Cargando…

Infecting kidney organoids with a cDNA reporter clone of SARS-CoV-2

Induced pluripotent stem cell (iPSC)-derived kidney organoids can be used for disease modeling and drug testing. Here, we describe a protocol to prepare stocks of an infectious clone of SARS-CoV-2 expressing a stable mNeonGreen reporter (icSARS-CoV-2-mNG). We demonstrate the infection of kidney orga...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chung, Hyunjae, Bui-Marinos, Maxwell P., Rahmani, Waleed, Corcoran, Jennifer A., Chun, Justin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300580/ https://www.ncbi.nlm.nih.gov/pubmed/35990736 http://dx.doi.org/10.1016/j.xpro.2022.101617

Ejemplares similares

Attenuation of SARS-CoV-2 infection by losartan in human kidney organoids
por: Rahmani, Waleed, et al.
Publicado: (2022)

An Infectious cDNA Clone of SARS-CoV-2
por: Xie, Xuping, et al.
Publicado: (2020)

Protocol for SARS-CoV-2 infection of kidney organoids derived from human pluripotent stem cells
por: Garreta, Elena, et al.
Publicado: (2022)

Cloning and sequencing of murine T3 gamma cDNA from a subtractive cDNA library
Publicado: (1987)

Functional cDNA expression cloning: Pushing it to the limit
por: OKAYAMA, Hiroto
Publicado: (2012)

Using human iPSC-derived kidney organoids to decipher SARS-CoV-2 pathology on single cell level
por: Reimer, Katharina C., et al.
Publicado: (2022)

The full-ORF clone resource of the German cDNA Consortium
por: Bechtel, Stephanie, et al.
Publicado: (2007)

Rescue of a Cilevirus from infectious cDNA clones
por: Leastro, Mikhail Oliveira, et al.
Publicado: (2023)

Protocol for qPCR analysis that corrects for cDNA amplification efficiency
por: Damgaard, Mads V., et al.
Publicado: (2022)

Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids
por: Eriksen, Anne Zebitz, et al.
Publicado: (2022)

Rapid identification of overlapping cDNA clones by Southern cross-hybridization.
por: Jouvenne, P, et al.
Publicado: (1988)

Molecular cloning of cDNA for rat liver gap junction protein
Publicado: (1986)

Development of infectious cDNA clones of Salmonid alphavirus subtype 3
por: Karlsen, Marius, et al.
Publicado: (2010)

The preparation of an infectious full-length cDNA clone of Saffold virus
por: Himeda, Toshiki, et al.
Publicado: (2011)

Molecular cloning and sequence analysis of hamster CENP-A cDNA
por: Figueroa, Javier, et al.
Publicado: (2002)

Cloning and Sequence Analysis of Wild Argali ISG15 cDNA
por: Sun, Yanming, et al.
Publicado: (2014)

Construction and characterization of an infectious cDNA clone of coxsackievirus A 10
por: Liu, Qiliang, et al.
Publicado: (2019)

Lemon protein disulfide isomerase: cDNA cloning and biochemical characterization
por: Chen, Yu-Ting, et al.
Publicado: (2013)

Construction and verification of an infectious cDNA clone of coxsackievirus B5
por: Song, Lifang, et al.
Publicado: (2022)

Sequencing and Analysis of Approximately 40 000 Soybean cDNA Clones from a Full-Length-Enriched cDNA Library
por: Umezawa, Taishi, et al.
Publicado: (2008)

Full-length infectious cDNA clone of Middle East respiratory syndrome coronavirus (MERS-CoV)
Publicado: (2013)

Nsp1 proteins of human coronaviruses HCoV-OC43 and SARS-CoV2 inhibit stress granule formation
por: Dolliver, Stacia M., et al.
Publicado: (2022)

Integrating De Novo Transcriptome Assembly and Cloning to Obtain Chicken Ovocleidin-17 Full-Length cDNA
por: Zhang, Quan, et al.
Publicado: (2014)

Denervation supersensitivity in skeletal muscle: analysis with a cloned cDNA probe
Publicado: (1984)

Functional expression of the polymeric immunoglobulin receptor from cloned cDNA in fibroblasts
Publicado: (1986)

Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen
Publicado: (1987)

Molecular Cloning and Characterization of an Acetylcholinesterase cDNA in the Brown Planthopper, Nilaparvata lugens
por: Yang, Zhifan, et al.
Publicado: (2010)

Revisiting the Identification and cDNA Cloning of T Cell-Replacing Factor/Interleukin-5
por: Takatsu, Kiyoshi
Publicado: (2014)

Cloning of Mouse DAN cDNA and Its Down‐regulation in Transformed Cells
por: Ozaki, Toshinori, et al.
Publicado: (1996)

Toward the Development of an Infectious cDNA Clone of a Human Enteric Coronavirus
por: Zhu, Hongqing, et al.
Publicado: (2006)

An efficient method to generate kidney organoids at the air-liquid interface
por: Gupta, Ashwani Kumar, et al.
Publicado: (2021)

Injection and electroporation of plasmid DNA into human cortical organoids
por: Denoth-Lippuner, Annina, et al.
Publicado: (2022)

Cloning and sequence analysis of Wild Argali short palate, lung and nasal epithelium clone 1 cDNA
por: Shen, Wen, et al.
Publicado: (2017)

An Efficient Method to Prepare Barcoded cDNA Libraries from Plant Callus for Long-Read Sequencing
por: Cordeiro, Daniela, et al.
Publicado: (2023)

Cloning of DNA oligo pools for in vitro expression
por: Uzonyi, Anna, et al.
Publicado: (2022)

In vivo gain-of-function cDNA library screening for colonization genes in a mouse model of pulmonary metastasis
por: Tu, Zhenbo, et al.
Publicado: (2022)

Isolation of a lactoferrin cDNA clone and its expression in human breast cancer.
por: Campbell, T., et al.
Publicado: (1992)

Cloning of a novel inhibin alpha cDNA from rhesus monkey testis
por: Bernard, Daniel J, et al.
Publicado: (2004)

A catalog of human cDNA expression clones and its application to structural genomics
por: Büssow, Konrad, et al.
Publicado: (2004)

Cloning and endogenous expression of a Eucalyptus grandis UDP-glucose dehydrogenase cDNA
por: Labate, Mônica T. Veneziano, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_for69agsgpdilhlerc0f94at63