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cis‐prenyltransferase 3 and α/β‐hydrolase are new determinants of dolichol accumulation in Arabidopsis

Dolichols (Dols), ubiquitous components of living organisms, are indispensable for cell survival. In plants, as well as other eukaryotes, Dols are crucial for post‐translational protein glycosylation, aberration of which leads to fatal metabolic disorders in humans and male sterility in plants. Unti...

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Autores principales: Gawarecka, Katarzyna, Siwinska, Joanna, Poznanski, Jaroslaw, Onysk, Agnieszka, Surowiecki, Przemyslaw, Sztompka, Karolina, Surmacz, Liliana, Ahn, Ji Hoon, Korte, Arthur, Swiezewska, Ewa, Ihnatowicz, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300173/
https://www.ncbi.nlm.nih.gov/pubmed/34778961
http://dx.doi.org/10.1111/pce.14223
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author Gawarecka, Katarzyna
Siwinska, Joanna
Poznanski, Jaroslaw
Onysk, Agnieszka
Surowiecki, Przemyslaw
Sztompka, Karolina
Surmacz, Liliana
Ahn, Ji Hoon
Korte, Arthur
Swiezewska, Ewa
Ihnatowicz, Anna
author_facet Gawarecka, Katarzyna
Siwinska, Joanna
Poznanski, Jaroslaw
Onysk, Agnieszka
Surowiecki, Przemyslaw
Sztompka, Karolina
Surmacz, Liliana
Ahn, Ji Hoon
Korte, Arthur
Swiezewska, Ewa
Ihnatowicz, Anna
author_sort Gawarecka, Katarzyna
collection PubMed
description Dolichols (Dols), ubiquitous components of living organisms, are indispensable for cell survival. In plants, as well as other eukaryotes, Dols are crucial for post‐translational protein glycosylation, aberration of which leads to fatal metabolic disorders in humans and male sterility in plants. Until now, the mechanisms underlying Dol accumulation remain elusive. In this study, we have analysed the natural variation of the accumulation of Dols and six other isoprenoids among more than 120 Arabidopsis thaliana accessions. Subsequently, by combining QTL and GWAS approaches, we have identified several candidate genes involved in the accumulation of Dols, polyprenols, plastoquinone and phytosterols. The role of two genes implicated in the accumulation of major Dols in Arabidopsis—the AT2G17570 gene encoding a long searched for cis‐prenyltransferase (CPT3) and the AT1G52460 gene encoding an α/β‐hydrolase—is experimentally confirmed. These data will help to generate Dol‐enriched plants which might serve as a remedy for Dol‐deficiency in humans.
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spelling pubmed-93001732022-07-21 cis‐prenyltransferase 3 and α/β‐hydrolase are new determinants of dolichol accumulation in Arabidopsis Gawarecka, Katarzyna Siwinska, Joanna Poznanski, Jaroslaw Onysk, Agnieszka Surowiecki, Przemyslaw Sztompka, Karolina Surmacz, Liliana Ahn, Ji Hoon Korte, Arthur Swiezewska, Ewa Ihnatowicz, Anna Plant Cell Environ Original Articles Dolichols (Dols), ubiquitous components of living organisms, are indispensable for cell survival. In plants, as well as other eukaryotes, Dols are crucial for post‐translational protein glycosylation, aberration of which leads to fatal metabolic disorders in humans and male sterility in plants. Until now, the mechanisms underlying Dol accumulation remain elusive. In this study, we have analysed the natural variation of the accumulation of Dols and six other isoprenoids among more than 120 Arabidopsis thaliana accessions. Subsequently, by combining QTL and GWAS approaches, we have identified several candidate genes involved in the accumulation of Dols, polyprenols, plastoquinone and phytosterols. The role of two genes implicated in the accumulation of major Dols in Arabidopsis—the AT2G17570 gene encoding a long searched for cis‐prenyltransferase (CPT3) and the AT1G52460 gene encoding an α/β‐hydrolase—is experimentally confirmed. These data will help to generate Dol‐enriched plants which might serve as a remedy for Dol‐deficiency in humans. John Wiley and Sons Inc. 2021-11-29 2022-02 /pmc/articles/PMC9300173/ /pubmed/34778961 http://dx.doi.org/10.1111/pce.14223 Text en © 2021 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Gawarecka, Katarzyna
Siwinska, Joanna
Poznanski, Jaroslaw
Onysk, Agnieszka
Surowiecki, Przemyslaw
Sztompka, Karolina
Surmacz, Liliana
Ahn, Ji Hoon
Korte, Arthur
Swiezewska, Ewa
Ihnatowicz, Anna
cis‐prenyltransferase 3 and α/β‐hydrolase are new determinants of dolichol accumulation in Arabidopsis
title cis‐prenyltransferase 3 and α/β‐hydrolase are new determinants of dolichol accumulation in Arabidopsis
title_full cis‐prenyltransferase 3 and α/β‐hydrolase are new determinants of dolichol accumulation in Arabidopsis
title_fullStr cis‐prenyltransferase 3 and α/β‐hydrolase are new determinants of dolichol accumulation in Arabidopsis
title_full_unstemmed cis‐prenyltransferase 3 and α/β‐hydrolase are new determinants of dolichol accumulation in Arabidopsis
title_short cis‐prenyltransferase 3 and α/β‐hydrolase are new determinants of dolichol accumulation in Arabidopsis
title_sort cis‐prenyltransferase 3 and α/β‐hydrolase are new determinants of dolichol accumulation in arabidopsis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300173/
https://www.ncbi.nlm.nih.gov/pubmed/34778961
http://dx.doi.org/10.1111/pce.14223
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