Circulating Exosomes from Mice with LPS-Induced Bone Loss Inhibit Osteoblast Differentiation

Osteoimmunology focuses on the intermodulation between bone and the immune system. Lipopolysaccharide (LPS)-induced bone loss models are commonly used to investigate the interface between inflammation and osteoporosis. Circulating exosomes can regulate physiological and pathological processes throug...

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Autores principales: Wang, Yixuan, Zhang, Lijun, Wang, Ke, Zhou, Hua, Li, Gaozhi, Xu, Liqun, Hu, Zebing, Cao, Xinsheng, Shi, Fei, Zhang, Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300544/
https://www.ncbi.nlm.nih.gov/pubmed/35435443
http://dx.doi.org/10.1007/s00223-022-00977-x
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author Wang, Yixuan
Zhang, Lijun
Wang, Ke
Zhou, Hua
Li, Gaozhi
Xu, Liqun
Hu, Zebing
Cao, Xinsheng
Shi, Fei
Zhang, Shu
author_facet Wang, Yixuan
Zhang, Lijun
Wang, Ke
Zhou, Hua
Li, Gaozhi
Xu, Liqun
Hu, Zebing
Cao, Xinsheng
Shi, Fei
Zhang, Shu
author_sort Wang, Yixuan
collection PubMed
description Osteoimmunology focuses on the intermodulation between bone and the immune system. Lipopolysaccharide (LPS)-induced bone loss models are commonly used to investigate the interface between inflammation and osteoporosis. Circulating exosomes can regulate physiological and pathological processes through exosomal microRNAs and proteins. In this study, we observed reduced osteoblast number and bone formation in LPS-induced bone loss mice (LPS mice). Levels of circulating exosomes were increased by ~ twofold in LPS mice, and the expression of exosomal miRNAs was significantly changed. miRNAs (miRNA-125b-5p, miRNA-132-3p, and miRNA-214-3p) that were reported to inhibit osteoblast activity were significantly increased in the serum exosomes and bone tissues of LPS mice. Additionally, LPS-induced increases in exosomes significantly inhibited the osteogenic differentiation of MC3T3-E1 cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00223-022-00977-x.
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spelling pubmed-93005442022-07-22 Circulating Exosomes from Mice with LPS-Induced Bone Loss Inhibit Osteoblast Differentiation Wang, Yixuan Zhang, Lijun Wang, Ke Zhou, Hua Li, Gaozhi Xu, Liqun Hu, Zebing Cao, Xinsheng Shi, Fei Zhang, Shu Calcif Tissue Int Original Research Osteoimmunology focuses on the intermodulation between bone and the immune system. Lipopolysaccharide (LPS)-induced bone loss models are commonly used to investigate the interface between inflammation and osteoporosis. Circulating exosomes can regulate physiological and pathological processes through exosomal microRNAs and proteins. In this study, we observed reduced osteoblast number and bone formation in LPS-induced bone loss mice (LPS mice). Levels of circulating exosomes were increased by ~ twofold in LPS mice, and the expression of exosomal miRNAs was significantly changed. miRNAs (miRNA-125b-5p, miRNA-132-3p, and miRNA-214-3p) that were reported to inhibit osteoblast activity were significantly increased in the serum exosomes and bone tissues of LPS mice. Additionally, LPS-induced increases in exosomes significantly inhibited the osteogenic differentiation of MC3T3-E1 cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00223-022-00977-x. Springer US 2022-04-18 2022 /pmc/articles/PMC9300544/ /pubmed/35435443 http://dx.doi.org/10.1007/s00223-022-00977-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Research
Wang, Yixuan
Zhang, Lijun
Wang, Ke
Zhou, Hua
Li, Gaozhi
Xu, Liqun
Hu, Zebing
Cao, Xinsheng
Shi, Fei
Zhang, Shu
Circulating Exosomes from Mice with LPS-Induced Bone Loss Inhibit Osteoblast Differentiation
title Circulating Exosomes from Mice with LPS-Induced Bone Loss Inhibit Osteoblast Differentiation
title_full Circulating Exosomes from Mice with LPS-Induced Bone Loss Inhibit Osteoblast Differentiation
title_fullStr Circulating Exosomes from Mice with LPS-Induced Bone Loss Inhibit Osteoblast Differentiation
title_full_unstemmed Circulating Exosomes from Mice with LPS-Induced Bone Loss Inhibit Osteoblast Differentiation
title_short Circulating Exosomes from Mice with LPS-Induced Bone Loss Inhibit Osteoblast Differentiation
title_sort circulating exosomes from mice with lps-induced bone loss inhibit osteoblast differentiation
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300544/
https://www.ncbi.nlm.nih.gov/pubmed/35435443
http://dx.doi.org/10.1007/s00223-022-00977-x
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