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Clinical comparison and agreement of PCR, antigen, and viral culture for the diagnosis of COVID-19: Clinical Agreement Between Diagnostics for COVID19

The aim of this study is to compare the COVID-19 nasopharyngeal PCR (NP PCR) to antigen, nasal PCR, and viral culture. One-hundred-and-fourteen risk-stratified patients were tested by culture, nasal PCR, NP PCR, and Ag testing. Twenty (48%) of the high risk and 23 (32%) of the low risk were NP PCR p...

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Autores principales: Agard, Amanda, Elsheikh, Omar, Bell, Drew, Relich, Ryan F., Schmitt, Bryan H., Sadowski, Josh, Fadel, William, Webb, Douglas H., Dbeibo, Lana, Kelley, Kristen, Carozza, Mariel, Lei, Guang-Shen, Calkins, Paul, Beeler, Cole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier Ltd. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300048/
https://www.ncbi.nlm.nih.gov/pubmed/35880110
http://dx.doi.org/10.1016/j.jcvp.2022.100099
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author Agard, Amanda
Elsheikh, Omar
Bell, Drew
Relich, Ryan F.
Schmitt, Bryan H.
Sadowski, Josh
Fadel, William
Webb, Douglas H.
Dbeibo, Lana
Kelley, Kristen
Carozza, Mariel
Lei, Guang-Shen
Calkins, Paul
Beeler, Cole
author_facet Agard, Amanda
Elsheikh, Omar
Bell, Drew
Relich, Ryan F.
Schmitt, Bryan H.
Sadowski, Josh
Fadel, William
Webb, Douglas H.
Dbeibo, Lana
Kelley, Kristen
Carozza, Mariel
Lei, Guang-Shen
Calkins, Paul
Beeler, Cole
author_sort Agard, Amanda
collection PubMed
description The aim of this study is to compare the COVID-19 nasopharyngeal PCR (NP PCR) to antigen, nasal PCR, and viral culture. One-hundred-and-fourteen risk-stratified patients were tested by culture, nasal PCR, NP PCR, and Ag testing. Twenty (48%) of the high risk and 23 (32%) of the low risk were NP PCR positive. Compared with NP PCR, the sensitivity of nasal PCR, Sofia Ag, BinaxNOW Ag, and culture were 44%, 31%, 37%, and 15%. In the high risk group, the sensitivity of these tests improved to 71%, 37%, 50%, and 22%. Agreement between tests was highest between nasal PCR and both antigen tests. Patients who were NP PCR positive but antigen negative were more likely to have remote prior COVID-19 infection (p<0.01). Nasal PCR and antigen positive patients were more likely to have symptoms (p = 0.01).
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spelling pubmed-93000482022-07-21 Clinical comparison and agreement of PCR, antigen, and viral culture for the diagnosis of COVID-19: Clinical Agreement Between Diagnostics for COVID19 Agard, Amanda Elsheikh, Omar Bell, Drew Relich, Ryan F. Schmitt, Bryan H. Sadowski, Josh Fadel, William Webb, Douglas H. Dbeibo, Lana Kelley, Kristen Carozza, Mariel Lei, Guang-Shen Calkins, Paul Beeler, Cole J Clin Virol Plus Article The aim of this study is to compare the COVID-19 nasopharyngeal PCR (NP PCR) to antigen, nasal PCR, and viral culture. One-hundred-and-fourteen risk-stratified patients were tested by culture, nasal PCR, NP PCR, and Ag testing. Twenty (48%) of the high risk and 23 (32%) of the low risk were NP PCR positive. Compared with NP PCR, the sensitivity of nasal PCR, Sofia Ag, BinaxNOW Ag, and culture were 44%, 31%, 37%, and 15%. In the high risk group, the sensitivity of these tests improved to 71%, 37%, 50%, and 22%. Agreement between tests was highest between nasal PCR and both antigen tests. Patients who were NP PCR positive but antigen negative were more likely to have remote prior COVID-19 infection (p<0.01). Nasal PCR and antigen positive patients were more likely to have symptoms (p = 0.01). The Author(s). Published by Elsevier Ltd. 2022-08 2022-07-21 /pmc/articles/PMC9300048/ /pubmed/35880110 http://dx.doi.org/10.1016/j.jcvp.2022.100099 Text en © 2022 The Author(s). Published by Elsevier Ltd. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Agard, Amanda
Elsheikh, Omar
Bell, Drew
Relich, Ryan F.
Schmitt, Bryan H.
Sadowski, Josh
Fadel, William
Webb, Douglas H.
Dbeibo, Lana
Kelley, Kristen
Carozza, Mariel
Lei, Guang-Shen
Calkins, Paul
Beeler, Cole
Clinical comparison and agreement of PCR, antigen, and viral culture for the diagnosis of COVID-19: Clinical Agreement Between Diagnostics for COVID19
title Clinical comparison and agreement of PCR, antigen, and viral culture for the diagnosis of COVID-19: Clinical Agreement Between Diagnostics for COVID19
title_full Clinical comparison and agreement of PCR, antigen, and viral culture for the diagnosis of COVID-19: Clinical Agreement Between Diagnostics for COVID19
title_fullStr Clinical comparison and agreement of PCR, antigen, and viral culture for the diagnosis of COVID-19: Clinical Agreement Between Diagnostics for COVID19
title_full_unstemmed Clinical comparison and agreement of PCR, antigen, and viral culture for the diagnosis of COVID-19: Clinical Agreement Between Diagnostics for COVID19
title_short Clinical comparison and agreement of PCR, antigen, and viral culture for the diagnosis of COVID-19: Clinical Agreement Between Diagnostics for COVID19
title_sort clinical comparison and agreement of pcr, antigen, and viral culture for the diagnosis of covid-19: clinical agreement between diagnostics for covid19
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300048/
https://www.ncbi.nlm.nih.gov/pubmed/35880110
http://dx.doi.org/10.1016/j.jcvp.2022.100099
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