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Cells responding to chemoattractant on a structured substrate
Cell migration on an adhesive substrate surface comprises actin-based protrusion at the front and retraction of the tail in combination with coordinated adhesion to, and detachment from, the substrate. To study the effect of cell-to-substrate adhesion on the chemotactic response of Dictyostelium dis...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300663/ https://www.ncbi.nlm.nih.gov/pubmed/35644945 http://dx.doi.org/10.1016/j.bpj.2022.05.043 |
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author | Rußbach, Laura Ecke, Mary Rädler, Joachim O. Leu, Charlott Gerisch, Günther |
author_facet | Rußbach, Laura Ecke, Mary Rädler, Joachim O. Leu, Charlott Gerisch, Günther |
author_sort | Rußbach, Laura |
collection | PubMed |
description | Cell migration on an adhesive substrate surface comprises actin-based protrusion at the front and retraction of the tail in combination with coordinated adhesion to, and detachment from, the substrate. To study the effect of cell-to-substrate adhesion on the chemotactic response of Dictyostelium discoideum cells, we exposed the cells to patterned substrate surfaces consisting of adhesive and inert areas, and forced them by a gradient of chemoattractant to enter the border between the two areas. Wild-type as well as myosin II-deficient cells stop at the border of an adhesive area. They do not detach with their rear part, while on the nonadhesive area they protrude pseudopods at their front toward the source of chemoattractant. Avoidance of the nonadhesive area may cause a cell to move in tangential direction relative to the attractant gradient, keeping its tail at the border of the adhesive surface. |
format | Online Article Text |
id | pubmed-9300663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Biophysical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-93006632023-07-05 Cells responding to chemoattractant on a structured substrate Rußbach, Laura Ecke, Mary Rädler, Joachim O. Leu, Charlott Gerisch, Günther Biophys J Article Cell migration on an adhesive substrate surface comprises actin-based protrusion at the front and retraction of the tail in combination with coordinated adhesion to, and detachment from, the substrate. To study the effect of cell-to-substrate adhesion on the chemotactic response of Dictyostelium discoideum cells, we exposed the cells to patterned substrate surfaces consisting of adhesive and inert areas, and forced them by a gradient of chemoattractant to enter the border between the two areas. Wild-type as well as myosin II-deficient cells stop at the border of an adhesive area. They do not detach with their rear part, while on the nonadhesive area they protrude pseudopods at their front toward the source of chemoattractant. Avoidance of the nonadhesive area may cause a cell to move in tangential direction relative to the attractant gradient, keeping its tail at the border of the adhesive surface. The Biophysical Society 2022-07-05 2022-05-28 /pmc/articles/PMC9300663/ /pubmed/35644945 http://dx.doi.org/10.1016/j.bpj.2022.05.043 Text en © 2022 Biophysical Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Rußbach, Laura Ecke, Mary Rädler, Joachim O. Leu, Charlott Gerisch, Günther Cells responding to chemoattractant on a structured substrate |
title | Cells responding to chemoattractant on a structured substrate |
title_full | Cells responding to chemoattractant on a structured substrate |
title_fullStr | Cells responding to chemoattractant on a structured substrate |
title_full_unstemmed | Cells responding to chemoattractant on a structured substrate |
title_short | Cells responding to chemoattractant on a structured substrate |
title_sort | cells responding to chemoattractant on a structured substrate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300663/ https://www.ncbi.nlm.nih.gov/pubmed/35644945 http://dx.doi.org/10.1016/j.bpj.2022.05.043 |
work_keys_str_mv | AT rußbachlaura cellsrespondingtochemoattractantonastructuredsubstrate AT eckemary cellsrespondingtochemoattractantonastructuredsubstrate AT radlerjoachimo cellsrespondingtochemoattractantonastructuredsubstrate AT leucharlott cellsrespondingtochemoattractantonastructuredsubstrate AT gerischgunther cellsrespondingtochemoattractantonastructuredsubstrate |