoV-2) is crucial to prevention, control, and treatment of disease. Herein, a dual-wavelength ratiometric electrochemiluminescence (ECL) biosensor based on resonance energy transfer (RET) between g-C(3)N(4) nanosheets and Ru–SiO(2)@folic acid (FA) nanomaterials was designed to realize ultrasensitive detection of SARS-CoV-2 virus (RdRp gene). Firstly, the unique g-C(3)N(4) nanosheets displayed very intense and stable ECL at 460 nm, then the triple helix DNA was stably and vertically bound to g-C(3)N(4) on electrode by high binding affinity between ssDNA and g-C(3)N(4). Meanwhile, trace amounts of target genes were converted to a large number of output by three-dimensional (3D) DNA walker multiple amplification, and the output bridged a multifunctional probe Ru–SiO(2)@FA to electrode. Ru–SiO(2)@FA not only showed high ECL at 620 nm, but also effectively quenched g-C(3)N(4) ECL. As a result, ECL decreased at 460 nm and increased at 620 nm, which was used to design a rational ECL biosensor for detection of SARS gene. The results show that the biosensor has excellent detection sensitivity for RdRp gene with a dynamic detection range of 1 fM to 10 nM and a limit of detection (LOD) of 0.18 fM. The dual-wavelength ratio ECL biosensor has inestimable value and application prospects in the fields of biosensing and clinical diagnosis. |
format | Online Article Text |
id | pubmed-9299981 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92999812022-07-21 Electrochemiluminescence resonance energy transfer biosensing platform between g-C(3)N(4) nanosheet and Ru–SiO(2)@FA for dual-wavelength ratiometric detection of SARS-CoV-2 RdRp gene Yin, Tengyue Ye, Yuhang Dong, Wenshuai Jie, Guifen Biosens Bioelectron Article Rational detection of syndrome coronavirus 2 (SARS-CoV-2) is crucial to prevention, control, and treatment of disease. Herein, a dual-wavelength ratiometric electrochemiluminescence (ECL) biosensor based on resonance energy transfer (RET) between g-C(3)N(4) nanosheets and Ru–SiO(2)@folic acid (FA) nanomaterials was designed to realize ultrasensitive detection of SARS-CoV-2 virus (RdRp gene). Firstly, the unique g-C(3)N(4) nanosheets displayed very intense and stable ECL at 460 nm, then the triple helix DNA was stably and vertically bound to g-C(3)N(4) on electrode by high binding affinity between ssDNA and g-C(3)N(4). Meanwhile, trace amounts of target genes were converted to a large number of output by three-dimensional (3D) DNA walker multiple amplification, and the output bridged a multifunctional probe Ru–SiO(2)@FA to electrode. Ru–SiO(2)@FA not only showed high ECL at 620 nm, but also effectively quenched g-C(3)N(4) ECL. As a result, ECL decreased at 460 nm and increased at 620 nm, which was used to design a rational ECL biosensor for detection of SARS gene. The results show that the biosensor has excellent detection sensitivity for RdRp gene with a dynamic detection range of 1 fM to 10 nM and a limit of detection (LOD) of 0.18 fM. The dual-wavelength ratio ECL biosensor has inestimable value and application prospects in the fields of biosensing and clinical diagnosis. Elsevier B.V. 2022-11-01 2022-07-21 /pmc/articles/PMC9299981/ /pubmed/35917609 http://dx.doi.org/10.1016/j.bios.2022.114580 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Yin, Tengyue Ye, Yuhang Dong, Wenshuai Jie, Guifen Electrochemiluminescence resonance energy transfer biosensing platform between g-C(3)N(4) nanosheet and Ru–SiO(2)@FA for dual-wavelength ratiometric detection of SARS-CoV-2 RdRp gene |
title | Electrochemiluminescence resonance energy transfer biosensing platform between g-C(3)N(4) nanosheet and Ru–SiO(2)@FA for dual-wavelength ratiometric detection of SARS-CoV-2 RdRp gene |
title_full | Electrochemiluminescence resonance energy transfer biosensing platform between g-C(3)N(4) nanosheet and Ru–SiO(2)@FA for dual-wavelength ratiometric detection of SARS-CoV-2 RdRp gene |
title_fullStr | Electrochemiluminescence resonance energy transfer biosensing platform between g-C(3)N(4) nanosheet and Ru–SiO(2)@FA for dual-wavelength ratiometric detection of SARS-CoV-2 RdRp gene |
title_full_unstemmed | Electrochemiluminescence resonance energy transfer biosensing platform between g-C(3)N(4) nanosheet and Ru–SiO(2)@FA for dual-wavelength ratiometric detection of SARS-CoV-2 RdRp gene |
title_short | Electrochemiluminescence resonance energy transfer biosensing platform between g-C(3)N(4) nanosheet and Ru–SiO(2)@FA for dual-wavelength ratiometric detection of SARS-CoV-2 RdRp gene |
title_sort | electrochemiluminescence resonance energy transfer biosensing platform between g-c(3)n(4) nanosheet and ru–sio(2)@fa for dual-wavelength ratiometric detection of sars-cov-2 rdrp gene |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299981/ https://www.ncbi.nlm.nih.gov/pubmed/35917609 http://dx.doi.org/10.1016/j.bios.2022.114580 |
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