Circulating Exosomes from Mice with LPS-Induced Bone Loss Inhibit Osteoblast Differentiation

Osteoimmunology focuses on the intermodulation between bone and the immune system. Lipopolysaccharide (LPS)-induced bone loss models are commonly used to investigate the interface between inflammation and osteoporosis. Circulating exosomes can regulate physiological and pathological processes throug...

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Detalles Bibliográficos
Autores principales: Wang, Yixuan, Zhang, Lijun, Wang, Ke, Zhou, Hua, Li, Gaozhi, Xu, Liqun, Hu, Zebing, Cao, Xinsheng, Shi, Fei, Zhang, Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300544/
https://www.ncbi.nlm.nih.gov/pubmed/35435443
http://dx.doi.org/10.1007/s00223-022-00977-x
Descripción
Sumario:Osteoimmunology focuses on the intermodulation between bone and the immune system. Lipopolysaccharide (LPS)-induced bone loss models are commonly used to investigate the interface between inflammation and osteoporosis. Circulating exosomes can regulate physiological and pathological processes through exosomal microRNAs and proteins. In this study, we observed reduced osteoblast number and bone formation in LPS-induced bone loss mice (LPS mice). Levels of circulating exosomes were increased by ~ twofold in LPS mice, and the expression of exosomal miRNAs was significantly changed. miRNAs (miRNA-125b-5p, miRNA-132-3p, and miRNA-214-3p) that were reported to inhibit osteoblast activity were significantly increased in the serum exosomes and bone tissues of LPS mice. Additionally, LPS-induced increases in exosomes significantly inhibited the osteogenic differentiation of MC3T3-E1 cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00223-022-00977-x.

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